Features of Prostate Gland Morphogenesis in 8-10-Month-Old Human Fetuses

2019 ◽  
Vol 4 (5) ◽  
pp. 90-95
Author(s):  
T. V. Khmara ◽  
◽  
I. I. Zamorskii ◽  
V. V. Vizniuk ◽  
М. P. Kavun ◽  
...  
Author(s):  
Bruce Wetzel ◽  
Robert Buscho ◽  
Raphael Dolin

It has been reported that explants of human fetal intestine can be maintained in culture for up to 21 days in a viable condition and that these organ cultures support the growth of a variety of known viral agents responsible for enteric disease. Scanning electron microscopy (SEM) has been undertaken on several series of these explants to determine their appearance under routine culture conditions.Fresh specimens of jejunum obtained from normal human fetuses were washed, dissected into l-4mm pieces, and cultured in modified Leibowitz L-15 medium at 34° C as previously described. Serial specimens were fixed each day in 3% glutaraldehyde for 90 minutes at room temperature, rinsed, dehydrated, and dried by the CO2 critical point method in a Denton DCP-1 device. Specimens were attached to aluminum stubs with 3M transfer tape No. 465, and one sample on each stub was carefully rolled along the adhesive such that villi were broken off to expose their interiors.


Author(s):  
José A. Serrano ◽  
Hannah L. Wasserkrug ◽  
Anna A. Serrano ◽  
Arnold M. Seligman

As previously reported (1, 2) phosphorylcholine (PC) is a specific substrate for prostatatic acid phosphatase (PAP) as opposed to other acid phosphatases, e.g., lysosomal acid phosphatase. The specificity of PC for PAP is due to the pentavalent nitrogen in PC, a feature that renders PC resistant to hydrolysis by all other acid phosphatases. Detailed comparative cytochemical results in rat tissues are in press. This report deals with ultracytochemical results applying the method to normal and pathological human prostate gland.Fresh human prostate was obtained from 7 patients having transurethral resections or radical prostatectomies. The tissue was fixed in 3% glutaraldehyde- 0.1 M cacodylate buffer (pH 7.4) for 15 min, sectioned at 50 μm on a Sorvall TC-2 tissue sectioner, refixed for a total of 2 hr, and rinsed overnight in 0.1 M cacodylate buffer (pH 7.4)-7.5% sucrose.


1951 ◽  
Vol 17 (3) ◽  
pp. 460-461
Keyword(s):  

2007 ◽  
Vol 177 (4S) ◽  
pp. 121-121
Author(s):  
Luciano A. Favorito ◽  
Francisco J.B. Sampaio
Keyword(s):  

2007 ◽  
Vol 177 (4S) ◽  
pp. 568-568 ◽  
Author(s):  
Jack Groskopf ◽  
Ina L. Deras ◽  
Amy Blase ◽  
Sheila M.J. Aubin ◽  
Seongjoon Koo ◽  
...  

2007 ◽  
Vol 177 (4S) ◽  
pp. 1-2
Author(s):  
Adam W. Levinson ◽  
Richard E. Link ◽  
Lynda Z. Mettee ◽  
Soroush Rais-Bahrami ◽  
Devesh Agarwal ◽  
...  

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