scholarly journals INDUKSI MUTASI DAN SELEKSI IN VITRO TANAMAN GANDUM (Triticum aestivum L.)

2016 ◽  
Vol 3 (2) ◽  
pp. 48
Author(s):  
Laela Sari ◽  
Agus Purwito ◽  
Didy Soepandi ◽  
Ragapadmi Purnamaningsih ◽  
Enny Sudarmonowati
Keyword(s):  
Triticum Aestivum ◽  
In Vitro Selection ◽  
Triticum Aestivum L ◽  
Callus Growth ◽  
Wheat Crop ◽  
Soaking Time ◽  
Induced Mutation ◽  
Callus Production ◽  
Selection Of

INDUCTION MUTATION AND SELECTION OF IN VITRO PLANT OF WHEAT (Triticum aestivum L.)The goal of this research was to produce wheat crop which is tolerant to lowland condition.Six varieties were used, Dewata, Selayar, Alibey, Oasis, Rabe and HP1744. This research consisted of 4 stages: production of the best callus on MS medium containing 3 g/L 2.4-D, induced mutation of embryogenic callus using EMS, in vitro selection of callus at temperature of 27–35°C, and callus regeneration. The best result for callus production was 76% for Dewata and 70% for Selayar varieties. Higher concentration of EMS and longer soaking time decreased the percentage of callus growth. LC50 for Dewata was 0.3% EMS at 30 minutes and that for Selayar was 0.1% EMS at 60 minutes. The higher the temperature was, the lower was the adaptation tolerant of the plants, and callus growth was inhibited. At the highest temperature (35°C) the callus did not grow at all.Keywords: Induced mutation, Triticum aestivum, EMS, in vitro selection, callusABSTRAKTujuan penelitian ini adalah untuk merakit tanaman gandum yang toleran pada dataran rendah. Varietas yang digunakan ada 6 yaitu Dewata, Selayar, Alibey, Oasis, Rabe dan HP-1744. Penelitian terdiri atas empat tahap yaitu induksi pembentukan kalus terbaik menggunakan media MS + 3 g/L 2,4-D (dipilih dua varietas yang terbaik), induksi mutasi kalus embriogenik menggunakan EMS, seleksi kalus in vitro pada suhu 27–35°C, dan regenerasi. Hasil induksi kalus terbaik terdapat pada varietas Dewata sebesar 76% dan Selayar sebesar 70%. Semakin tinggi konsentrasi EMS dan semakin lama waktu perendaman yang digunakan maka semakin menurun persentase pertumbuhan kalus. LC50 varietas Dewata adalah EMS 0,3% waktu 30 menit sedangkan LC50 varietas Selayar adalah EMS 0,1% waktu 60 menit.Semakin tinggi suhunya maka semakin berkurang toleran adaptasi tanaman tersebut, dan pertumbuhan kalus semakin sedikit. Bahkan pada suhu tertinggi yaitu suhu 35°C tidak ada pertumbuhan kalus sama sekali.Kata Kunci: Induksi mutasi, Triticum aestivum, EMS, seleksi in vitro, kalus

scholarly journals Influence of Sowing Dates and Varieties on the Biological Yield of Wheat Crop (Triticum aestivum L.)

2021 ◽  
pp. 9-15
Author(s):  
Sediq Omar Roshan ◽  
Rahmatullah Hashimi ◽  
Ghulam Habib Noori
Keyword(s):  
Triticum Aestivum ◽  
Triticum Aestivum L ◽  
The Other ◽  
Wheat Crop ◽  
Lower Yield ◽  
Wheat Varieties ◽  
Sowing Dates ◽  
Biological Yield ◽  
Selection Of ◽  
Late Sowing

A field experiment was carried out in Dar-ul-Aman Research Farm, Kabul Afghanistan in 2017, to study the influence of sowing dates and varieties on the biological yield of wheat crop (Triticum aestivum L.). The experiment was laid out in RCBD with a split-plot arrangement having three replications. Wheat was sown in different sowing dates i.e., October 24th, November 2nd, 12th, and 22nd in main plots, whereas five wheat varieties (Chounth # 1-2010, Moqawim-09, Shisham Bagh-08, Dar-ul-Aman-07 and Solh 2002) were in sub plots. November 2nd planted wheat had a highest biological yield of (6642.1 kg ha-1) followed by October 24th and November 12th which gave (6576.5) and (5711.0 kg ha-1) respectively. The lowest biological yield of (2975.1 kg ha-1) was given by the wheat varieties sown on November 22nd. Among the all-wheat varieties, Solh 2002, had significantly higher biological yield of (6263.7 kg ha-1) followed by Shisham Bagh-08 with biological yield of (5571.8 kg ha-1). However, Moqawim-09 had lowest biological yield of (4976.8 kg ha-1) compare to other varieties. While, evaluating performance of different wheat varieties on different sowing dates, Solh 2002, resulted in maximum biological yield (7476.2 kg ha-1) on November 2nd followed by Dar-ul-Aman-07 (6933.3 kg ha-1) and Shisham Bagh-08 (6860.0 kg ha-1) on November 2nd and October 24th respectively. On the other hand, Dar-ul-Aman-07 had lowest biological yield among the varieties (2015.5 kg ha-1) on November 22nd, it shows that, Dar-ul-Aman-07 had highest biological yield on early planting but lower yield on late sowing dates. The study shows that delay in wheat planting or selection of other varieties rather than Solh 2002 and Dar-ul-Aman-07 reduces the biological yield of the crop.

The Effects of Shock Vancomycin Concentrations on the Formation of Heteroresistance in Staphylococcus aureus

2020 ◽  
Vol 65 (9-10) ◽  
pp. 3-7
Author(s):  
V. V. Gostev ◽  
Yu. V. Sopova ◽  
O. S. Kalinogorskaya ◽  
M. E. Velizhanina ◽  
I. V. Lazareva ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
In Vitro Selection ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
High Concentration ◽  
Short Term ◽  
High Concentrations ◽  
Selection Of ◽  
Selection Of Resistance ◽  
Test Cultures

Glycopeptides are the basis of the treatment of infections caused by MRSA (Methicillin-Resistant Staphylococcus aureus). Previously, it was demonstrated that antibiotic tolerant phenotypes are formed during selection of resistance under the influence of high concentrations of antibiotics. The present study uses a similar in vitro selection model with vancomycin. Clinical isolates of MRSA belonging to genetic lines ST8 and ST239, as well as the MSSA (ATCC29213) strain, were included in the experiment. Test isolates were incubated for five hours in a medium with a high concentration of vancomycin (50 μg/ml). Test cultures were grown on the medium without antibiotic for 18 hours after each exposure. A total of ten exposure cycles were performed. Vancomycin was characterized by bacteriostatic action; the proportion of surviving cells after exposure was 70–100%. After selection, there was a slight increase in the MIC to vancomycin (MIC 2 μg/ml), teicoplanin (MIC 1.5–3 μg/ml) and daptomycin (MIC 0.25–2 μg/ml). According to the results of PAP analysis, all strains showed an increase in the area under curve depending on the concentration of vancomycin after selection, while a heteroresistant phenotype (with PAP/AUC 0.9) was detected in three isolates. All isolates showed walK mutations (T188S, D235N, E261V, V380I, and G223D). Exposure to short-term shock concentrations of vancomycin promotes the formation of heteroresistance in both MRSA and MSSA. Formation of VISA phenotypes is possible during therapy with vancomycin.

Wheat Germ Cell-Free Translation System as a Tool for In vitro Selection of Functional Proteins

2002 ◽  
Vol 5 (6) ◽  
pp. 473-480
Author(s):  
Bentham Science Publisher A.N. Alexandrov ◽  
Bentham Science Publisher V.Yu. Alakhov ◽  
Bentham Science Publisher A.I. Miroshnikov
Keyword(s):  
Germ Cell ◽  
Wheat Germ ◽  
In Vitro Selection ◽  
Translation System ◽  
Free Translation ◽  
Selection Of

In vitro Selection of Ligase Ribozymes Containing 2'-Amino Groups

2000 ◽  
Vol 15 (4) ◽  
pp. 297-308 ◽  
Author(s):  
NAOZUMI TERAMOTO ◽  
YUKIO IMANISHI ◽  
YOSHIHIRO ITO
Keyword(s):  
In Vitro Selection ◽  
Amino Groups ◽  
Selection Of

scholarly journals Back Cover: In Vitro Selection of a DNA Aptamer Targeting Degraded Protein Fragments for Biosensing (Angew. Chem. Int. Ed. 20/2020)

2020 ◽  
Vol 59 (20) ◽  
pp. 7968-7968
Author(s):  
Meng Liu ◽  
Jiayi Wang ◽  
Yangyang Chang ◽  
Qiang Zhang ◽  
Dingran Chang ◽  
...  
Keyword(s):  
In Vitro Selection ◽  
Dna Aptamer ◽  
Protein Fragments ◽  
Back Cover ◽  
Selection Of
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