scholarly journals Farklı sıcaklıkların Leptinotarsa decemlineata (Say.) (Coleoptera: Chrysomelidae)’nın beslenme miktarı üzerine etkisi

2021 ◽  
Vol 34 (2) ◽  
pp. 175-180
Author(s):  
Büşra Betül ÇINAR ◽  
Ali Kemal BİRGÜCÜ
Keyword(s):  
Leptinotarsa Decemlineata ◽  
Leptinotarsa Decemlineata Say

scholarly journals Biochemical characterisation of α-glucosidase and β-glucosidase in the alimentary canal of larval Leptinotarsa decemlineata SAY, 1824 (Coleoptera: Chrysomelidae)

2014 ◽  
Vol 83 (4) ◽  
pp. 281-294 ◽  
Author(s):  
Majid Kazzazi ◽  
Fahimeh Dehghanikhah ◽  
Hossein Madadi ◽  
Vahid Hossseininaveh
Keyword(s):  
Incubation Time ◽  
Leptinotarsa Decemlineata ◽  
Digestive Enzymes ◽  
Specific Activity ◽  
Inhibitory Effect ◽  
Maximum Activity ◽  
Pest Population ◽  
Biochemical Characterisation ◽  
Leptinotarsa Decemlineata Say ◽  
Environmentally Safe

ABSTRACT Host plant resistance is an environmentally safe method used for reducing a pest population. Basically, when developing resistant cultivars one needs to study the biochemical characteristics of the digestive enzymes in the insect’s midgut. In this study, the activities of α- and β-glucosidase were determined from Leptinotarsa decemlineata midgut using p-nitrophenyl-α-Dglucopyranoside and p-nitrophenyl-β-D-glucopyranoside as substrates respectively. The results showed that the specific activity of α- and β-glucosidase from 4th instar larvae midguts of L. decemlineata were 5.14 and 5.48 Umg-1 protein respectively. The activity of α-glucosidase was optimal at pH 4, whereas the maximum activity of β-glucosidase in the midgut of L. decemlineata occurred at pH 4-5.5. Both enzymes were stable at pH 3-8 over an incubation time of 8 hours. The respective activities of α- and β-glucosidase were at their highest at 45 °C and 50 °C, but they were not stable at 50 °C during an incubation time of 8 days. Furthermore, our data showed that MgCl2, Tris and urea have a moderate but SDS a severe inhibitory effect on enzyme activity. Biochemical characterisation revealed one and three bands of α- and β-glucosidase activities in the midgut of L. decemlineata respectively.

THE EFFECT OF WATER STRESS AND LEAFLET SIZE ON THE DENSITY OF TRICHOMES AND THE RESISTANCE TO COLORADO POTATO BEETLE LARVAE (LEPTINOTARSA DECEMLINEATA [SAY]) IN SOLANUM BERTHAULTII HAWKES

1990 ◽  
Vol 122 (6) ◽  
pp. 1141-1147 ◽  
Author(s):  
Yvan Pelletier
Keyword(s):  
Water Stress ◽  
Colorado Potato Beetle ◽  
Leptinotarsa Decemlineata ◽  
Solanum Berthaultii ◽  
Abaxial Surface ◽  
First Instar ◽  
Potato Beetle ◽  
Resistance To Insects ◽  
Leptinotarsa Decemlineata Say ◽  
Growing Conditions

AbstractSolanum berthaultii Hawkes bears trichomes reportedly providing resistance to insects. Results indicated that the density of types A and B trichomes was linearly related to the inverse of the leaflet area. The number of trichomes on the adaxial and on the abaxial surface of terminal leaflets was not affected by two levels of water stress or by growing conditions in the greenhouse or the field. The leaflets of the water-stressed plants were generally smaller and, consequently, the density of trichomes higher.The survival of first-instar Colorado potato beetle larvae was similar on S. berthaultii grown under varying drought conditions and on leaves from field-grown plants bearing different densities of trichomes.

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