Isolation and Characterization of Pseudomonas Fluorescens and It's Siderophores Production

2017 ◽  
Vol 4 (3) ◽  
pp. 44
Author(s):  
S. Nithyapriya ◽  
S. Lalitha
Keyword(s):  
Pseudomonas Fluorescens ◽  
Isolation And Characterization

Isolation and characterization of two lytic cold-active bacteriophages infecting Pseudomonas fluorescens from the Napahai plateau wetland

2018 ◽  
Vol 64 (3) ◽  
pp. 183-190 ◽  
Author(s):  
Yingying Xiang ◽  
Shuang Wang ◽  
Jiankai Li ◽  
Yunlin Wei ◽  
Qi Zhang ◽  
...  
Keyword(s):  
Pseudomonas Fluorescens ◽  
Flood Control ◽  
Long Tail ◽  
Ph Values ◽  
Plateau Wetland ◽  
Isolation And Characterization ◽  
Adaptation Mechanisms ◽  
The Earth ◽  
Cold Active

As the “kidneys of the Earth”, wetlands play important roles as biodiversity reservoirs, in water purification, and in flood control. In this study, 2 lytic cold-active bacteriophages, named VW-6S and VW-6B, infecting Pseudomonas fluorescens W-6 cells from the Napahai plateau wetland in China were isolated and characterized. Electron microscopy showed that both VW-6S and VW-6B had an icosahedral head (66.7 and 61.1 nm, respectively) and a long tail (8.3 nm width × 233.3 nm length and 11.1 nm width × 166.7 nm length, respectively). The bacteriophages VW-6S and VW-6B were classified as Siphoviridae and had an approximate genome size of 30–40 kb. The latent and burst periods of VW-6S were 60 and 30 min, whereas those of VW-6B were 30 and 30 min, respectively. The optimal pH values for the bacteriophages VW-6S and VW-6B were 8.0 and 10.0, respectively, and their activity decreased rapidly at temperatures higher than 60 °C. These cold-active bacteriophages provide good materials for further study of cold-adaptation mechanisms and interaction with the host P. fluorescens.

ChemInform Abstract: Isolation and Characterization of Antimicrobial Cyclic Dipeptides from Pseudomonas fluorescens and Their Efficacy on Sorghum Grain Mold Fungi.

ChemInform ◽  
2014 ◽  
Vol 45 (16) ◽  
pp. no-no
Author(s):  
Ahil Sajeli Begum ◽  
Shaik Ameer Basha ◽  
Govardhanam Raghavendra ◽  
Mallela Venkata Nagesh Kumar ◽  
Yukthi Singh ◽  
...  
Keyword(s):  
Pseudomonas Fluorescens ◽  
Cyclic Dipeptides ◽  
Isolation And Characterization ◽  
Grain Mold ◽  
Sorghum Grain

Isolation and characterization of wetland VSW-3, a novel lytic cold-active bacteriophage of Pseudomonas fluorescens

2017 ◽  
Vol 63 (2) ◽  
pp. 110-118 ◽  
Author(s):  
Kunhao Qin ◽  
Xiuling Ji ◽  
Chunjing Zhang ◽  
Yafang Ding ◽  
Anxiu Kuang ◽  
...  
Keyword(s):  
Pseudomonas Fluorescens ◽  
Burst Size ◽  
Isolation And Characterization ◽  
A Genome ◽  
Cold Active ◽  
Average Burst Size ◽  
20 Nm ◽  
High Degree ◽  
Thermal Stability Of

Wetlands are often called the “kidneys of the Earth” and contribute substantially to environmental improvement. Pseudomonas fluorescens is a major contaminant of milk products and causes the spoilage of refrigerated foods and fresh poultry. In this study, we isolated and characterized a lytic cold-active bacteriophage named VSW-3 together with P. fluorescens SW-3 cells from the Napahai wetland in China. Electron microscopy showed that VSW-3 had an icosahedral head (56 nm) and a tapering tail (20 nm × 12 nm) and a genome size of approximate 40 kb. On the basis of the top-scoring hits in the BLASTP analysis, VSW-3 showed a high degree of module similarity to the Pseudomonas phages Andromeda and Bf7. The latent and burst periods were 45 and 20 min, respectively, with an average burst size of 90 phage particles per infected cell. The pH and thermal stability of VSW-3 were also explored. The optimal pH was found to be 7.0 and the activity decreased rapidly when the temperature exceeded 60 °C. VSW-3 is a cold-active bacteriophage, hence, it is important to research its ability to prevent product contamination caused by P. fluorescens and to characterize its relationship with its host P. fluorescens in the future.

scholarly journals Isolation and characterization of an aluminum-sensitive mutant of Pseudomonas fluorescens

2006 ◽  
Vol 152 (2) ◽  
pp. 287-292
Author(s):  
Vasu D Appanna ◽  
Michael Rauh ◽  
Robert Hamel ◽  
Eric Gauthier
Keyword(s):  
Pseudomonas Fluorescens ◽  
Sensitive Mutant ◽  
Isolation And Characterization
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