scholarly journals False-positive findings of hepatitis C virus antibody in alcoholic hepatits checked by use of the polymerase chain reaction to amplify HCV-RNA.

Kanzo ◽  
1990 ◽  
Vol 31 (11) ◽  
pp. 1365-1366
Author(s):  
Shuhei NISHIGUCHI ◽  
Tetsuo KUROKI ◽  
Tadashi UEDA ◽  
Tadashi TAKEDA ◽  
Shinya NAKAJIMA ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
False Positive ◽  
Hcv Rna ◽  
Virus Antibody ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Hepatitis C Virus Antibody

scholarly journals Hepatitis C virus serum markers and liver disease in children with leukemia during and after chemotherapy

Blood ◽  
1993 ◽  
Vol 82 (8) ◽  
pp. 2564-2567 ◽  
Author(s):  
A Locasciulli ◽  
D Cavalletto ◽  
P Pontisso ◽  
L Cavalletto ◽  
E Scovena ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis C Virus ◽  
Liver Disease ◽  
Hepatitis C ◽  
Second Generation ◽  
Serum Markers ◽  
Hcv Infection ◽  
Hcv Rna ◽  
Chain Reaction ◽  
Polymerase Chain

The pattern of hepatitis C virus (HCV) serum markers and liver disease was investigated in 11 leukemic children showing anti-HCV reactivity at least once during long-term observation to define the role of HCV infection and the behavior of HCV serologic markers in this patient cohort. Antibodies to HCV by first- and second-generation enzyme-linked immunosorbent assay (ELISA) and by second-generation (four antigens) recombinant immunoblotting assay (RIBA) and HCV-RNA by nested polymerase chain reaction (PCR) were serially examined in serum. Liver disease was defined according to transaminase levels. Seven of 11 patients were found HCV-RNA positive during chemotherapy and after blood transfusion, 3 of 11 became viremic during follow-up, and 1 of 11 was always HCV-RNA negative. Seroconversion to anti-HCV positivity by second-generation ELISA occurred in all the HCV-RNA positive children either during or after chemotherapy. Alanine aminotransferase (ALT) levels were elevated in all the HCV-RNA positive patients during antileukemic treatment and normalized in seven of them after therapy withdrawal, despite persisting viremia. These results indicate that HCV- RNA testing by polymerase chain reaction is required to correctly identify HCV infection in patients with leukemia while on chemotherapy. Viremia did not correlate with ALT levels and anti-HCV patterns.

Evaluation of a Disinfection Procedure for Hysteroscopes Contaminated by Hepatitis C Virus

1999 ◽  
Vol 20 (6) ◽  
pp. 434-436 ◽  
Author(s):  
Catherine Sartor ◽  
Remi Nicolas Charrel ◽  
Xavier de Lamballerie ◽  
Roland Sambuc ◽  
Philippe De Micco ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Water Channel ◽  
Hcv Rna ◽  
Chain Reaction ◽  
Disinfection Procedure ◽  
Hcv Transmission ◽  
Polymerase Chain

AbstractWe assessed the ability of a standard disinfection procedure to eliminate hepatitis C virus (HCV) from the air-water channel of hysteroscopes. The residual HCV RNA remaining after the disinfection procedure was measured by polymerase chain reaction. When correctly applied to hysteroscopes, the standard disinfection procedure was sufficient to eliminate the risk of HCV transmission.

scholarly journals Hepatitis C virus serum markers and liver disease in children with leukemia during and after chemotherapy

Blood ◽  
1993 ◽  
Vol 82 (8) ◽  
pp. 2564-2567 ◽  
Author(s):  
A Locasciulli ◽  
D Cavalletto ◽  
P Pontisso ◽  
L Cavalletto ◽  
E Scovena ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Hepatitis C Virus ◽  
Liver Disease ◽  
Hepatitis C ◽  
Second Generation ◽  
Serum Markers ◽  
Hcv Infection ◽  
Hcv Rna ◽  
Chain Reaction ◽  
Polymerase Chain

Abstract The pattern of hepatitis C virus (HCV) serum markers and liver disease was investigated in 11 leukemic children showing anti-HCV reactivity at least once during long-term observation to define the role of HCV infection and the behavior of HCV serologic markers in this patient cohort. Antibodies to HCV by first- and second-generation enzyme-linked immunosorbent assay (ELISA) and by second-generation (four antigens) recombinant immunoblotting assay (RIBA) and HCV-RNA by nested polymerase chain reaction (PCR) were serially examined in serum. Liver disease was defined according to transaminase levels. Seven of 11 patients were found HCV-RNA positive during chemotherapy and after blood transfusion, 3 of 11 became viremic during follow-up, and 1 of 11 was always HCV-RNA negative. Seroconversion to anti-HCV positivity by second-generation ELISA occurred in all the HCV-RNA positive children either during or after chemotherapy. Alanine aminotransferase (ALT) levels were elevated in all the HCV-RNA positive patients during antileukemic treatment and normalized in seven of them after therapy withdrawal, despite persisting viremia. These results indicate that HCV- RNA testing by polymerase chain reaction is required to correctly identify HCV infection in patients with leukemia while on chemotherapy. Viremia did not correlate with ALT levels and anti-HCV patterns.

scholarly journals Evaluation of Nitric Oxide (NO) Levels in Hepatitis C Virus (HCV) Infection: Relationship to Schistosomiasis and Liver Cirrhosis among Egyptian Patients

2002 ◽  
Vol 18 (3) ◽  
pp. 137-142 ◽  
Author(s):  
Mahmoud Ismail Hassan ◽  
Samar Kamal Kassim ◽  
Hebatalla Said Ali ◽  
El-Dieb Abd ElSattar Sayed ◽  
Ali Khalifa
Keyword(s):  
Nitric Oxide ◽  
Polymerase Chain Reaction ◽  
Liver Cirrhosis ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Hcv Rna ◽  
Chain Reaction ◽  
Virus Load ◽  
Egyptian Patients ◽  
Polymerase Chain

Nitric oxide (NO), a recently discovered free radical, is overproduced in liver cirrhosis. Hepatitis C virus (HCV) might increase NO levels via increased inducible NO synthase (iNOS). This work was carried out to study the effect of HCV-induced liver cirrhosis on NO levels among Egyptian patients. The study included 46 patients with liver cirrhosis, and 30 healthy individuals of matched age and sex. NO levels determined as the stable endproduct nitrate, showed a statistically significant increase among patients compared to the control group (P< 0.001). Furthermore, NO levels increased proportionally with the severity of liver cirrhosis as assessed by Child’s classification (P< 0.05). Moreover, schistosomial infection enhanced NO levels in cirrhotic patients with HCV infection compared to non-bilharzial patients (P< 0.001). Polymerase chain reaction (PCR) and branched DNA assays were used for detection of HCV RNA positivity, and measurement of the virus load, respectively. Both showed a positive correlation with the NO levels (P< 0.001). At a nitrate cutoff value of 70μmol/L, the sensitivity and specificity were 83.0% and 37.0% respectively. Chi square analysis showed a significant correlation between ALT levels and both HCV RNA positivity by polymerase chain reaction (PCR) (P< 0.02), and virus load (P< 0.05). Interestingly enough, there was a significant positive correlation between HCV RNA and schistosomal antibody titer as measured by hemaglutination inhibition assay (HAI) (P< 0.05). The data presented in this report indicated an association between NO levels and the development and progression of liver cirrhosis. Furthermore, the findings obtained from this study demonstrated that schistomiasis is an important risk factor involved in enhancement of NO levels and virus replication. The latter may aggravate liver cell injury and hence the development of cirrhosis.

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