Development of Three Additional Culex Species-Specific Polymerase Chain Reaction Primers and Their Application in West Nile Virus Surveillance in Canada

2010 ◽  
Vol 26 (1) ◽  
pp. 37-42 ◽  
Author(s):  
Mahmood Iranpour ◽  
L. Robbin Lindsay ◽  
Antonia Dibernardo
Keyword(s):  
Polymerase Chain Reaction ◽  
West Nile Virus ◽  
Chain Reaction ◽  
West Nile ◽  
Specific Polymerase Chain Reaction ◽  
Virus Surveillance ◽  
Polymerase Chain ◽  
Species Specific ◽  
Polymerase Chain Reaction Primers

Routine identification of four sympatric species of calanoid copepods Pseudocalanus spp. in the Atlantic Arctic using a species-specific polymerase chain reaction

2020 ◽  
Vol 48 (1) ◽  
pp. 62-72
Author(s):  
E. A. Ershova
Keyword(s):  
Polymerase Chain Reaction ◽  
Life Cycles ◽  
The Arctic ◽  
Relative Distribution ◽  
Chain Reaction ◽  
Specific Polymerase Chain Reaction ◽  
Routine Identification ◽  
Polymerase Chain ◽  
Species Specific ◽  
Plankton Communities

Сalanoid copepods of the genus Pseudocalanus play an important role in the plankton communities of the Arctic and boreal seas, often dominating in numbers and constituting a significant proportion of the biomass of zooplankton. Despite their high presence and significance in the shelf plankton communities, species-specific studies of the biology of these are significantly hampered by extremely small morphological differences between them, especially at the juvenile stages, at which they are virtually indistinguishable. In this paper, we describe a new, routine and low-cost molecular method for identifying all Pseudocalanus species found in the Atlantic sector of the Arctic: the Arctic P. acuspes, P. minutus and the boreal P. moultoni and P. elongatus, and apply it to describe the relative distribution of these species in four locations of the Arctic and sub-Arctic. With this method, species-specific polymerase chain reaction (ssPCR), mass identification of individuals of any developmental stage, including nauplii, is possible. This method can serve as an excellent tool for studying the species-specific biology of this group, describing their life cycles, as well as monitoring changes in Arctic marine ecosystems under the influence of changing climate.

scholarly journals Mosquito Surveillance and Polymerase Chain Reaction Detection of West Nile Virus, New York State

2001 ◽  
Vol 7 (4) ◽  
pp. 643-649 ◽  
Author(s):  
Dennis J. White ◽  
Laura D. Kramer ◽  
P. Bryon Backenson ◽  
Gary Lukacik ◽  
Geraldine Johnson ◽  
...  
Keyword(s):  
New York ◽  
Polymerase Chain Reaction ◽  
West Nile Virus ◽  
New York State ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
West Nile ◽  
Mosquito Surveillance ◽  
York State ◽  
Polymerase Chain
Sign in / Sign up

Export Citation Format

Share Document