scholarly journals Detection Contaminant DNA of a Pig on a Sample Meat Mills in Some City Markets Bengkulu, Using a Genetic Marker DNA Mitochondria Cytochrome B

Author(s):  
Choirul Muslim ◽  
Iin Isnaini ◽  
Sipriyadi
Keyword(s):  
Author(s):  
A. Ni'mah ◽  
Y. Kartikasari ◽  
A. D. Pratama ◽  
L. R. Kartikasari ◽  
B. S. Hertanto ◽  
...  

2017 ◽  
Vol 42 (3) ◽  
pp. 135 ◽  
Author(s):  
D. A. Lestari ◽  
S. Sutopo ◽  
E. Kurnianto

The objectives of study were to identify and assess the amino acid diversity of Cytochrome b (Cyt b) gene, genetic marker and characteristic of specific amino acid in Kacang and Ettawa Grade goat. Nineteen heads of Kacang goat (KG) and twelve heads of Ettawa Grade goat (EG) were purposively sampled. The genomic DNA was isolated by Genomic DNA Mini Kit (Geneaid) and amplified Cyt b using PCR method with CytbCapF and CytbCapR primers and was sequenced. The results showed that there were two specific amino acids that distinguish KG and EG goat with C. hircus and C. aegagrus and four specific amino acids that distinguish KG and EG goat with C. falconeri, but there were no specific amino acids can be used as a genetic marker to distinguish between Kacang and EG goat. In conclusion, specific amino acids in Cyt b gene can be used as a genetic marker among KG and EG goat with 3 goat others comparator.


2016 ◽  
Vol 19 (3) ◽  
pp. 122-135 ◽  
Author(s):  
Rony Marsyal Kunda ◽  
Niken Satuti Nur Handayani ◽  
Hery Wijayanto ◽  
Rini Widayanti

Author(s):  
Jean-François Agnèse ◽  
Laurent Chirio ◽  
Olivier Legros ◽  
Richard Oslisly ◽  
Hervé Mvé Bhé

During a survey of the fishes in the region of the Wonga-Wongué Presidential Reserve, 14 new populations of the subgenus Chromaphyosemion Myers, 1924 were found. These observations extend the previously known distribution range of the subgenus 120 kilometres southward. None of these populations could be related to any described species. Based on the colouration of the males and females, together with a genetic marker (mitochondrial DNA cytochrome b sequences), the populations studied are grouped into six new species which are described in this article, all close to Aphyosemion alpha Huber, 1998 with which they share the presence of a black alpha-shaped mark on the pre- and post-opercular region. The group composed of A. alpha and the six new species is referred to here as the A. alpha species group. All the new species, A. aurantiacum Chirio, Legros & Agnèse sp. nov., A. barakoniense Chirio, Legros & Agnèse sp. nov., A. flammulatum Chirio, Legros & Agnèse sp. nov., A. flavocyaneum Chirio, Legros & Agnèse sp. nov., A. pusillum Chirio, Legros & Agnèse sp. nov. and A. rubrogaster Chirio, Legros & Agnèse sp. nov., are further unambiguously diagnosed by unique combinations of colour patterns, making it possible to generate an identification key for the A. alpha species group. It is likely that the coastal dunes of Wonga-Wongué that form a sandy relief, could have led to the fragmentation and then isolation of the hydrographical networks that flow into the Atlantic Ocean, making possible a significant number of allopatric speciations.


ZooKeys ◽  
2015 ◽  
Vol 478 ◽  
pp. 27-43 ◽  
Author(s):  
Ernieenor Faraliana Che Lah ◽  
Salmah Yaakop ◽  
Mariana Ahamad ◽  
Shukor Md Nor

Author(s):  
J. A. Clarke ◽  
D. N. Landon ◽  
P. R. Ward

Intra-mitochondrial crystals have been noted in muscle biopsies from patients in a wide variety of diseased states. As far as we are aware, none of these crystals have been subjected to detailed crystallographic analysis. Recently, similar crystals were observed in a biopsy from a patient with a mitochondrial myopathy, characterised by a deficiency in reducible cytochrome b (Morgan-Hughes, J. A., Darveniza, P., Kahn, S. N., Landon, D. N., Sherratt, R. M., Land, J. M. and Clark, J. B., 1977, Brain, In Press). Aldehyde-fixed, osmicated resin imbedded material was examined using Siemens, JEOL and Phillips electron microscopes with goniometer specimen stages. The crystals generally lay between the outer and inner mitochondrial membranes and measured 1 - 3 μm in length and 0.1 - 0.3 μm in width. Characteristically, these crystals revealed specific periodicities.


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