scholarly journals Effect of different seed sludge on start-up of EGSB reactor and Microbial community analysis by next generation sequencing

2019 ◽  
Author(s):  
Runhua Liao ◽  
Zhongyan Chen ◽  
Jie Du ◽  
Yueming Li ◽  
Huijuan Shen
Keyword(s):  
Microbial Community ◽  
Next Generation Sequencing ◽  
Community Analysis ◽  
Microbial Community Analysis ◽  
Next Generation ◽  
Start Up ◽  
Seed Sludge ◽  
Generation Sequencing

Microbial community analysis of anaerobic granules in phenol-degrading UASB by next generation sequencing

2016 ◽  
Vol 112 ◽  
pp. 241-248 ◽  
Author(s):  
Jeong-Geol Na ◽  
Mo-Kwon Lee ◽  
Yeo-Myeong Yun ◽  
Chungman Moon ◽  
Mi-Sun Kim ◽  
...  
Keyword(s):  
Microbial Community ◽  
Next Generation Sequencing ◽  
Community Analysis ◽  
Microbial Community Analysis ◽  
Next Generation ◽  
Generation Sequencing

scholarly journals NEXT GENERATION SEQUENCING AND MICROBIAL COMMUNITY ASSOCIATED WITH EUKARYOTES INCLUDING PARASITIC HELIMINTHS: A REVIEW ARTICLE.

2017 ◽  
Vol 5 (3) ◽  
pp. 1003-1022
Author(s):  
SaeedEl Ashram ◽  
◽  
IbrahimAl Nasr ◽  
Rashid mehmood ◽  
Min Hu ◽  
...  
Keyword(s):  
Microbial Community ◽  
Next Generation Sequencing ◽  
Review Article ◽  
Next Generation ◽  
Generation Sequencing

scholarly journals Mercury contamination imposes structural shift on the microbial community of an agricultural soil

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Lateef Babatunde Salam ◽  
Halima Shomope ◽  
Zainab Ummi ◽  
Fatima Bukar
Keyword(s):  
Heavy Metals ◽  
Microbial Community ◽  
Next Generation Sequencing ◽  
Agricultural Soil ◽  
Mercury Contamination ◽  
Next Generation ◽  
Mercury Stress ◽  
Mercury Detoxification ◽  
Generation Sequencing ◽  
Detoxification Process

Abstract Background The purpose of this study is to use shotgun next-generation sequencing to unravel the microbial community structure of an agricultural soil, decipher the effects of mercury contamination on the structure of the microbial community and the soil physicochemistry and heavy metals content. Results The soil physicochemistry after mercury contamination revealed a shift in soil pH from neutral (6.99 ± 0.001) to acidic (5.96 ± 0.25), a decline in moisture content to < 4 %, and a significant decrease in the concentrations of all the macronutrients and the total organic matter. Significant decrease in all the heavy metals detected in the agricultural soil was also observed in mercury inundated SL3 microcosm. Structural analysis of the metagenomes of SL1 (agricultural soil) and SL3 (mercury-contaminated agricultural soil) using Illumina shotgun next-generation sequencing revealed the loss due to mercury contamination of 54.75 % of the microbial community consisting of an archaeal domain, 11 phyla, 12 classes, 24 orders, 36 families, 59 genera, and 86 species. The dominant phylum, class, genus, and species in SL1 metagenome are Proteobacteria, Bacilli, Staphylococcus, and Sphingobacterium sp. 21; while in SL3 metagenome, Proteobacteria, Alphaproteobacteria, Singulisphaera, and Singulisphaera acidiphila were preponderant. Mercury contamination resulted in a massive upscale in the population of members of the phylum Planctomycetes and the genera Singulisphaera, Brevundimonas, Sanguibacter, Exiguobacterium, Desulfobacca, and Proteus in SL3 metagenome while it causes massive decline in the population of genera Staphylococcus and Brachybacterium. Conclusions This study revealed that mercury contamination of the agricultural soil imposed selective pressure on the members of the microbial community, which negatively impact on their population, alter soil physicochemistry, and enriched sizable numbers of members of the community that are well adapted to mercury stress. It also reveals members of microbial community hitherto not reported to be important in mercury detoxification process.

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