scholarly journals Molecular characterization of field isolates of Avian Infectious Laryngeotracheitis Virus from different farms in Iraq

2017 ◽  
Vol 40 (2) ◽  
pp. 31-35
Author(s):  
Aida Bara Allawe
Keyword(s):  
Polymerase Chain Reaction ◽  
Positive Result ◽  
Real Time ◽  
Virus Detection ◽  
Chain Reaction ◽  
Glycoprotein G ◽  
Polymerase Chain ◽  
Labeled Probe ◽  
First Time

     This study was conducted to detect virulent isolates of avian infectious laryngeotracheitis virus in Iraq by Real Time-Polymerase Chain Reaction with the amplification of glycoprotein G gene which is responsible for virulence of the virus. Seventy samples (larynx and trachea) were collected from different farms in Iraq to investigate presence of avian infectious laryngeotracheitis virus (detection of virulent isolates from other vaccine strains). Five samples out of seventy samples were virulent isolates (positive result) by using Real Time-Polymerase Chain Reaction utilizing flurescein amidite labeled probe specific for detection of isolates that have G gene (by amplification of G gene) for the first time in Iraq. These virulent isolates were negative by using Real Time-Polymerase Chain Reaction utilizing Quasar-labeled probe specific for the detection of attenuated isolates that lack G gene and targeted a region within glycoprotein J downstream from the sequence of glycoprotein G.

Detection of a few DNA copies by real-time electrochemical polymerase chain reaction

The Analyst ◽  
2017 ◽  
Vol 142 (18) ◽  
pp. 3432-3440 ◽  
Author(s):  
M. Moreau ◽  
S. Delile ◽  
A. Sharma ◽  
C. Fave ◽  
A. Perrier ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Current Work ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
First Time ◽  
Accurate Quantification

In the current work, accurate quantification over 10 to 108 DNA copies has been successfully achieved for the first time by real-time electrochemical PCR.

Characterization of GSTM3 polymorphism by real-time polymerase chain reaction with LightCycler

2004 ◽  
Vol 330 (1) ◽  
pp. 175-177
Author(s):  
Paola Mozzoni ◽  
Giuseppe De Palma ◽  
Eleonora Scotti ◽  
Marzia Capelletti ◽  
Antonio Mutti
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Chain Reaction ◽  
Polymerase Chain

Characterization of high-capacity adenovirus production by the quantitative real-time polymerase chain reaction: a comparative study of different titration methods

2008 ◽  
Vol 10 (10) ◽  
pp. 1092-1101 ◽  
Author(s):  
Julien Crettaz ◽  
Cristina Olague ◽  
Africa Vales ◽  
Igor Aurrekoetxea ◽  
Pedro Berraondo ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Comparative Study ◽  
Real Time ◽  
High Capacity ◽  
Chain Reaction ◽  
Polymerase Chain

Preparation and application of a BODIPY-labeled probe for a real-time polymerase chain reaction

2015 ◽  
Vol 41 (4) ◽  
pp. 451-453 ◽  
Author(s):  
E. V. Ilnitskaya ◽  
Yu. N. Kononevich ◽  
A. M. Muzafarov ◽  
S. A. Rzhevskiy ◽  
I. A. Shadrin ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Labeled Probe

scholarly journals Persistence of Dengue Virus (DENV-1, 2, 3,4) Transovarial-Transgenerational with Realtime Polymerase Chain Reaction (qPCR) in Ae. Aegypti and Ae. Albopictus (Diptera: Culicidae)

2021 ◽  
Vol 4 (8) ◽  
pp. 01-09
Author(s):  
Isna Hikmawati ◽  
Hendro Wahjono ◽  
Martini Martini ◽  
Edi Darmana ◽  
Soeharyo Hadisaputro ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dengue Virus ◽  
Real Time ◽  
Virus Detection ◽  
P Value ◽  
Chain Reaction ◽  
Laboratory Colony ◽  
Research Population ◽  
Quasi Experimental ◽  
Polymerase Chain

Ae. aegypti and Ae. albopictus have an important role in DHF transmission because they can simultaneously transmit the dengue virus vertically / transovarially or horizontally. This phenomenon indicates the persistence of the dengue virus by vectors. The aim of this research was to prove the persistence of the transovarial-transgenerational dengue virus (DENV-1,2,3,4) with real time polymerase chain reaction (qPCR) in Ae. aegypti and Ae. albopictus (Diptera: Culicidae). Quasi experimental design with intervention infects DENV 1-2-3-4 serotypes in Ae. aegypti and Ae. albopictus intratoracally. Research population Ae. aegypti and Ae. albopictus laboratory colony females. Dengue virus detection uses real-time polymerase chain reaction (qPCR). Transovarial detection by qPCR indicates detection of dengue virus in Ae. albopictus DENV-1 to progeny 1 (F1), DENV-2 and DENV-3 to F2, DENV-4 to F3. Next to Ae. aegypti DENV-1 to 1st progeny (F1), DENV-2 to F2, DENV-3 to F4 and DENV-4 to F3. there was no difference in MIR value (p value: 0.356) for the four serotypes in Ae. albopictus and Ae. aegypti. DENV-3 is the most persistent serotype in Ae. aegypti with 83.3% MIR and DENV-4 were the most persistent serotypes in Ae.albopictus with 100% MIR. The need to improve vector control models that focus not only on the main vector, but also other co-vectors.

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