Selective Potentiometric Sensors for the Determination of Quetiapine Fumarate in Pharmaceuticals and Spiked Human Urine

Two simple, rapid and accurate spectrophotometric procedures are proposed for the determination of quetiapine fumarate (QTF) in pharmaceuticals and in spiked human urine. The methods are based on charge transfer complexation reactions of free base form of the drug (quetiapine, QTP), as n-electron donor (D), with either p-chloranilic acid (p-CAA) (method A) or 2,3-dichloro-5,6-dicyanoquinone (DDQ) (method B) as ?-acceptors (A). The coloured charge transfer complexes produced exhibit absorption maxima at 520 and 540 nm, in method A and method B, respectively. The experimental conditions such as reagent concentration, reaction solvent and time have been carefully optimized to achieve the maximum sensitivity. Beer?s law is obeyed over the concentration ranges of 8.0 - 160 and 4.0 - 80.0 ?g ml-1, for method A and method B, respectively. The calculated molar absorptivity values are 1.77 ? 103 and 4.59 ? 103 l mol-1cm-1, respectively, for method A and method B. The Sandell sensitivity values, limits of detection (LOD) and quantification (LOQ) have also been reported. The stoichiometry of the reaction in both cases was accomplished adopting the limiting logarithmic method and was found to be 1: 2 (D: A). The accuracy and precision of the methods were evaluated on intra-day and inter-day basis. The proposed methods were successfully applied for the determination of QTF in pharmaceutical formulations and spiked human urine.

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Extractive Spectrophotometric Determination of Quetiapine Fumarate in Pharmaceuticals and Spiked Human Urine

Croatica Chemica Acta ◽

10.5562/cca1770 ◽

2012 ◽

pp. 9-17 ◽

Cited By ~ 5

Author(s):

Nagaraju Rajendraprasad ◽

Kanakapura Basavaiah ◽

Kanakapura Basavaiah Vinay

Keyword(s):

Spectrophotometric Determination ◽

Human Urine ◽

Quetiapine Fumarate ◽

Spiked Human Urine

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Sensitive Spectrofluorimetric Methods for Determination of Sitagliptin Phosphate, Dipeptidyl Peptidase-4 Inhibitor, in Pharmaceutical Tablets and Spiked Human Urine

Current Pharmaceutical Analysis ◽

10.2174/1573412913666170906164249 ◽

2018 ◽

Vol 14 (5) ◽

pp. 483-490 ◽

Cited By ~ 4

Author(s):

Marwa P. Bakr Ali ◽

Noha N. Atia

Keyword(s):

Human Urine ◽

Dipeptidyl Peptidase ◽

Dipeptidyl Peptidase 4 ◽

Pharmaceutical Tablets ◽

Dipeptidyl Peptidase 4 Inhibitor ◽

Spiked Human Urine

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Solvent bar microextraction combined with HPLC-DAD for simultaneous determination of diuretics in human urine and plasma samples

Current Pharmaceutical Biotechnology ◽

10.2174/1389201022666210222111943 ◽

2021 ◽

Vol 22 ◽

Author(s):

Nabil N. AL-Hashimi ◽

Amjad H. El-Sheikh ◽

Manal I. Alruwad ◽

Mohanad M. Odeh

Keyword(s):

Simultaneous Determination ◽

Human Urine ◽

High Performance ◽

Plasma Samples ◽

Satisfactory Precision ◽

Array Detection ◽

Spiked Human Urine ◽

Analytical Technology ◽

Solvent Bar Microextraction

Background: A simple and powerful microextraction procedure, the solvent bar microextraction (SBME), was used for the simultaneous determination of two diuretics, furosemide and spironolactone in human urine and plasma samples, using high-performance liquid chromatography coupled with diode array detection (HPLC-DAD). Methods: The appropriate amount (2 µL) of 1-octanol as an organic solvent confined within (2.5 cm) of a porous hollow fiber micro-tube, sealed at both ends was used for this procedure. The conditions for the SBME were optimized in water and the analytical performance were examined in spiked human urine and plasma samples. Results: The optimized method exhibited good linearity (R2 > 0.997) over the studied range of higher than 33 to 104 µg L-1 for furosemide and spironolactone in urine and plasma samples, illustrating a satisfactory precision level with RSD values between 2.1% and 9.1%. Discussion: The values of the limits of detection were found to be in the range of 6.39 to 9.67 µg L-1, and extraction recovery˃ 58.8% for both diuretics in urine and plasma samples. The applicability and effectiveness of the proposed method for the determination of furosemide and spironolactone in patient urine samples were tested. Conclusion: In comparison with reference methods, the attained results demonstrated that SBME combined with HPLC-DAD was proved to be simple, inexpensive, and promising analytical technology for the simultaneous determination of furosemide and spironolactone in urine and plasma samples.

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