Erratum: A Comparison of Buffered Tricaine Methanesulfonate (MS-222) and Isoeugenol Anesthesia for Caudal Fin Clipping in Zebrafish (Danio rerio)

2021 ◽  
Vol 60 (4) ◽  
pp. 491-491
Keyword(s):  
Danio Rerio ◽  
Caudal Fin ◽  
Tricaine Methanesulfonate

scholarly journals A Comparison of Buffered Tricaine Methanesulfonate (MS-222) and Isoeugenol Anesthesia for Caudal Fin Clipping in Zebrafish (Danio rerio)

2020 ◽  
Vol 59 (6) ◽  
pp. 732-736
Author(s):  
Gabrielle C Musk ◽  
Ben J Ezzy ◽  
Lisa M Kenchington ◽  
Wendy A Hopper ◽  
Lauren M Callahan
Keyword(s):  
Danio Rerio ◽  
Caudal Fin ◽  
Safety And Efficacy ◽  
Monitoring Time ◽  
Recovery From Anesthesia ◽  
Tricaine Methanesulfonate ◽  
Time Point

The purpose of this study was to compare the safety and efficacy of buffered tricaine methanesulfonate (MS-222) and isoeugenol for the anesthesia of zebrafish undergoing caudal fin clipping. Eighty 9 mo Danio rerio (AB strain) zebrafish were allocated to one of 2 equal groups: buffered MS-222 (168 mg/L, n = 40) or isoeugenol (20 mg/L, n = 40). The time to induction of anesthesia was significantly shorter in the isoeugenol group (141 ± 70 s) than in the MS-222 group (207 ± 103 s). The time to recovery from anesthesia was also shorter in the MS-222 group (373 ± 125 s) than in the isoeugenol group (491 ± 176 s). No obvious displays of distress or aversion to anesthesia were observed in either group. No difference was detected in the proportion of zebrafish that became anesthetized with either drug. One male zebrafish in the buffered MS-222 group was found dead at the 1-h post-procedural monitoring time point, but there was no difference between groups in the proportion of fish that survived anesthesia to the end of experiment. In conclusion, the safety and efficacy of buffered MS-222 (168 mg/L) and isoeugenol (20 mg/L) was similar for zebrafish undergoing anesthesia for caudal fin clipping.

Vitamin C supplementation improves growth performance and caudal fin regeneration in zebrafish ( Danio rerio )

2021 ◽  
Author(s):  
Pedro Luiz Pucci Figueiredo Carvalho ◽  
Pedro Henrique Ventura Almeida ◽  
William dos Santos Xavier ◽  
Igor Simões Tiagua Vicente ◽  
Matheus Gardim Guimarães ◽  
...  
Keyword(s):  
Vitamin C ◽  
Growth Performance ◽  
Danio Rerio ◽  
Caudal Fin ◽  
Fin Regeneration ◽  
Vitamin C Supplementation

scholarly journals The effects of Piper sarmentosum aqueous extracts on zebrafish (Danio rerio) embryos and caudal fin tissue regeneration

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Intan Zarina Zainol Abidin ◽  
Shazrul Fazry ◽  
Nur Hidayah Jamar ◽  
Herryawan Ryadi Ediwar Dyari ◽  
Zaidah Zainal Ariffin ◽  
...  
Keyword(s):  
Tissue Regeneration ◽  
Danio Rerio ◽  
Aqueous Extracts ◽  
Caudal Fin ◽  
Danio Rerio Embryos

Tricaine methanesulfonate (MS-222) on the spermatic quality of zebrafish, Danio rerio

Aquaculture ◽  
2021 ◽  
Vol 533 ◽  
pp. 736090
Author(s):  
Marina Zanin ◽  
Antonio Sergio Varela Junior ◽  
Izani Bonel Acosta ◽  
Stela Mari Meneghello Gheller ◽  
Etiane Zimermann ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Tricaine Methanesulfonate

Zebrafish (Danio rerio) as a Model for Understanding the Process of Caudal Fin Regeneration

Zebrafish ◽  
2020 ◽  
Vol 17 (6) ◽  
pp. 359-372
Author(s):  
Lina Lebedeva ◽  
Beibitgul Zhumabayeva ◽  
Tatyana Gebauer ◽  
Ilya Kisselev ◽  
Zaure Aitasheva
Keyword(s):  
Danio Rerio ◽  
Caudal Fin ◽  
Fin Regeneration

Propolis Can Improve Caudal Fin Regeneration in Zebrafish (Danio rerio) Induced by The Combined Administration of Alloxan and Glucose

Zebrafish ◽  
2021 ◽  
Author(s):  
Indra Wibowo ◽  
Nuruliawaty Utami ◽  
Tjandra Anggraeni ◽  
Anggraini Barlian ◽  
Ramadhani Eka Putra ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Caudal Fin ◽  
Fin Regeneration ◽  
Combined Administration

Exploring the molecular link between swim-training and caudal fin development in zebrafish (Danio rerio ) larvae

2014 ◽  
Vol 30 (4) ◽  
pp. 753-761 ◽  
Author(s):  
A. W. Fiaz ◽  
K. M. Léeon-Kloosterziel ◽  
J. L. van Leeuwen ◽  
S. Kranenbarg
Keyword(s):  
Danio Rerio ◽  
Caudal Fin ◽  
Fin Development

scholarly journals Tricaine Methanesulfonate (MS222) Has Short-Term Effects on Young Adult Zebrafish (Danio rerio) Working Memory and Cognitive Flexibility, but Not on Aging Fish

2021 ◽  
Vol 15 ◽  
Author(s):  
Barbara D. Fontana ◽  
Nancy Alnassar ◽  
Matthew O. Parker
Keyword(s):  
Working Memory ◽  
Cognitive Flexibility ◽  
Danio Rerio ◽  
Drug Exposure ◽  
Housing System ◽  
Biomedical Sciences ◽  
Behavioral Experiments ◽  
Anesthetic Drugs ◽  
Routine Procedures ◽  
Tricaine Methanesulfonate

Exposure to anesthetic drugs is common in biomedical sciences being part of routine procedures in different translational species, however its impacts on memory and cognition are still debated, having different impacts depending on drug and age. The zebrafish (Danio rerio) is a translational species widely used in behavioral neuroscience, where tricaine methanesulfonate (MS222) is the most acceptable and used drug when conducting routine procedures. Based on this, we investigated the effects of MS222 (100 mg/l) in young adults and aging zebrafish 1, 2, 3, and 7 days after exposure. Animals’ were submitted to the anesthetic procedure until loss of body posture, slowing of opercular movements and lack of response to tail touch with a plastic pipette were achieved, then further left in the drug for 3 min. After that, animals (6 mpf vs. 24 mpf) were transferred to a recovery tank until fully recovered and transferred back to their housing system until further testing in the free movement pattern (FMP) Y-maze, which assesses zebrafish working memory and cognitive lexibility. Young animals had significant impairment in their working memory and cognitive flexibility 1 and 2 days after the exposure to MS222, being fully recovered by day 3 and with no effects 7 days post drug exposure. Increased repetitions were also observed for animals exposed to MS222 which could indicate increased stress-related response in animals up to 2 days after drug exposure. No drug effect was observed in aging animals besides their natural decreased alternations and working memory. Overall, behavioral experiments after routine procedures using MS222 should be performed with caution and need to be delayed, at least 3 days after exposure where working memory, cognitive flexibility, and repetitive behavior are back to normal.

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