Tricaine methanesulfonate (MS-222) on the spermatic quality of zebrafish, Danio rerio

Aquaculture ◽  
2021 ◽  
Vol 533 ◽  
pp. 736090
Author(s):  
Marina Zanin ◽  
Antonio Sergio Varela Junior ◽  
Izani Bonel Acosta ◽  
Stela Mari Meneghello Gheller ◽  
Etiane Zimermann ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Tricaine Methanesulfonate

Assessment of sediment quality of Yangtze River estuary using zebrafish (Danio rerio) embryos

2009 ◽  
Vol 25 (3) ◽  
pp. 234-242 ◽  
Author(s):  
Lingling Wu ◽  
Ling Chen ◽  
Junli Hou ◽  
Yalei Zhang ◽  
Jianfu Zhao ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Yangtze River ◽  
Sediment Quality ◽  
River Estuary ◽  
Yangtze River Estuary ◽  
Danio Rerio Embryos

scholarly journals Integrated Assessment of the Quality of Sediments of Open Pit Lakes and the Impact on the Environment

2019 ◽  
Author(s):  
Viniciu Fagundes Barbara ◽  
Daniela de Melo e Silva ◽  
Rosana Gonçalves Barros ◽  
Aldo Muro Jr ◽  
Nelson Roberto Antoniosi Filho
Keyword(s):  
Danio Rerio ◽  
Mining Industry ◽  
Open Pit ◽  
Control Group ◽  
Dna Damages ◽  
Gold Mines ◽  
Pit Lakes ◽  
Test Organisms ◽  
The Impact

The mining industry is known for the intense environmental impacts it triggers, especially when it is developed in an open environment. Pit lakes are formed in depleted deposits and may be promising opportunities for use by society as well as troubling environmental liabilities. While these artificial basins are increasing numerically in many parts of the world, they are still little known researchers in the Environmental Sciences, which makes their environmental management challenging. The main objective of this study was to evaluate the environmental quality of sediments from three deactivated open-pit gold mines, located in the Mara Rosa, Brazil, through chemical, ecotoxicological and genotoxicology analyses. For this purpose, we collected samples in the dry season boom, and subsequently, we analysed metals. In sequence, acute ecotoxicological and a genotoxicology test (comet assay) were developed with Danio rerio fishes, in concentrations of 3.12%; 6.25%; 12.5%; 25%; 50% and 100%, in addition to the control group. The results indicated that the three lakes are environmentally compromised, especially Lago Azul, whose waters and sediments are undergoing an intense process of geological conditioning. Our results did not verify the ecotoxicity of the sediments of any of the lakes, only behavioural alterations in the test organisms exposed to the concentrations of 25%, 50% and 100% of the samples obtained in the Lago Azul. About the sediments, DNA damage at Danio rerio was detected in the three investigated environments, although fishes kept in the water sampled at Lago Azul presented the most extension of DNA damages.

scholarly journals Tricaine Methanesulfonate (MS222) Has Short-Term Effects on Young Adult Zebrafish (Danio rerio) Working Memory and Cognitive Flexibility, but Not on Aging Fish

2021 ◽  
Vol 15 ◽  
Author(s):  
Barbara D. Fontana ◽  
Nancy Alnassar ◽  
Matthew O. Parker
Keyword(s):  
Working Memory ◽  
Cognitive Flexibility ◽  
Danio Rerio ◽  
Drug Exposure ◽  
Housing System ◽  
Biomedical Sciences ◽  
Behavioral Experiments ◽  
Anesthetic Drugs ◽  
Routine Procedures ◽  
Tricaine Methanesulfonate

Exposure to anesthetic drugs is common in biomedical sciences being part of routine procedures in different translational species, however its impacts on memory and cognition are still debated, having different impacts depending on drug and age. The zebrafish (Danio rerio) is a translational species widely used in behavioral neuroscience, where tricaine methanesulfonate (MS222) is the most acceptable and used drug when conducting routine procedures. Based on this, we investigated the effects of MS222 (100 mg/l) in young adults and aging zebrafish 1, 2, 3, and 7 days after exposure. Animals’ were submitted to the anesthetic procedure until loss of body posture, slowing of opercular movements and lack of response to tail touch with a plastic pipette were achieved, then further left in the drug for 3 min. After that, animals (6 mpf vs. 24 mpf) were transferred to a recovery tank until fully recovered and transferred back to their housing system until further testing in the free movement pattern (FMP) Y-maze, which assesses zebrafish working memory and cognitive lexibility. Young animals had significant impairment in their working memory and cognitive flexibility 1 and 2 days after the exposure to MS222, being fully recovered by day 3 and with no effects 7 days post drug exposure. Increased repetitions were also observed for animals exposed to MS222 which could indicate increased stress-related response in animals up to 2 days after drug exposure. No drug effect was observed in aging animals besides their natural decreased alternations and working memory. Overall, behavioral experiments after routine procedures using MS222 should be performed with caution and need to be delayed, at least 3 days after exposure where working memory, cognitive flexibility, and repetitive behavior are back to normal.

Effect of glyphosate on the sperm quality of zebrafish Danio rerio

2014 ◽  
Vol 155 ◽  
pp. 322-326 ◽  
Author(s):  
Fernanda Moreira Lopes ◽  
Antonio Sergio Varela Junior ◽  
Carine Dahl Corcini ◽  
Alessandra Cardoso da Silva ◽  
Vitória Gasperin Guazzelli ◽  
...  
Keyword(s):  
Danio Rerio ◽  
Sperm Quality

scholarly journals A Comparison of Buffered Tricaine Methanesulfonate (MS-222) and Isoeugenol Anesthesia for Caudal Fin Clipping in Zebrafish (Danio rerio)

2020 ◽  
Vol 59 (6) ◽  
pp. 732-736
Author(s):  
Gabrielle C Musk ◽  
Ben J Ezzy ◽  
Lisa M Kenchington ◽  
Wendy A Hopper ◽  
Lauren M Callahan
Keyword(s):  
Danio Rerio ◽  
Caudal Fin ◽  
Safety And Efficacy ◽  
Monitoring Time ◽  
Recovery From Anesthesia ◽  
Tricaine Methanesulfonate ◽  
Time Point

The purpose of this study was to compare the safety and efficacy of buffered tricaine methanesulfonate (MS-222) and isoeugenol for the anesthesia of zebrafish undergoing caudal fin clipping. Eighty 9 mo Danio rerio (AB strain) zebrafish were allocated to one of 2 equal groups: buffered MS-222 (168 mg/L, n = 40) or isoeugenol (20 mg/L, n = 40). The time to induction of anesthesia was significantly shorter in the isoeugenol group (141 ± 70 s) than in the MS-222 group (207 ± 103 s). The time to recovery from anesthesia was also shorter in the MS-222 group (373 ± 125 s) than in the isoeugenol group (491 ± 176 s). No obvious displays of distress or aversion to anesthesia were observed in either group. No difference was detected in the proportion of zebrafish that became anesthetized with either drug. One male zebrafish in the buffered MS-222 group was found dead at the 1-h post-procedural monitoring time point, but there was no difference between groups in the proportion of fish that survived anesthesia to the end of experiment. In conclusion, the safety and efficacy of buffered MS-222 (168 mg/L) and isoeugenol (20 mg/L) was similar for zebrafish undergoing anesthesia for caudal fin clipping.

scholarly journals Fishing for Nature’s Hits: Establishment of the Zebrafish as a Model for Screening Antidiabetic Natural Products

2015 ◽  
Vol 2015 ◽  
pp. 1-16 ◽  
Author(s):  
Nadia Tabassum ◽  
Hongmei Tai ◽  
Da-Woon Jung ◽  
Darren R. Williams
Keyword(s):  
Natural Products ◽  
Natural Product ◽  
Danio Rerio ◽  
Morbidity Rate ◽  
Diabetes Research ◽  
Drug Candidates ◽  
Life Threatening ◽  
Screening Systems

Diabetes mellitus affects millions of people worldwide and significantly impacts their quality of life. Moreover, life threatening diseases, such as myocardial infarction, blindness, and renal disorders, increase the morbidity rate associated with diabetes. Various natural products from medicinal plants have shown potential as antidiabetes agents in cell-based screening systems. However, many of these potential “hits” fail in mammalian tests, due to issues such as poor pharmacokinetics and/or toxic side effects. To address this problem, the zebrafish (Danio rerio) model has been developed as a “bridge” to provide an experimentally convenient animal-based screening system to identify drug candidates that are activein vivo. In this review, we discuss the application of zebrafish to drug screening technologies for diabetes research. Specifically, the discovery of natural product-based antidiabetes compounds using zebrafish will be described. For example, it has recently been demonstrated that antidiabetic natural compounds can be identified in zebrafish using activity guided fractionation of crude plant extracts. Moreover, the development of fluorescent-tagged glucose bioprobes has allowed the screening of natural product-based modulators of glucose homeostasis in zebrafish. We hope that the discussion of these advances will illustrate the value and simplicity of establishing zebrafish-based assays for antidiabetic compounds in natural products-based laboratories.

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