scholarly journals DNAbased detection of western gall rust

2004 ◽  
Vol 57 ◽  
pp. 146-150 ◽  
Author(s):  
T.D. Ramsfield ◽  
D.R. Vogler
Keyword(s):  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Rust Fungus ◽  
Its Region ◽  
Pcr Primers ◽  
Host Tissue ◽  
Nuclear Ribosomal Dna ◽  
Infected Host ◽  
The Novel ◽  
Pcr Product

The internal transcribed spacer (ITS) region of the nuclear ribosomal DNA of the western gall rust fungus (Peridermium harknessii) was amplified using the basidiomycetespecific PCR primers ITS1F and ITS4B The PCR product was then sequenced and aligned with other pine stem rust ITS sequences and a conserved region within P harknessii was targeted with the novel PCR primer Phar1 Our PCR protocol was able to differentiate P harknessii from Cronartium comandrae and C coleosporioides and detected P harknessii within infected host tissue However P harknessii was not distinguishable from C quercuum fsp fusiforme The method provides a rapid and sensitive detection protocol for P harknessii and C quercuum fsp fusiforme within infected host tissue

Peucedanum huangshanense (Apiaceae), a new species from Anhui, China

Phytotaxa ◽  
2020 ◽  
Vol 430 (1) ◽  
pp. 17-24
Author(s):  
SHAN-SHAN CHU ◽  
DE-QUN WANG ◽  
HUA-SHENG PENG ◽  
LU-QI HUANG
Keyword(s):  
New Species ◽  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Its Region ◽  
Distinct Species ◽  
Nuclear Ribosomal Dna ◽  
Phylogenetic Position ◽  
A New Species

Peucedanum huangshanense, a new species discovered in Anhui, China, is illustrated and described. Detailed morphological comparisons have showed that it is similar to P. praeruptorum but differs from the latter by having larger compound umbels (5–14 cm across), rays up to 25, and long-ovoid mericarps with lateral ribs narrowly winged. To explore the phylogenetic position of this species, nuclear ribosomal DNA internal transcribed spacer (ITS) region was sequenced for P. huangshanense and P. praeruptorum collected in different places. The morphological and molecular evidences support the hypothesis that P. huangshanense is a new distinct species.

scholarly journals Teleomorph Formation of Phaeoacremonium aleophilum, Cause of Esca and Grapevine Decline in California

Plant Disease ◽  
2005 ◽  
Vol 89 (2) ◽  
pp. 177-184 ◽  
Author(s):  
S. Rooney-Latham ◽  
A. Eskalen ◽  
W. D. Gubler
Keyword(s):  
Vitis Vinifera ◽  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Type System ◽  
Its Region ◽  
Wood Chips ◽  
Nuclear Ribosomal Dna ◽  
Mating Types ◽  
Molecular Analyses

Phaeoacremonium is a recently described genus of the hyphomycetes and includes species associated with grapevine (Vitis vinifera) declines worldwide. Spores of Phaeoacremonium spp. have been trapped in infested vineyards, but neither asexual nor sexual fruiting structures have been observed in the field. Mating studies were carried out to determine if California P. aleophilum isolates are capable of forming a teleomorph in vitro. Sterilized grapevine shavings were placed on the surface of water agar plates with pairs of different California isolates of P. aleophilum, an isolate from the holotype of P. aleophilum, plus other related Phaeoacremonium spp. After approximately 28 to 35 days, perithecia were seen forming on wood chips and agar of many pairings. Upon maturation, fertile perithecia had gelatinous droplets of ascospores oozing from their ostioles. Successful crosses, resulting in mature perithecia, corresponded to a heterothallic mating type system. When F1 progeny were backcrossed with their parents, heterothallism was confirmed. Molecular analyses of the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA from ascospore progeny and perithecia confirmed that these perithecia were the teleomorph of P. aleophilum, Togninia minima. Furthermore, 4 months after moist incubating grapevine pieces from naturally infected vineyards, mature perithecia of T. minima could be seen forming on the xylem and pith tissues, suggesting both mating types occur on the same vine.

A molecular phylogeny of the grass family (Poaceae) based on the sequences of nuclear ribosomal DNA (ITS)

1998 ◽  
Vol 11 (6) ◽  
pp. 667 ◽  
Author(s):  
C. Hsiao ◽  
S. W. L. Jacobs ◽  
N. J. Chatterton ◽  
K. H. Asay
Keyword(s):  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
C4 Photosynthesis ◽  
Its Region ◽  
Nuclear Ribosomal Dna ◽  
Plate Tectonic ◽  
Its Phylogeny ◽  
The Core ◽  
Grass Family ◽  
Fossil Records

Phylogenetic relationships of the grass family inferred from the sequences of the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA (rDNA) are generally in accord with the boundaries of the six commonly recognised subfamilies: Bambusoideae, Pooideae, Arundinoideae, Centothecoideae, Chloridoideae and Panicoideae. ITS phylogeny recognises the herbaceous bamboo allies, Streptochaeta and Pharus, as the basal taxa to all grasses. The core grass lineage is resolved into three major clades: the basal Bambusoideae is sister to the monophyletic Pooideae and the Panicoideae–Arundinoideae–Centothecoideae–Chloridoideae (PACC) clade. Several genera with uncertain taxonomic affinities, Lygeum, Nardus, Brachyelytrum, Diarrhena, Anisopogon, Ampelodesmos, and the tribe Stipeae, are all clustered with a broadly defined Pooideae, and may be the ‘missing links’ between the Pooideae and the Bambusoideae. Relationships of the PACC clade indicate that C4 photosynthesis evolved independently among and within the PACC subfamilies. ITS phylogeny of the grass family, and evidence from the chloroplast genome, cytogenetics, fossil records, biogeography, and plate tectonic theory, suggest that the origin of the grasses is probably ‘out of South America’.

scholarly journals (269) Taxonomic Relationship of Korean Native Aster, Based on Internal Transcribed Spacer (ITS) Sequences of Nuclear Ribosomal DNA

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1017A-1017
Author(s):  
Su-Young Hong ◽  
Kwang-Soo Cho ◽  
Ki-Oug Yoo ◽  
Jong-Taek Suh ◽  
Dong-Lim Yoo
Keyword(s):  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Phylogenetic Trees ◽  
Its Region ◽  
Sister Group ◽  
Nuclear Ribosomal Dna ◽  
Phylogenetic Position ◽  
Its2 Region ◽  
Base Pairs ◽  
Total Length

There are 25 species of aster in Korea. There is a controversy about the taxonomical classification of Aster. The genus Aster was classified into four genera, Aster, Gymnaster, Kalimeris, and Heteropappus, by morphological characters. In order to clarify the phylogenetic position of aster, the nucleotide sequence of the nuclear ribosomal DNA internal transcribed spacer (ITS) region was compared among 11 taxa in Korean native aster. The size of ITS1 and ITS2 ranged from 283 to 286 bp and from 251 to 257 bp, respectively. The size of 5.8S region was 164 bp in 11 taxa. The total length of ITS1, 5.8S and ITS2, A. tripolium was shown to be the shortest length, 701 bp; and A. scaber was shown to be the longest length, 706 bp. The G+C content of ITS1 ranged from 47.9% to 51.2% and ITS2 ranged from 52.2% to 55.1%. The range of each taxon was narrow. The total length of the character matrix was 708 characters. Among them, total indel showed 9; in the ITS1 region indel showed 6 it was 67%; and in the ITS2 region, indel showed 3. Most of the indels showed deletion or insertion of only one base pair, but in A. spathulifolius deleted two base pairs and in A. tripolium deleted five base pairs. But in A. yomena, A. hayatae, A. koraiensis, and A. hispidus, the indel was not detected. Phylogenetic trees did not even make a difference inter-genus, but A. yomena and A. koraiensis called genus Kalimeris and genus Gymnaster, respectively; these constituted a clade. A. hispidus called genus Heteropappus was placed as a sister group to the clade of A. ageratoides and A. glehni.

Variation in the nuclear ribosomal DNA internal transcribed spacer (ITS) region of Pinus rzedowskii revealed by PCR-RFLP

1998 ◽  
Vol 96 (3-4) ◽  
pp. 539-544 ◽  
Author(s):  
A. Quijada ◽  
A. Liston ◽  
P. Delgado ◽  
A. Vázquez-Lobo ◽  
E. R. Alvarez-Buylla
Keyword(s):  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Its Region ◽  
Nuclear Ribosomal Dna ◽  
Pcr Rflp

Relationships among problematic North American stagnicoline snails (Pulmonata: Lymnaeidae) reinvestigated using nuclear ribosomal DNA internal transcribed spacer sequences

1997 ◽  
Vol 75 (9) ◽  
pp. 1540-1545 ◽  
Author(s):  
E. A. Remigio ◽  
D. Blair
Keyword(s):  
North America ◽  
Ribosomal Dna ◽  
Internal Transcribed Spacer ◽  
Its Region ◽  
Nucleotide Sequences ◽  
Its Sequences ◽  
Nuclear Ribosomal Dna ◽  
Snail Species ◽  
Separate Species ◽  
Internal Transcribed Spacer Sequences

The taxonomic relationships among four stagnicoline snail species from North America were reassessed by analysing nucleotide sequences from their nuclear ribosomal internal transcribed spacer (ITS) region. Three of the species (Stagnicola catascopium, Stagnicola emarginata, and Stagnicola elodes) are representatives of the subgenus Stagnicola s.str. from the northern U.S.A. and the fourth is a closely related Canadian species (Stagnicola caperata) belonging to the subgenus Hinkleyia. Members of the two subgenera differed considerably in their ITS sequences, demonstrating the usefulness of these sequences for distinguishing species from closely related lymnaeid subgenera. In contrast, ITS sequences for the three members of the subgenus Stagnicola s.str. were very similar, which supports an earlier view that they may not be separate species.

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