Longitudinal clearance of seasonal influenza A viral RNA measured by real-time polymerase chain reaction in patients identified at a hospital emergency department

2010 ◽  
Vol 42 (9) ◽  
pp. 679-686 ◽  
Author(s):  
Anna C. Nilsson ◽  
Maria Brytting ◽  
Filiz Serifler ◽  
Per Björkman ◽  
Kenneth Persson ◽  
...  
Keyword(s):  
Emergency Department ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Influenza A ◽  
Seasonal Influenza ◽  
Viral Rna ◽  
Hospital Emergency Department ◽  
Hospital Emergency ◽  
Chain Reaction ◽  
Polymerase Chain

Detection of avian influenza A/H7N9/2013 virus by real-time reverse transcription-polymerase chain reaction

2014 ◽  
Vol 206 ◽  
pp. 140-143 ◽  
Author(s):  
Xiaoping Kang ◽  
Weili Wu ◽  
Chuntao Zhang ◽  
Licheng Liu ◽  
Huahua Feng ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Avian Influenza ◽  
Real Time ◽  
Reverse Transcription ◽  
Influenza A ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Multiplex Real-Time Reverse-Transcription Polymerase Chain Reaction Assays for Diagnostic Testing of Severe Acute Respiratory Syndrome Coronavirus 2 and Seasonal Influenza Viruses: A Challenge of the Phase 3 Pandemic Setting

2020 ◽  
Author(s):  
Fabiola Mancini ◽  
Fabrizio Barbanti ◽  
Maria Scaturro ◽  
Stefano Fontana ◽  
Angela Di Martino ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Severe Acute Respiratory Syndrome ◽  
Real Time ◽  
Reverse Transcription ◽  
Seasonal Influenza ◽  
Influenza Viruses ◽  
Activity Period ◽  
Clinical Samples ◽  
Chain Reaction ◽  
Polymerase Chain

Abstract Background Pandemic coronavirus disease 2019 (COVID-19) disease represents a challenge for healthcare structures. The molecular confirmation of samples from infected individuals is crucial and therefore guides public health decision making. Clusters and possibly increased diffuse transmission could occur in the context of the next influenza season. For this reason, a diagnostic test able to discriminate severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from influenza viruses is urgently needed. Methods A multiplex real-time reverse-transcription polymerase chain reaction (PCR) assay was assessed using 1 laboratory protocol with different real-time PCR instruments. Overall, 1000 clinical samples (600 from samples SARS-CoV-2–infected patients, 200 samples from influenza-infected patients, and 200 negative samples) were analyzed. Results The assay developed was able to detect and discriminate each virus target and to intercept coinfections. The limit of quantification of each assay ranged between 5 and 10 genomic copy numbers, with a cutoff value of 37.7 and 37.8 for influenza and SARS-CoV-2 viruses, respectively. Only 2 influenza coinfections were detected in COVID-19 samples. Conclusions This study suggests that multiplex assay is a rapid, valid, and accurate method for the detection of SARS-CoV-2 and influenza viruses in clinical samples. The test may be an important diagnostic tool for both diagnostic and surveillance purposes during the seasonal influenza activity period.

scholarly journals Pandemic (H1N1) 2009 Cases in Nepal

2012 ◽  
Vol 52 (188) ◽  
Author(s):  
N Kandel ◽  
J M Shrestha ◽  
B Upadhyay ◽  
A K Shrestha ◽  
G Shakya
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Reverse Transcription ◽  
Pandemic Influenza ◽  
Influenza A ◽  
Pandemic H1n1 ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Keywords Influenza

We analyzed the data available in Nepal during this pandemic in order to determine the epidemiological, clinical and virological characteristics of pandemic influenza A in 2009. The test was conducted by real-time Reverse Transcription – Polymerase Chain Reaction on sample from patients with suspected influenza-like illnesses. Out of 538 cases were tested, 32 % were positive for pandemic influenza A 2009 and the infection rate was highest for cases of 11-20 years and lowest in >50 years of age. Keywords: Influenza A ; pandemic; RT-PCR; surveillance.

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