Identification and Characterization of Aneuploidy-Inducing Agents by DNA-Protein Flow Cytometry in V79 Chinese Hamster Cells

1993 ◽  
Vol 3 (1) ◽  
pp. 37-50 ◽  
Author(s):  
P. Maier ◽  
R. Kränzlin ◽  
R. Fasciati
Keyword(s):  
Flow Cytometry ◽  
Chinese Hamster ◽  
Chinese Hamster Cells ◽  
Identification And Characterization

Assessment of Persisting Chromosome Aberrations by Flow Karyotyping of Cloned Chinese Hamster Cells

1988 ◽  
Vol 43 (11-12) ◽  
pp. 948-954 ◽  
Author(s):  
Friedrich J. Otto
Keyword(s):  
Flow Cytometry ◽  
High Resolution ◽  
Cell Lines ◽  
Chromosomal Aberrations ◽  
Chromosome Aberrations ◽  
Chinese Hamster ◽  
Chinese Hamster Cells ◽  
Cell Clones ◽  
Permanent Cell

Abstract A preparation, staining and measuring protocol for high resolution flow cytometry of chromosomes was developed. This method allows us to identify all chromosome types and is suited for characterization of permanent cell lines and cell clones by establishing their flow karyotypes. In cell clones this procedure can be used for the detection of chromosomal aberrations which appear spontaneously or are induced by mutagen treatment and persist in the cell population.

Immunological and genetic characterization of 2-deoxygalactose-resistant, galactokinase-deficient mutants of Chinese hamster cells: evidence for structural mutations at the galK locus

1984 ◽  
Vol 4 (11) ◽  
pp. 2413-2419
Author(s):  
B Talbot ◽  
C A de Souza ◽  
D Banville ◽  
J P Thirion
Keyword(s):  
Structural Gene ◽  
Genetic Characterization ◽  
Chinese Hamster ◽  
Gene Complementation ◽  
Chinese Hamster Cells ◽  
Genetic Unit ◽  
Structural Mutations ◽  
Galactokinase Activity ◽  
Correct Expression

Ten independent mutants resistant to 2-deoxygalactose and without any detectable galactokinase activity (null-galactokinase mutations) were isolated from mutagenized Chinese hamster somatic cells. They were analyzed for the presence of serologically cross-reacting material (CRM) with antiserum generated against highly purified Chinese hamster galactokinase. All 10 mutants contain cross-reacting material (i.e., were CRM+), indicating that all the mutations affect the correct expression of a product of the galactokinase structural gene. Complementation analysis among them shows that the 10 mutations fall in one functional genetic unit.

Identification and characterization of liver nonparenchymal cells by flow cytometry

Hepatology ◽  
1987 ◽  
Vol 7 (4) ◽  
pp. 696-703 ◽  
Author(s):  
Mark Doolittle ◽  
Roger Bohman ◽  
Andres Durstenfeld ◽  
Joseph Cascarano
Keyword(s):  
Flow Cytometry ◽  
Nonparenchymal Cells ◽  
Identification And Characterization
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