Assessment of Persisting Chromosome Aberrations by Flow Karyotyping of Cloned Chinese Hamster Cells

1988 ◽  
Vol 43 (11-12) ◽  
pp. 948-954 ◽  
Author(s):  
Friedrich J. Otto
Keyword(s):  
Flow Cytometry ◽  
High Resolution ◽  
Cell Lines ◽  
Chromosomal Aberrations ◽  
Chromosome Aberrations ◽  
Chinese Hamster ◽  
Chinese Hamster Cells ◽  
Cell Clones ◽  
Permanent Cell

Abstract A preparation, staining and measuring protocol for high resolution flow cytometry of chromosomes was developed. This method allows us to identify all chromosome types and is suited for characterization of permanent cell lines and cell clones by establishing their flow karyotypes. In cell clones this procedure can be used for the detection of chromosomal aberrations which appear spontaneously or are induced by mutagen treatment and persist in the cell population.

Isolation and characterization of amphotericin B-resistant cell lines in Chinese hamster cells

Cell ◽  
1978 ◽  
Vol 14 (2) ◽  
pp. 415-421 ◽  
Author(s):  
Katsuhiko Hidaka ◽  
Hideya Endo ◽  
Shin-ichi Akiyama ◽  
Michihiko Kuwano
Keyword(s):  
Amphotericin B ◽  
Cell Lines ◽  
Chinese Hamster ◽  
Resistant Cell ◽  
Chinese Hamster Cells ◽  
Isolation And Characterization

scholarly journals Application of Imaging Flow Cytometry for the Characterization of Intracellular Attributes in Chinese Hamster Ovary Cell Lines at the Single‐Cell Level

2019 ◽  
Vol 14 (7) ◽  
pp. 1800675 ◽  
Author(s):  
Eva Pekle ◽  
Andrew Smith ◽  
Guglielmo Rosignoli ◽  
Christopher Sellick ◽  
C. M. Smales ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Single Cell ◽  
Cell Lines ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster ◽  
Ovary Cell ◽  
Single Cell Level ◽  
Cell Level ◽  
Imaging Flow Cytometry

scholarly journals Differential activation of the hprt gene on the inactive X chromosome in primary and transformed Chinese hamster cells.

1989 ◽  
Vol 9 (4) ◽  
pp. 1635-1641 ◽  
Author(s):  
S G Grant ◽  
R G Worton
Keyword(s):  
Cell Lines ◽  
X Chromosome ◽  
Gene Activation ◽  
Chinese Hamster ◽  
Fibroblast Cell ◽  
Dna Demethylation ◽  
Hprt Gene ◽  
Primary Fibroblast ◽  
Chinese Hamster Cells ◽  
Inactive X Chromosome

We have investigated the genetic activation of the hprt (hypoxanthine-guanine phosphoribosyltransferase) gene located on the inactive X chromosome in primary and transformed female diploid Chinese hamster cells after treatment with the DNA methylation inhibitor 5-azacytidine (5azaCR). Mutants deficient in HPRT were first selected by growth in 6-thioguanine from two primary fibroblast cell lines and from transformed lines derived from them. These HPRT- mutants were then treated with 5azaCR and plated in HAT (hypoxanthine-methotrexate-thymidine) medium to select for cells that had reexpressed the hprt gene on the inactive X chromosome. Contrary to previous results with primary human cells, 5azaCR was effective in activating the hprt gene in primary Chinese hamster fibroblasts at a low but reproducible frequency of 2 x 10(-6) to 7 x 10(-6). In comparison, the frequency in independently derived transformed lines varied from 1 x 10(-5) to 5 x 10(-3), consistently higher than in the nontransformed cells. This increase remained significant when the difference in growth rates between the primary and transformed lines was taken into account. Treatment with 5azaCR was also found to induce transformation in the primary cell lines but at a low frequency of 4 x 10(-7) to 8 x 10(-7), inconsistent with a two-step model of transformation followed by gene activation to explain the derepression of hprt in primary cells. Thus, these results indicate that upon transformation, the hprt gene on the inactive Chinese hamster X chromosome is rendered more susceptible to action by 5azaCR, consistent with a generalized DNA demethylation associated with the transformation event or with an increase in the instability of an underlying primary mechanism of X inactivation.

Human Squamous Cell Carcinoma: Establishment and Characterization of New Permanent Cell Lines

1981 ◽  
Vol 107 (11) ◽  
pp. 703-710 ◽  
Author(s):  
C. J. Krause ◽  
T. E. Carey ◽  
R. W. Ott ◽  
C. Hurbis ◽  
K. D. McClatchey ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Lines ◽  
Squamous Cell ◽  
Human Squamous Cell Carcinoma ◽  
Permanent Cell

Specific chromosomal aberrations correlated to transformation in Chinese hamster cells

1992 ◽  
Vol 62 (1) ◽  
pp. 81-87 ◽  
Author(s):  
Silvana Simi ◽  
Antonio Musio ◽  
Lucia Vatteroni ◽  
Antonio Piras ◽  
Giuseppe Rainaldi
Keyword(s):  
Chromosomal Aberrations ◽  
Chinese Hamster ◽  
Chinese Hamster Cells

Similar 150-kilobase DNA sequences are amplified in independently derived methotrexate-resistant Chinese hamster cells

1985 ◽  
Vol 5 (4) ◽  
pp. 619-627
Author(s):  
M Montoya-Zavala ◽  
J L Hamlin
Keyword(s):  
Dna Sequence ◽  
Cell Lines ◽  
Dihydrofolate Reductase ◽  
Dna Sequences ◽  
Consensus Sequence ◽  
Chinese Hamster ◽  
Ovary Cell ◽  
Chinese Hamster Cells ◽  
Reductase Gene ◽  
Reductase Domain

We have isolated overlapping recombinant cosmids that represent 150 kilobases of contiguous DNA sequence from the amplified dihydrofolate reductase domain of a methotrexate-resistant Chinese hamster ovary cell line (CHOC 400). This sequence includes the 25-kilobase dihydrofolate reductase gene and an origin of DNA synthesis. Eight cosmids that span this domain have been utilized as radioactive hybridization probes to analyze the similarities among the dihydrofolate reductase amplicons in four independently derived methotrexate-resistant Chinese hamster cell lines. We have observed no significant differences among the four cell lines within the 150-kilobase DNA sequence that we have examined, except for polymorphisms that result from the amplification of one or the other of two possible alleles of the dihydrofolate reductase domain. We also show that the restriction patterns of the amplicons in these four resistant cell lines are virtually identical to that of the corresponding, unamplified sequence in drug-susceptible parental cells. Furthermore, measurements of the relative copy numbers of fragments from widely separated regions of the amplicon suggest that all fragments in this 150-kilobase region may be amplified in unison. Our data show that in methotrexate-resistant Chinese hamster cells, the amplified unit is large relative to the dihydrofolate reductase gene itself. Furthermore, within the 150-kilobase amplified consensus sequence that we have examined, significant rearrangements do not seem to occur during the amplification process.

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