scholarly journals Resistance Gene Analogs (RGA) as a tool in fruit tree's breeding

2009 ◽  
Vol 15 (1-2) ◽  
Author(s):  
A. Gutermuth ◽  
A. Pedryc
Keyword(s):  
Molecular Markers ◽  
Marker Assisted Selection ◽  
Resistance Breeding ◽  
Resistance Gene Analogs ◽  
R Gene ◽  
Fruit Traits ◽  
Major Objective ◽  
Fruit Species ◽  
Marker Design ◽  
Breeding Application

Breeding for pest and disease resistance comes as a major objective behind the fruit traits. To increase the effectiveness of fruit resistance breeding application of the Marker Assisted Selection ( MAS) is advantageous. For generating molecular markers which enable the following of interesting traits basically two methods are available: targeted marker design based on conservative region of already known Resistance ( R) gene sequences or randomly generated markers. The creation  and the application  of  these homology  based  markers  are the object of this review in  the main  temperate zone  fruit species.

scholarly journals Selection of powdery mildew resistant and susceptible grapevine genotypes with molecular markers

2007 ◽  
pp. 100-104
Author(s):  
Stella Molnár ◽  
Zsuzsanna Galbács ◽  
Gábor Halász ◽  
Sarolta Hoffmann ◽  
Anikó Veres ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Powdery Mildew ◽  
Marker Assisted Selection ◽  
Powdery Mildew Resistance ◽  
Resistance Breeding ◽  
Breeding Programs ◽  
Pcr Rflp ◽  
Muscadinia Rotundifolia ◽  
Selection Of ◽  
Grape Breeding

Incorporation of competitive quality and resistance against the most important fungal diseases (powdery and downy mildew) in a cultivar is one of the most important aims of grapevine breeding. In the 20th century, the most advanced results in grapevine resistance breeding were achieved by French researchers. They used resistant cultivars in more than 30% of their growing areas. In these varieties, North American wild Vitisspecies were the resistance gene sources. The discovery of immunity-like resistance of Muscadinia rotundifolia opened new perspectives in resistance breeding. M. rotundifolia harbours a dominant powdery mildew gene, providing resistance in highquality cultivars after back-crosses with V. vinifera varieties. M. rotundifolia has been involved in the Hungarian grape breeding programs since 1996, thanks to a French-Hungarian variety exchange. In addition to traditional selection methods, application of MAS (Marker Assisted Selection) based on various types ofmolecular markers, can provide additional tools for these efforts. Run1 locus, responsible for powdery mildew resistance, was identified in Muscadinia rotundifolia. Molecular markers closely linked to this locus are very significant in screening progenies deriving from M. rotundifolia and V. vinifera crosses, making possible the discrimination between resistant and susceptible genotypes at DNA level. In our analyses BC5 progeny of {(M. rotundifola×V. vinifera) BC4}×Cardinal (V. vinifera) tested for powdery symptoms were analysed with PCR-RFLP (GLP1- 12P1P3) and microsatellite markers (VMC4f3.1, VMC8g9). Our results proved the applicability of the linked markers and reliability of marker assisted selection.

scholarly journals Plant resistance and strategies for breeding resistant varieties

2002 ◽  
Vol 38 (SI 1 - 6th Conf EFPP 2002) ◽  
pp. S9-S14 ◽  
Author(s):  
D.A.C. Pink ◽  
P. Hand
Keyword(s):  
Plant Transformation ◽  
Marker Assisted Selection ◽  
Specific Resistance ◽  
Durable Resistance ◽  
Resistance Breeding ◽  
R Gene ◽  
R Genes ◽  
Resistant Varieties ◽  
Plant Phenotype ◽  
The Impact

An explanation of the ‘boom-bust’ cycle of resistance breeding was provided by the gene-for-gene relationship between a pathogen and its host. Despite this understanding, most R genes continued to be deployed singly and resistance has been ephemeral. The reasons for breeding ‘single R gene’ varieties are discussed. Alternative strategies for the deployment of R genes and the use of quantitative race non-specific resistance have been advocated in order to obtain durable resistance. The feasibility of both of these approaches is discussed taking into account the impact of technologies such as plant transformation and marker-assisted selection. A change in focus from durability of the plant phenotype to that of the crop phenotype is advocated.

scholarly journals Marker Assisted Selection (MAS) for chickpea Fusarium oxysporum wilt resistant genotypes using PCR based molecular markers

2014 ◽  
Vol 41 (10) ◽  
pp. 6755-6762 ◽  
Author(s):  
Zakia Ahmad ◽  
Abdul Samad Mumtaz ◽  
Abdul Ghafoor ◽  
Amjad Ali ◽  
Mohammad Nisar
Keyword(s):  
Molecular Markers ◽  
Fusarium Oxysporum ◽  
Marker Assisted Selection ◽  
Resistant Genotypes

DEVELOPING MOLECULAR MARKERS FOR MARKER ASSISTED SELECTION OF FIRE BLIGHT RESISTANT APPLE SEEDLINGS

2007 ◽  
pp. 117-122 ◽  
Author(s):  
A. Peil ◽  
M.-V. Hanke ◽  
H. Flachowsky ◽  
K. Richter ◽  
T. Garcia ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Marker Assisted Selection ◽  
Fire Blight ◽  
Selection Of

Molecular Markers and Their Use in Marker-Assisted Selection in Rice

Crop Science ◽  
2008 ◽  
Vol 48 (4) ◽  
pp. 1266-1276 ◽  
Author(s):  
K. K. Jena ◽  
D. J. Mackill
Keyword(s):  
Molecular Markers ◽  
Marker Assisted Selection

Development of Molecular Markers Closely Linked to the Potato Leafroll Virus Resistance Gene, Rlr etb , for use in Marker-Assisted Selection

2016 ◽  
Vol 93 (3) ◽  
pp. 203-212 ◽  
Author(s):  
Joseph C. Kuhl ◽  
Richard G. Novy ◽  
Jonathan L. Whitworth ◽  
Margaret S. Dibble ◽  
Brian Schneider ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Resistance Gene ◽  
Virus Resistance ◽  
Marker Assisted Selection ◽  
Potato Leafroll Virus ◽  
Leafroll Virus ◽  
Virus Resistance Gene

scholarly journals Different molecular markers to identify the fertility of sugar beet monogerm germplasm resources

2021 ◽  
Author(s):  
Haoqi Shi ◽  
Zhi Pi ◽  
Zedong Wu
Keyword(s):  
Molecular Markers ◽  
Nucleotide Sequence ◽  
Sugar Beet ◽  
Marker Assisted Selection ◽  
Enzyme Digestion ◽  
Germplasm Resources ◽  
Single Nucleotide

Abstract Experiments were conducted to validate different molecular markers associated with sugar beet breeding using available sugar beet monogerm germplasm resources to explore their effectiveness in different sugar beet populations. We used multiple primer pairs to amplify sugar beet monogerm sterile and maintainer lines in order to verify their polymorphism. For the nucleus Rf1 locus genotype enzyme digestion was also required to verify. The results showed that three pairs of primers, TR1, s17 and 11E8M4S, produced polymorphism when amplifying sugar beet sterile and maintainer lines; primers o7 and AB-18, although polymorphic, did not correlate significantly with sugar beet fertility; primer cpSSR-2 did not produce significant band differences when amplifying sterile and maintainer lines, however, the number of single nucleotide sequence repeats of base A needed to be further verified as a basis for differentiating sugar beet fertility. The polymorphism of 15 pairs of primers related to sugar beet fertility identification was verified and TR1, s17 and 11E8M4S could be used to differentiate sugar beet sterile lines from the maintainer lines and used for subsequent Marker-Assisted Selection (MAS).

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