scholarly journals Selection of powdery mildew resistant and susceptible grapevine genotypes with molecular markers

2007 ◽  
pp. 100-104
Author(s):  
Stella Molnár ◽  
Zsuzsanna Galbács ◽  
Gábor Halász ◽  
Sarolta Hoffmann ◽  
Anikó Veres ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Powdery Mildew ◽  
Marker Assisted Selection ◽  
Powdery Mildew Resistance ◽  
Resistance Breeding ◽  
Breeding Programs ◽  
Pcr Rflp ◽  
Muscadinia Rotundifolia ◽  
Selection Of ◽  
Grape Breeding

Incorporation of competitive quality and resistance against the most important fungal diseases (powdery and downy mildew) in a cultivar is one of the most important aims of grapevine breeding. In the 20th century, the most advanced results in grapevine resistance breeding were achieved by French researchers. They used resistant cultivars in more than 30% of their growing areas. In these varieties, North American wild Vitisspecies were the resistance gene sources. The discovery of immunity-like resistance of Muscadinia rotundifolia opened new perspectives in resistance breeding. M. rotundifolia harbours a dominant powdery mildew gene, providing resistance in highquality cultivars after back-crosses with V. vinifera varieties. M. rotundifolia has been involved in the Hungarian grape breeding programs since 1996, thanks to a French-Hungarian variety exchange. In addition to traditional selection methods, application of MAS (Marker Assisted Selection) based on various types ofmolecular markers, can provide additional tools for these efforts. Run1 locus, responsible for powdery mildew resistance, was identified in Muscadinia rotundifolia. Molecular markers closely linked to this locus are very significant in screening progenies deriving from M. rotundifolia and V. vinifera crosses, making possible the discrimination between resistant and susceptible genotypes at DNA level. In our analyses BC5 progeny of {(M. rotundifola×V. vinifera) BC4}×Cardinal (V. vinifera) tested for powdery symptoms were analysed with PCR-RFLP (GLP1- 12P1P3) and microsatellite markers (VMC4f3.1, VMC8g9). Our results proved the applicability of the linked markers and reliability of marker assisted selection.

scholarly journals Molecular Identification of Pm4 Powdery Mildew Resistant Gene in Oat

2018 ◽  
Vol 46 (2) ◽  
pp. 350-355 ◽  
Author(s):  
Sylwia Magdalena OKOŃ ◽  
Tomasz OCIEPA ◽  
Aleksandra NUCIA
Keyword(s):  
Molecular Markers ◽  
Powdery Mildew ◽  
Correlation Coefficient ◽  
Molecular Identification ◽  
Marker Assisted Selection ◽  
Mapping Population ◽  
Breeding Programs ◽  
Resistant Gene ◽  
Modern Breeding ◽  
Selection Of

The selection of specific plants with desirable traits supported by molecular markers is one of the most important tools in modern breeding programs, which lead to reduce time and cost of selection. The aim of presented study was identification of dominant markers associated with Pm4 powdery mildew resistant gene in oat. To identify dominant silicoDArT markers for Pm4 gene, F2 mapping population ‘Av1860’ × ‘Fuchs’ were analyzed using DArTseq methodology. Among obtained 46 230 silicoDArT markers, 126 markers were high correlated with resistance to powdery mildew in oat conditioned by Pm4 gene. Among selected markers, 48 sequences have been chosen for potential conversion into specific STS markers. Finally, only 20 were suitable for primer design. As a result, 5 converted markers amplified expected products in resistant bulks, 3 of them segregated according to resistance in the whole population and shoved high correlation coefficient between marker and phenotype observation. Converted markers based on PCR could be used for identification of Pm4 gene in oat. Obtained results confirm the possibility of converting silicoDArT markers into PCR-based technique, which can be used in marker assisted selection (MAS).

scholarly journals Evaluation and Pre-selection of New Grapevine Genotypes Resistant to Downy and Powdery Mildew, Obtained by Cross-Breeding Programs in Spain

2021 ◽  
Vol 12 ◽  
Author(s):  
Leonor Ruiz-García ◽  
Pilar Gago ◽  
Celia Martínez-Mora ◽  
José Luis Santiago ◽  
Diego J. Fernádez-López ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Powdery Mildew ◽  
Simple Sequence Repeat ◽  
Selection Process ◽  
Sequence Repeat ◽  
Sources Of Resistance ◽  
Breeding Programs ◽  
Phenotypic Resistance ◽  
Simple Sequence ◽  
Selection Of

The need to develop an environmentally friendly, sustainable viticulture model has led to numerous grapevine improvement programmes aiming to increase resistance to downy and powdery mildew. The success of such programmes relies on the availability of protocols that can quantify the resistance/susceptibility of new genotypes, and on the existence of molecular markers of resistance loci that can aid in the selection process. The present work assesses the degree of phenotypic resistance/susceptibility to downy and powdery mildew of 28 new genotypes obtained from crosses between “Monastrell” and “Regent.” Three genotypes showed strong combined resistance, making them good candidates for future crosses with other sources of resistance to these diseases (pyramiding). In general, laboratory and glasshouse assessments of resistance at the phenotype level agreed with the resistance expected from the presence of resistance-associated alleles of simple sequence repeat (SSR) markers for the loci Rpv3 and Ren3 (inherited from “Regent”), confirming their usefulness as indicators of likely resistance to downy and powdery mildew, respectively, particularly so for downy mildew.

scholarly journals Evaluation and identification of resistance to powdery mildew in Indian wheat varieties under artificially created epiphytotic

2016 ◽  
Vol 8 (2) ◽  
pp. 565-569 ◽  
Author(s):  
Vikas Gupta ◽  
R. Selvakumar ◽  
Satish Kumar ◽  
C. N. Mishra ◽  
V. Tiwari ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Resistance Breeding ◽  
Wheat Breeding ◽  
Wheat Varieties ◽  
Breeding Programs ◽  
The North ◽  
Present Context ◽  
Basic Studies ◽  
North Western

Wheat production is globally weighed down by several biotic factors of which rusts and powdery mildew are the most important. Powdery mildew, caused by Blumeria graminis f. sp. tritici, is becoming a disease of major importance in the North Western Plains Zone and Northern Hills Zone of the country. In the present context ofclimate variability, diseases like powdery mildew can assume greater importance in wheat breeding programs. Importance of basic studies on powdery mildew is the need of hour. A set of 370 Indian bread wheat, durum, dicoccum and triticale varieties were screened using mixture of natural occurring pathotypes from four locations(viz., Karnal, Ludhiana, Dhaulakuan and Yamunanagar) under polyhouse conditions. Data were recorded on the severity of infection based on 0-9 scale. Out of 370, only 23 varieties (Amrut, DDK 1025, DWR 1006, DWR 195, GW 1139, HD 4672, HD 4530, HD 2278, HD 1981, DDK 1001, HI 8627, Jay, TL 2942, DT 46, K 8020, DDK 1029, K 9107, K 816, Lok 1, MACS 6145, DDK 1009, NP 111 and NP 200) had shown immune reaction (0) whereas 150, 83 and 114 varieties have shown resistance (1-3), moderately susceptible (4-6) and highly susceptible (>6) response respectively against powdery mildew. Data indicated that there is an urgent need to broaden the genetic base of wheat by identifying and introgressing new sources of powdery mildew resistance. With limited sources of PM resistance available, above identified genotypes can be further used and characterized for resistance breeding programs in India.

Evaluation of resistant genotypes and their characterization using molecular markers linked for powdery mildew resistance in cucumber (Cucumis sativus L.)

2022 ◽  
pp. 1-6
Author(s):  
Susheel Sharma ◽  
Aejaz Ahmad Dar ◽  
Sachin Gupta ◽  
Ravinder Singh
Keyword(s):  
Molecular Markers ◽  
Disease Resistance ◽  
Powdery Mildew ◽  
Marker Assisted Selection ◽  
Powdery Mildew Resistance ◽  
Breeding Strategies ◽  
Linkage Studies ◽  
Cucumis Sativus L ◽  
Moderately Resistant ◽  
Resistant Genotypes

Abstract Powdery mildew (PM) is one of the most severe fungal diseases of cucumber that limits its production worldwide. In this study, 140 genotypes of cucumber were screened for disease resistance under field and artificial conditions, and then validated with eight known SSR markers linked to PM resistance. Among these genotypes, genotype GS140 was found resistant (R), whereas GS148, GS16 and GS70 were moderately resistant, and GS169 was found to be tolerant. Of all the eight markers, only C31, C80, C162, SSR16472 and SSR16881 amplified the reported linked allele. The 127 bp allele of SSR16881 was found to be associated with the lowest disease severity of 37.65%. The associated markers could further be verified for their usability using linkage studies and the contrast genotypes in the present study could serve as a tool for selection in future marker-assisted selection breeding strategies for PM resistance.

Development of marker sets useful in the early selection of Ren4 powdery mildew resistance and seedlessness for table and raisin grape breeding

2011 ◽  
Vol 124 (1) ◽  
pp. 23-33 ◽  
Author(s):  
Siraprapa Mahanil ◽  
David Ramming ◽  
Molly Cadle-Davidson ◽  
Christopher Owens ◽  
Amanda Garris ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Powdery Mildew Resistance ◽  
Early Selection ◽  
Mildew Resistance ◽  
Selection Of ◽  
Grape Breeding

scholarly journals Introgression of the Powdery Mildew Resistance Genes Pm60 and Pm60b from Triticum urartu to Common Wheat Using Durum as a ‘Bridge’

Pathogens ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 25
Author(s):  
Qiang Zhang ◽  
Yinghui Li ◽  
Yiwen Li ◽  
Tzion Fahima ◽  
Qian-Hua Shen ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Powdery Mildew ◽  
Common Wheat ◽  
Resistance Genes ◽  
Powdery Mildew Resistance ◽  
Resistance Breeding ◽  
Introgression Lines ◽  
Synthetic Hexaploid Wheat ◽  
Triticum Urartu ◽  
Mildew Resistance

Powdery mildew, caused by the fungus Blumeria graminis f. sp. tritici (Bgt), has limited wheat yields in many major wheat-production areas across the world. Introducing resistance genes from wild relatives into cultivated wheat can enrich the genetic resources for disease resistance breeding. The powdery mildew resistance gene Pm60 was first identified in diploid wild wheat Triticum urartu (T. urartu). In this study, we used durum as a ‘bridge’ approach to transfer Pm60 and Pm60b into hexaploid common wheat. Synthetic hexaploid wheat (SHW, AABBAuAu), developed by crossing T. urartu (AuAu) with durum (AABB), was used for crossing and backcrossing with common wheat. The Pm60 alleles were tracked by molecular markers and the resistance to powdery mildew. From BC1F1 backcross populations, eight recombinant types were identified based on five Pm60-flanking markers, which indicated different sizes of the introgressed chromosome segments from T. urartu. Moreover, we have selected two resistance-harboring introgression lines with high self-fertility, which could be easily used in wheat breeding system. Our results showed that the durum was an excellent ‘bridge’ for introducing the target gene from diploid T. urartu into the hexaploid cultivated wheat. Moreover, these introgression lines could be deployed in wheat resistance breeding programs, together with the assistance of the molecular markers for Pm60 alleles.

scholarly journals Mapping QTL for spike fertility and related traits in two doubled haploid wheat (Triticum aestivum L.) populations

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Nicole Pretini ◽  
Leonardo S. Vanzetti ◽  
Ignacio I. Terrile ◽  
Guillermo Donaire ◽  
Fernanda G. González
Keyword(s):  
Doubled Haploid ◽  
Marker Assisted Selection ◽  
Triticum Aestivum L ◽  
Physiological Model ◽  
Yield Potential ◽  
Breeding Programs ◽  
Per Se ◽  
Genomic Regions ◽  
Fertility Traits ◽  
Selection Of

Abstract Background In breeding programs, the selection of cultivars with the highest yield potential consisted in the selection of the yield per se, which resulted in cultivars with higher grains per spike (GN) and occasionally increased grain weight (GW) (main numerical components of the yield). In this study, quantitative trait loci (QTL) for GW, GN and spike fertility traits related to GN determination were mapped using two doubled haploid (DH) populations (Baguette Premium 11 × BioINTA 2002 and Baguette 19 × BioINTA 2002). Results In total 305 QTL were identified for 14 traits, out of which 12 QTL were identified in more than three environments and explained more than 10% of the phenotypic variation in at least one environment. Eight hotspot regions were detected on chromosomes 1A, 2B, 3A, 5A, 5B, 7A and 7B in which at least two major and stable QTL sheared confidence intervals. QTL on two of these regions (R5A.1 and R5A.2) have previously been described, but the other six regions are novel. Conclusions Based on the pleiotropic analysis within a robust physiological model we conclude that two hotspot genomic regions (R5A.1 and R5A.2) together with the QGW.perg-6B are of high relevance to be used in marker assisted selection in order to improve the spike yield potential. All the QTL identified for the spike related traits are the first step to search for their candidate genes, which will allow their better manipulation in the future.

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