The monodispersion situation of Multi-walled carbon nanotube dispersion is vital for fabricating high quality MWCNT buckypapers with vacuum filtration method. In this paper, the MWCNT buckypapers were fabricated by surfactants Triton-X100, sonication, centrifugation, vacuum filtration, rinsing and annealing processes. Transmission electron microscopy (TEM) and Zeta potential results show the maximum achievable separation has been reached. The properties of MWCNTs buckypaper can be characterized by Scanning electron microscopy (SEM), a four-point probe, N2 adsorption isotherm, TGA-DSC methods. The results showed that the buckypaper exhibits a low surface and volume density, a high porosity and electric conductivity. The pore diameter is up to 22.02nm, no substantial mass loss below 600°C in air.