scholarly journals Survey on Perkinsus Species in Manila Clam Ruditapes philippinarum in Korean Waters Using Species-Specific PCR

2017 ◽  
Vol 52 (4) ◽  
pp. 202-205 ◽  
Author(s):  
Hyun-Sil Kang ◽  
Hyun-Sung Yang ◽  
Kimberly S. Reece ◽  
Young-Ghan Cho ◽  
Hye-Mi Lee ◽  
...  
Keyword(s):  
Ruditapes Philippinarum ◽  
Manila Clam ◽  
Korean Waters ◽  
Specific Pcr ◽  
Species Specific

Are Juvenile Manila Clam Ruditapes philippinarum Free from Perkinsus olseni Infection in Korean Waters?

2020 ◽  
Vol 55 (4) ◽  
pp. 573-579
Author(s):  
Hye-Mi Lee ◽  
Young-Ghan Cho ◽  
Hee-Do Jeung ◽  
Min-Seok Jang ◽  
Jee Youn Hwang ◽  
...  
Keyword(s):  
Ruditapes Philippinarum ◽  
Manila Clam ◽  
Korean Waters

A species-specific PCR forLactobacillus inersdemonstrates a relative specificity of this species for vaginal colonization

2008 ◽  
Vol 20 (3) ◽  
Author(s):  
Mohammed A. Alqumber ◽  
Jeremy P. Burton ◽  
Celia Devenish ◽  
John R. Tagg
Keyword(s):  
Specific Pcr ◽  
Vaginal Colonization ◽  
Relative Specificity ◽  
Species Specific

Three way crosses between two-band red and white zebra strains of Manila clam, Ruditapes philippinarum

2011 ◽  
Vol 34 (8) ◽  
pp. 1190-1197
Author(s):  
Xi-wu YAN ◽  
Yue-huan ZHANG ◽  
Huan-qiang SUN ◽  
Zhong-ming HUO ◽  
Xin SUN ◽  
...  
Keyword(s):  
Ruditapes Philippinarum ◽  
Manila Clam

Preliminary study of establishment of families and their early growth and development for manila clam (Ruditapes philippinarum)

2010 ◽  
Vol 34 (1) ◽  
pp. 32-40
Author(s):  
Xi-wu YAN ◽  
Zhong-ming HUO ◽  
Yue-huan ZHANG ◽  
Feng YANG ◽  
Guo-fan ZHANG
Keyword(s):  
Growth And Development ◽  
Early Growth ◽  
Ruditapes Philippinarum ◽  
Manila Clam ◽  
Preliminary Study

scholarly journals The new PFAS C6O4 and its effects on marine invertebrates: First evidence of transcriptional and microbiota changes in the Manila clam Ruditapes philippinarum

2021 ◽  
Vol 152 ◽  
pp. 106484
Author(s):  
Ilaria Bernardini ◽  
Valerio Matozzo ◽  
Sara Valsecchi ◽  
Luca Peruzza ◽  
Giulia Dalla Rovere ◽  
...  
Keyword(s):  
Marine Invertebrates ◽  
Ruditapes Philippinarum ◽  
Manila Clam

Testing the enemy release hypothesis: trematode parasites in the non-indigenous Manila clam Ruditapes philippinarum

Hydrobiologia ◽  
2009 ◽  
Vol 630 (1) ◽  
pp. 139-148 ◽  
Author(s):  
C. Dang ◽  
X. de Montaudouin ◽  
J. Bald ◽  
F. Jude ◽  
N. Raymond ◽  
...  
Keyword(s):  
Ruditapes Philippinarum ◽  
Enemy Release Hypothesis ◽  
Manila Clam ◽  
Enemy Release ◽  
Trematode Parasites

scholarly journals Development and Validation of a Nested-PCR-Denaturing Gradient Gel Electrophoresis Method for Taxonomic Characterization of Bifidobacterial Communities

2003 ◽  
Vol 69 (11) ◽  
pp. 6380-6385 ◽  
Author(s):  
R. Temmerman ◽  
L. Masco ◽  
T. Vanhoutte ◽  
G. Huys ◽  
J. Swings
Keyword(s):  
Nested Pcr ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Temporal Changes ◽  
Rrna Gene ◽  
Specific Pcr ◽  
Gradient Gel Electrophoresis ◽  
Species Specific ◽  
Taxonomic Characterization

ABSTRACT The taxonomic characterization of a bacterial community is difficult to combine with the monitoring of its temporal changes. None of the currently available identification techniques are able to visualize a “complete” community, whereas techniques designed for analyzing bacterial ecosystems generally display limited or labor-intensive identification potential. This paper describes the optimization and validation of a nested-PCR-denaturing gradient gel electrophoresis (DGGE) approach for the species-specific analysis of bifidobacterial communities from any ecosystem. The method comprises a Bifidobacterium-specific PCR step, followed by purification of the amplicons that serve as template DNA in a second PCR step that amplifies the V3 and V6-V8 regions of the 16S rRNA gene. A mix of both amplicons is analyzed on a DGGE gel, after which the band positions are compared with a previously constructed database of reference strains. The method was validated through the analysis of four artificial mixtures, mimicking the possible bifidobacterial microbiota of the human and chicken intestine, a rumen, and the environment, and of two fecal samples. Except for the species Bifidobacterium coryneforme and B. indicum, all currently known bifidobacteria originating from various ecosystems can be identified in a highly reproducible manner. Because no further cloning and sequencing of the DGGE bands is necessary, this nested-PCR-DGGE technique can be completed within a 24-h span, allowing the species-specific monitoring of temporal changes in the bifidobacterial community.

Reproductive cycle of the Manila clam (Ruditapes philippinarum): an intensively harvested invasive species in the Tagus Estuary (Portugal)

2017 ◽  
Vol 98 (7) ◽  
pp. 1645-1657 ◽  
Author(s):  
Paula Moura ◽  
Paulo Vasconcelos ◽  
Fábio Pereira ◽  
Paula Chainho ◽  
José Lino Costa ◽  
...  
Keyword(s):  
Invasive Species ◽  
Reproductive Cycle ◽  
Sexual Maturity ◽  
Ruditapes Philippinarum ◽  
Manila Clam ◽  
Tagus Estuary ◽  
Gonad Maturation ◽  
Oocyte Diameter ◽  
Ruditapes Decussatus ◽  
Spawning Period

The present study aimed to describe the reproductive cycle and estimate the size at sexual maturity of the Manila clam (Ruditapes philippinarum) in the Tagus Estuary (Portugal). Specimens were collected monthly from September 2013 to December 2015 during fishing surveys using bivalve dredges. The gametogenic cycle was described in detail using gonad histology and monitored throughout the study period by the monthly variation in the frequency of gonad maturation stages, mean gonadal index and mean oocyte diameter. This invasive population of R. philippinarum displayed synchronous gonadal development between sexes, with ripening occurring mainly in April and May followed by an extensive spawning period until November–December. Individuals reached the size at first sexual maturity at 29.4 mm in shell length (i.e. before 1 year old). Furthermore, the reproductive strategy, dynamics and potential were compared between the invasive R. philippinarum and the native congeneric carpet shell clam (Ruditapes decussatus). Overall, the data gathered in this study constitutes valuable baseline information to propose conservation strategies and implement management measures to minimize the harmful impacts caused by this invasive species on local ecosystems and native biodiversity, particularly over populations of autochthonous bivalves.

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