scholarly journals Results of tick testing for detection of viruses of Karshi, Tamdy, Issyk-Kul fever, Syr Darya valley fever

Author(s):  
Talgat Nurmakhanov ◽  
◽  
Toktasyn Erubaev ◽  
Yerlan Sansyzbaev ◽  
Nurkeldy Turebekov ◽  
...  

In Kazakhstan natural foci of Crimea-Congo hemorrhagic fever is located on the territory of Turkestan, Kyzylorda and Zhambyl regions. Whereas preventive measures are taken, this disease is diagnosed annually among people, but there is a group of viruses such as Karshi, Tamdy, the Issyk-Kul fever virus and Syr Darya valley fever which are less known. In this regard the goal was set to identify the prevalence of viruses of Karshi, Tamdy, Issyk-Kul fever and fever of the Syr Darya valley in hemorrhagic fever endemic in the Crimea-Congo hemorrhagic fever to determine the main hosts and vectors of infection. Ticks captured in areas natural foci of the Crimean-Congo hemorrhagic fever virus. The species composition of captured ticks was represented by 9 species: Hyalomma scupense, Hyalomma asiaticum, Hyalomma turanicum, Hyalomma anatolicum, Haemaphysalis sucata, Haemaphysalis punctata, Dermacentor niveus, Rhipicephalus pumilio, Rhipicephalus schulzei. Preliminary work was carried out on the selection and design of oligonucleotide primers for the identification of viruses by molecular genetic analysis. As a result of the studies, positive samples were found for viruses of Tamdy and Syr Darya valley fever in ticks H. asiaticum, H. scupense from the Turkestan region. The Crimean-Congo hemorrhagic fever virus was detected in H. asiaticum and H. scupense ticks from Zhambyl and Turkestan regions.

2019 ◽  
Vol 65 (3) ◽  
pp. 451-461 ◽  
Author(s):  
Anne Rackow ◽  
Christa Ehmen ◽  
Ronald von Possel ◽  
Raquel Medialdea-Carrera ◽  
David Brown ◽  
...  

Abstract BACKGROUND The cellular surface molecule HsTOSO/FAIM3/HsFcμR has been identified as an IgM-specific Fc receptor expressed on lymphocytes. Here, we show that its extracellular immunoglobulin-like domain (HsFcμR-Igl) specifically binds to IgM/antigen immune complexes (ICs) and exploit this property for the development of novel detection systems for IgM antibodies directed against Crimean-Congo hemorrhagic fever virus (CCHFV) and Zika virus (ZIKV). METHODS His-tagged HsFcμR-Igl was expressed in Escherichia coli and purified by affinity chromatography, oxidative refolding, and size-exclusion chromatography. Specific binding of HsFcμR-Igl to IgM/antigen ICs was confirmed, and 2 prototypic ELISAs for the detection of anti-CCHFV and anti-ZIKV IgM antibodies were developed. Thereby, patient sera and virus-specific recombinant antigens directly labeled with horseradish peroxidase (HRP) were coincubated on HsFcμR-Igl-coated ELISA plates. Bound ICs were quantified by measuring turnover of a chromogenic HRP substrate. RESULTS Assay validation was performed using paired serum samples from 15 Kosovar patients with a PCR-confirmed CCHFV infection and 28 Brazilian patients with a PCR-confirmed ZIKV infection, along with a panel of a priori CCHFV/ZIKV-IgM-negative serum samples. Both ELISAs were highly reproducible. Sensitivity and specificity were comparable with or even exceeded in-house gold standard testing and commercial kits. Furthermore, latex beads coated with HsFcμR-Igl aggregated upon coincubation with an IgM-positive serum and HRP-labeled antigen but not with either component alone, revealing a potential for use of HsFcμR-Igl as a capture molecule in aggregation-based rapid tests. CONCLUSIONS Recombinant HsFcμR-Igl is a versatile capture molecule for IgM/antigen ICs of human and animal origin and can be applied for the development of both plate- and bead-based serological tests.


2021 ◽  
Vol 290 ◽  
pp. 114075
Author(s):  
Stephen Balinandi ◽  
Claudia von Brömssen ◽  
Alex Tumusiime ◽  
Jackson Kyondo ◽  
Hyesoo Kwon ◽  
...  

2020 ◽  
Vol 26 (2) ◽  
pp. 385-387
Author(s):  
Leholonolo Mathengtheng ◽  
Dominique Goedhals ◽  
Phillip A. Bester ◽  
Jacqueline Goedhals ◽  
Felicity J. Burt

Sign in / Sign up

Export Citation Format

Share Document