Orphan designation: Adeno-associated viral vector serotype 5 containing a B-domain deleted variant of human coagulation factor VIII gene, Treatment of haemophilia A

2018 ◽  
Author(s):  
Keyword(s):  
Factor Viii ◽  
Viral Vector ◽  
Coagulation Factor ◽  
Coagulation Factor Viii ◽  
Haemophilia A ◽  
Factor Viii Gene ◽  
Orphan Designation ◽  
Gene Treatment ◽  
Serotype 5

scholarly journals Bioengineered coagulation factor VIII enables long-term correction of murine hemophilia A following liver-directed adeno-associated viral vector delivery

2014 ◽  
Vol 1 ◽  
pp. 14036 ◽  
Author(s):  
Harrison C Brown ◽  
J Fraser Wright ◽  
Shangzhen Zhou ◽  
Allison M Lytle ◽  
Jordan E Shields ◽  
...  
Keyword(s):  
Factor Viii ◽  
Hemophilia A ◽  
Viral Vector ◽  
Coagulation Factor ◽  
Coagulation Factor Viii ◽  
Vector Delivery

Investigation of Underlying Reasons of Factor VIII Deficiency in Haemophilia A Patients with Undetectable Mutations in the Factor VIII Gene.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 1042-1042
Author(s):  
Osman El-Maarri ◽  
Jörg Schröder ◽  
Claudia Klein ◽  
Rainer Schwaab ◽  
Anne Goodeve ◽  
...  
Keyword(s):  
Factor Viii ◽  
Coagulation Factor ◽  
Causative Mutation ◽  
Haemophilia A ◽  
Rt Pcr ◽  
Factor Viii Gene ◽  
Total Blood ◽  
Reverse Transcription Pcr ◽  
Multi Center Study ◽  
Center Study

Abstract Haemophilia A (HA) is an X-linked bleeding disorder caused by heterogeneous mutations in the coagulation factor VIII (F8) gene. Despite applying sensitive methods for mutation detection, and after excluding the inversions mutations a causative mutation is not identified in F8 gene in about 2,5% of severe HA patients (53 patients out of 2350 German patients). Analysis of mRNA from a small group of such (German) patients has excluded mutations deep in the introns that may affect normal splicing or mechanisms causing some unknown rearrangements of the F8 gene as the cause of HA. Among this group, in one patient no F8 mRNA was detected. Using two common polymorphisms in F8 exon 14, we were able to show that the same allele shared by the patient, his mother and his sister was not detected by reverse transcription PCR (RT-PCR) from total blood mRNA. These findings strongly suggest that the cause of HA in this patient is either absence or rapid degradation of the F8 mRNA which points to a novel mechanism leading to HA. To further investigate the cause of Hemophilia A in these patients an international multi-center study was established. The aim of this study to assemble a large collection of such families that could provide clues for novel factors/mechanisims that are important for the F8 molecule biogenesis and protein regulation as well as F8 expression. This multi-center study is therefore suposed to identify novel mechanisms causing HA.

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