scholarly journals MOLECULAR-GENETIC CHARACTERIZATION OF PARAINFLUENZA-3 VIRUS ISOLATES

Author(s):  
A.K. Galiullin ◽  
◽  
A. Gueriche ◽  
V.G. Gumerov ◽  
A.Y. Shaeva ◽  
...  

This study reports on the molecular-genetic characterization of a field strain of BPIV-3 isolated in the Republic of Tatarstan. M gene fragments of the Russian isolate LD-9 and the vaccine strain PTK/86 were sequenced and aligned with the corresponding sequences of the virus strains presented in GenBank. The results of the phylogenetic analysis showed that the Russian isolate LD-9 (MW52481) and the vaccine strain PTK45/86 belong to the genotype A of the parainfluenza virus-3, as well as the reference strain SF-4. The Russian isolate LD-9 showed 98.3 % similarity with the reference strain SF-4, and the Russian vaccine strain PTK-45/86 was 100 % identical to the reference strain.

2008 ◽  
Vol 42 (1) ◽  
pp. 70-78 ◽  
Author(s):  
O. I. Kiselev ◽  
V. M. Blinov ◽  
M. M. Pisareva ◽  
V. A. Ternovoy ◽  
A. P. Agafonov ◽  
...  

2002 ◽  
Vol 184 (22) ◽  
pp. 6130-6137 ◽  
Author(s):  
Shara Allen ◽  
Julie L. Zilles ◽  
Diana M. Downs

ABSTRACT Together, the biosyntheses of histidine, purines, and thiamine pyrophosphate (TPP) contain examples of convergent, divergent, and regulatory pathway integration. Mutations in two purine biosynthetic genes (purI and purH) affect TPP biosynthesis due to flux through the purine and histidine pathways. The molecular genetic characterization of purI mutants and their respective pseudorevertants resulted in the conclusion that <1% of the wild-type activity of the PurI enzyme was sufficient for thiamine but not for purine synthesis. The respective pseudorevertants were found to be informational suppressors. In addition, it was shown that accumulation of the purine intermediate aminoimidazole carboxamide ribotide inhibits thiamine synthesis, specifically affecting the conversion of aminoimidazole ribotide to hydroxymethyl pyrimidine.


2016 ◽  
Vol 161 (5) ◽  
pp. 1261-1271 ◽  
Author(s):  
Surachet Benjathummarak ◽  
Chanon Fa-ngoen ◽  
Chonlatip Pipattanaboon ◽  
Khwanchit Boonha ◽  
Pongrama Ramasoota ◽  
...  

2006 ◽  
Vol 114 (1) ◽  
pp. 21-30 ◽  
Author(s):  
E. S. Lagudah ◽  
H. McFadden ◽  
R. P. Singh ◽  
J. Huerta-Espino ◽  
H. S. Bariana ◽  
...  

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