Adrenal Cortical Hormone and Incorporation of Radioactivity into Nucleic Acid of Isolated Rat Diaphragm.

Insulin in vitro stimulated the incorporation into the nucleic acid fraction of isolated rat diaphragm of radioactivity from d-glucose-U-C14, adenine-8-C14 and orotic acid-6-C14; insulin had no effect on the incorporation of thymine-2-C14 into muscle nucleic acids. Insulin enhanced the incorporation into nucleic acids of C14 from adenine and orotic acid in the absence of added glucose, and incorporation of adenine-8-C14 was not influenced by glucose concentration over the range 0–600 mg %.

Download Full-text

Effect of insulin on nucleic acid synthesis in isolated rat diaphragm

Biochimica et Biophysica Acta (BBA) - Specialized Section on Nucleic Acids and Related Subjects ◽

10.1016/0926-6550(63)90403-1 ◽

1963 ◽

Vol 68 ◽

pp. 28-33 ◽

Cited By ~ 40

Author(s):

Ira G. Wool

Keyword(s):

Nucleic Acid ◽

Acid Synthesis ◽

Nucleic Acid Synthesis ◽

Rat Diaphragm ◽

Isolated Rat

Download Full-text

Adrenal cortical hormone and incorporation of C14 from amino acid precursors into muscle protein

American Journal of Physiology-Legacy Content ◽

10.1152/ajplegacy.1960.198.2.360 ◽

1960 ◽

Vol 198 (2) ◽

pp. 360-362 ◽

Cited By ~ 19

Author(s):

Ira G. Wool ◽

Edward I. Weinshelbaum

Keyword(s):

Amino Acid ◽

Carboxylic Acids ◽

Muscle Protein ◽

Fold Change ◽

Rat Diaphragm ◽

Adrenal Cortical Hormone ◽

Amino Acid Precursors ◽

Isolated Rat ◽

Adrenalectomized Rats

The incorporation of C14 from labeled glucose, carboxylic acids and bicarbonate into the protein of isolated rat diaphragm was enhanced by adrenalectomy and decreased by cortisone treatment. The incorporation of radioactivity from pyruvate-3-C14 into the protein of diaphragms from adrenalectomized rats was not appreciably altered by a 20-fold change in pyruvate concentration. Incorporation of C14 from glucose-1-C14 and from glucose-6-C14 was the same, as was the magnitude of the increase after adrenalectomy.

Download Full-text

SENSITIVITY OF THE RAT DIAPHRAGM TO GROWTH HORMONE.

Acta Endocrinologica ◽

10.1530/acta.0.0540645 ◽

1967 ◽

Vol 54 (4) ◽

pp. 645-662 ◽

Cited By ~ 8

Author(s):

Å. Hjalmarson ◽

K. Ahrén

Keyword(s):

Growth Hormone ◽

Inhibitory Effect ◽

Rat Diaphragm ◽

Intracellular Accumulation ◽

Slight Effect ◽

Kidney Capsule ◽

Muscle Tissues ◽

Hypophysectomized Rats ◽

Isolated Rat

ABSTRACT The effect of growth hormone (GH) in vitro on the rate of intracellular accumulation of the non-utilizable amino acid α-aminoisobutyric acid (AIB) was studied in the intact rat diaphragm preparation. Bovine or ovine GH (25 μg/ml incubation medium) markedly stimulated the accumulation of AIB-14C by diaphragms from hypophysectomized rats, while there was no or only a very slight effect on diaphragms from normal rats. In diaphragms from rats with the pituitary gland autotransplanted to the kidney capsule GH in vitro stimulated the accumulation of AIB-14C significantly more than in diaphragms from normal rats but significantly less than in diaphragms from hypophysectomized rats. Injections of GH intramuscularly for 4 days to hypophysectomized rats made the diaphragms from these rats less sensitive or completely insensitive to GH in vitro. These results indicate strongly that the relative insensitivity to GH in vitro of diaphragms from normal rats is due to the fact that the muscle tissues from these rats has been exposed to the endogenously secreted GH. The results show that GH can influence the accumulation of AIB-14C in the isolated rat diaphragm in two different ways giving an acute or »stimulatory« effect and a late or »inhibitory« effect, and that it seems to be a time-relationship between these two effects of the hormone.

Download Full-text