scholarly journals PALLADIUM (II) CHLORIDE (PdCl2) SPECTROPHOTOMETRY TO DETERMINE LIPOIC ACID CONCENTRATION IN PLASMA AND LEUKOCYTES

2020 ◽  
Vol 3 (1) ◽  
pp. 14-22
Author(s):  
Novian Agni Yudhaswara ◽  
Ani Retno Prijanti ◽  
Mohamad Sadikin
Keyword(s):  
High Performance Liquid Chromatography ◽  
Lipoic Acid ◽  
Pyruvate Dehydrogenase ◽  
Body Fluid ◽  
High Performance ◽  
Chelating Agent ◽  
High Accuracy ◽  
Hplc Measurement ◽  
Acid Determination

Background: Lipoic acid is a substance contained in intra and extracellular that act as a coenzyme of Pyruvate Dehydrogenase, also as an antidote, chelating agent and antioxidant. Measurement of lipoic acid is needed to determine the amount of lipoic acid that performs its functions either as a coenzyme or an antioxidant. Besides, this measurement requires a special tool such as High Performance Liquid Chromatography (HPLC) and a process that is available in rural or simple laboratories.  Objective: A common and easy tool such as a spectrophotometer was conducted and could expected to be a tool of lipoic acid determination in body fluid such as plasma.Methods: Measurement of lipoic acid using spectrophotometry with UV methanol and visible PdCl2 has been tested and compared to HPLC measurement that was valid and reliable in drug measurement or pharmaceutical preparations.Results: Determination of lipoic acid in plasma and leukocytes using PdCl2 produced replicable, reliability and valid result, with high accuracy, precision and was not different from lipoic acid measurement using HPLC, p=0.99. While UV methanol was different compare to HPLC p =0.0001 or was not valid.Conclusion: The measurement of lipoic acid using PdCl2 visible method can be applied to determine the levels of lipoic acid (LA) and DHLA in plasma and equal to HPLC result.

scholarly journals Ionic liquid-based dispersive liquid-liquid microextraction combined with high performance liquid chromatography-UV detection for simultaneous preconcentration and determination of Ni, Co, Cu and Zn in water samples

2014 ◽  
Vol 79 (1) ◽  
pp. 63-76 ◽  
Author(s):  
Alireza Asghari ◽  
Mehri Ghazaghi ◽  
Maryam Rajabi ◽  
Mahdi Behzad ◽  
Mehrorang Ghaedi
Keyword(s):  
Ionic Liquid ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Water Samples ◽  
High Performance ◽  
Chelating Agent ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction ◽  
Cu And Zn

Ionic liquid-based dispersive liquid-liquid microextraction (IL-DLLME) coupled with high performance liquid chromatography (HPLC)-UV detection was developed for simultaneous extraction and determination of nickel, cobalt, copper and zinc ions. In the proposed approach, salophen (N,N'-bis(salisyliden)-1,2-phenylenediamine) was used as a chelating agent; the ionic liquid, 1-hexeyl-3-methylimidazolium hexafluorophosphate, and acetone were selected as extracting and dispersive solvents, respectively. After extraction, phase separation was performed by centrifugation and the sedimented phase (ionic liquid) was solubilized in acetonitrile and directly injected into the HPLC for subsequent analysis. Baseline separation of metal ion complexes was achieved on a RP-C18 column using a gradient elution of the mixtures of methanol-acetonitrile-water as the mobile phase at a flow rate of 1.0 mL min-1. The influence of variables such as sample pH, concentration of the chelating agent, amount of ionic liquid (extraction solvent), disperser solvent volume, extraction time, salt effect and centrifugation speed were studied and optimized. Under the optimum conditions, the enrichment factor of 222 was obtained. The detection limits for Ni, Co, Cu and Zn were 0.8, 1.6, 1.9 and 2.8 ?g L?1, respectively. The relative standard deviations (RSDs) were in the range of 3.6-5.0 % for all of the investigated metal ions. The proposed procedure was successfully applied to the determination of the studied metal ions in water samples.

scholarly journals Determination of lipoic acid in human plasma by high-performance liquid chromatography with ultraviolet detection

2019 ◽  
Vol 12 (8) ◽  
pp. 4878-4886 ◽  
Author(s):  
Grażyna Chwatko ◽  
Marta Krawczyk ◽  
Małgorzata Iciek ◽  
Adrianna Kamińska ◽  
Anna Bilska-Wilkosz ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Plasma ◽  
Lipoic Acid ◽  
High Performance ◽  
Ultraviolet Detection

Determination of lipoic acid by flow-injection and high-performance liquid chromatography with chemiluminescence detection

Talanta ◽  
2012 ◽  
Vol 96 ◽  
pp. 223-229 ◽  
Author(s):  
E. Wołyniec ◽  
J. Karpińska ◽  
S. Łosiewska ◽  
M. Turkowicz ◽  
J. Klimczuk ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Lipoic Acid ◽  
Flow Injection ◽  
High Performance ◽  
Chemiluminescence Detection
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