Clinical and functional aspects of cord blood cell transplantation in patients with distal lesion of lower limb arteries

To carry out a comparative analysis of the clinical condition of patients with distal lesions of the arterial bed using standard conservative therapy with cord blood cell transplantation and without it. The experimental group included patients with manifestations of chronic lower limb ischemia against the background of atherosclerosis, who underwent cord blood cell transplantation in combination with conservative therapy. The control group included patients with obliterating atherosclerosis and distal vascular lesions of the lower extremities, received a basic course of conservative therapy. To determine the quality of life before and after cord blood cell transplantation, we used a number of standardized questionnaires, which helped to determine the distance of intermittent claudication and make a subjective assessment of the personal well-being level of patients. To analyze the state of microcirculation in the ischemic limb, we used the laser Doppler flowmetry method. The following changes were observed after the cord blood cell transplantation: an improvement in the general state of patients, a decrease in the degree of ischemia according to Rutherford's classification, an increase in the distance and speed of painless walking during the first 3 months by 5-10%, and 1.3 times in 12 months compared with the control group. The improvement in the microcirculatory parameters of laser Doppler flowmetry was noted from the end of the first to the 12th month after transplantation, which indicated an improvement in microhemodynamics. The use of cord blood cell transplantation in order to stimulate angiogenesis allows one to obtain a long-term positive clinical effect, which manifests itself in the form of an increase in the distance and speed of painless walking, improved working capacity, and improved personal well-being of patients in the physical, psychological and socio-economic field, correlated with laser Doppler flowmetry data and expands the possibilities of successful treatment of patients with chronic ischemia of the lower extremities on the background of obliterating atherosclerosis.

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The response of skin perfusion and of rheological and immunological variables to intravenous prostanoid administration in Raynaud’s phenomenon secondary to collagenosis

VASA ◽

10.1024/0301-1526.34.4.243 ◽

2005 ◽

Vol 34 (4) ◽

pp. 243-249 ◽

Cited By ~ 2

Author(s):

Drinda ◽

Neumann ◽

Pöhlmann ◽

Vogelsang ◽

Stein ◽

...

Keyword(s):

Laser Doppler Flowmetry ◽

Raynaud’S Phenomenon ◽

Raynaud's Phenomenon ◽

Plasma Viscosity ◽

Laser Doppler ◽

Erythrocyte Aggregation ◽

Control Group ◽

Long Term Effects ◽

Doppler Flowmetry

Background: Prostanoids are used in the treatment of Raynaud’s phenomenon and acral perfusion disorders secondary to collagenosis. In subjective terms, intravenous administration of these agents produces success in more than 50% of patients. The therapeutic outcome of clinical administration of alprostadil or iloprost may vary from individual to individual. Patients and methods: The following variables were analysed in a cross-over study in 27 patients with collagenosis and Raynaud’s phenomenon: plasma viscosity and erythrocyte aggregation (rheological variables), partial pressure of oxygen and laser Doppler flowmetry in the finger region, and lymphocyte phenotyping and interleukin (IL) determinations (immunological variables). Results: Laser Doppler flowmetry revealed significant differences between patients with secondary Raynaud’s phenomenon and a control group of 25 healthy subjects. Laser Doppler readings did not change significantly as a result of the treatments. Therapy with iloprost produced a reduction in IL-1beta, L-selectin (CD 62 L) and IL-6. Conclusion: The change in immunological variables due to iloprost may explain the long-term effects of prostaglandins in the treatment of Raynaud’s phenomenon. From our results it is not possible to infer any preference for iloprost or alprostadil.

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Cord-Blood-Cell Transplantation — A Real Sleeper?

New England Journal of Medicine ◽

10.1056/nejm199502093320612 ◽

1995 ◽

Vol 332 (6) ◽

pp. 392-395 ◽

Cited By ~ 4

Author(s):

Robert Peter Gale

Keyword(s):

Blood Cell ◽

Cord Blood ◽

Cell Transplantation ◽

Cord Blood Cell

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Enhancement of Angiogenic Efficacy of Human Cord Blood Cell Transplantation

Tissue Engineering ◽

10.1089/ten.2006.12.1651 ◽

2006 ◽

Vol 12 (6) ◽

pp. 1651-1661 ◽

Cited By ~ 23

Author(s):

Seung-Woo Cho ◽

So-Jung Gwak ◽

Sun-Woong Kang ◽

Suk Ho Bhang ◽

Kang Won Song ◽

...

Keyword(s):

Blood Cell ◽

Cord Blood ◽

Cell Transplantation ◽

Human Cord Blood ◽

Cord Blood Cell

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Serum Cytokine Profile, Beta-Hexosaminidase A Enzymatic Activity and GM2 Ganglioside Levels in the Plasma of a Tay-Sachs Disease Patient after Cord Blood Cell Transplantation and Curcumin Administration: A Case Report

Life ◽

10.3390/life11101007 ◽

2021 ◽

Vol 11 (10) ◽

pp. 1007

Author(s):

Alisa A. Shaimardanova ◽

Daria S. Chulpanova ◽

Valeriya V. Solovyeva ◽

Ekaterina E. Garanina ◽

Ilnur I. Salafutdinov ◽

...

Keyword(s):

Blood Cell ◽

Enzymatic Activity ◽

Cord Blood ◽

Cell Transplantation ◽

Cytokine Profile ◽

Serum Cytokine ◽

Α Subunit ◽

Cord Blood Cell ◽

Tay Sachs Disease ◽

Hexosaminidase A

Tay-Sachs disease (TSD) is a progressive neurodegenerative disorder that occurs due to a deficiency of a β hexosaminidase A (HexA) enzyme, resulting in the accumulation of GM2 gangliosides. In this work, we analyzed the effect of umbilical cord blood cell transplantation (UCBCT) and curcumin administration on the course of the disease in a patient with adult TSD. The patient’s serum cytokine profile was determined using multiplex analysis. The level of GM2 gangliosides in plasma was determined using mass spectrometry. The enzymatic activity of HexA in the plasma of the patient was assessed using a fluorescent substrate assay. The HexA α-subunit (HexA) concentration was determined using ELISA. It was shown that both UCBCT and curcumin administration led to a change in the patient’s cytokine profile. The UCBCT resulted in an increase in the concentration of HexA in the patient’s serum and in an improvement in the patient’s neurological status. However, neither UCBCT nor curcumin were able to alter HexA activity and the level of GM2 in patient’s plasma. The data obtained indicate that UCBCT and curcumin administration can alter the immunity of a patient with TSD, reduce the level of inflammatory cytokines and thereby improve the patient’s condition.

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Efficacy of Laser Doppler Flowmetry, as a Diagnostic Tool in Assessing Pulp Vitality of Traumatised Teeth. A Split Mouth Clinical Study

Journal of Personalized Medicine ◽

10.3390/jpm11080801 ◽

2021 ◽

Vol 11 (8) ◽

pp. 801

Author(s):

Ani Belcheva ◽

Maria Shindova ◽

Reem Hanna

Keyword(s):

Laser Doppler Flowmetry ◽

Test Group ◽

Laser Doppler ◽

Permanent Teeth ◽

Control Group ◽

Eligibility Criteria ◽

Doppler Flowmetry ◽

Registration Number ◽

Pulp Vitality ◽

And Control

Aim: This study aimed to evaluate the efficacy of laser Doppler flowmetry (LDF) in determining the changes in the pulpal blood flow (PBF) during post-traumatic period of the traumatised permanent teeth. Methods: 88 teeth of 44 patients (mean age 10.30 ± 2.38) were recruited according to the eligibility criteria and divided into two groups: test group (44 traumatised teeth) and control group (44 sound and healthy teeth). The measurement of PBF was performed, using a LDF monitor. Results: The analysis of the LDF outcomes in function of diagnosis indicated that the measurements of the traumatised teeth were significantly higher than those of non-traumatised teeth (p ˂ 0.05). Conclusions: LDF application provides dentists with fundamental benefits in terms of an early and precise investigation of PBF. In addition, LDF is a useful monitoring tool for revascularization of traumatised teeth and reliable objective diagnostic indicator of pulp vitality. Trial registration: ClinicalTrials.gov (Registration number: NCT04967456).

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