scholarly journals ARTIFICIAL SEED PRODUCTION IN POINTED GOURD (TRICHOSANTHES DIOICA ROXB.)

2008 ◽  
Vol 21 (1) ◽  
pp. 15-20
Author(s):  
M. A. Malek ◽  
M. O. Islam ◽  
M. A. Bari Miah
Keyword(s):  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Formation ◽  
Nodal Segments ◽  
Conservation Strategies ◽  
Plantlet Formation ◽  
Artificial Seed ◽  
Pointed Gourd ◽  
Subsequent Regeneration

Encapsulation of nodal segments was successfully developed for pointed gourd towards the formation of artificial seed with sodium alginate. Encapsulated nodal segments (artificial seed) were cultured in MS basal medium containing different concentrations and combinations of BAP and NAA to induce germination and shoot proliferation. Highest (95%) shoot formation was obtained in MS + 1.0 mg/l BAP followed by MS + 0.5 mg/l BAP. The encapsulated nodal segments also regenerated in vitro on different substrates. Frequency of plantlet formation was low on these substrates compared to plantlet development on MS media. Among these substrates, the percentage of plantlet formation was better on moist cotton (42%) followed by filter paper (35%). The hardened plants were transferred successfully to soil in the earthen pots. The protocol for encapsulating the nodal segments for the production of artificial seeds and their subsequent regeneration is a new area of research to develop in vitro conservation strategies for pointed gourd.DOI: http://dx.doi.org/10.3329/bjpbg.v21i1.17044

scholarly journals In vitro propagation of pointed gourd (Trichosanthes dioica Roxb.) through encapsulated shoot tips

1970 ◽  
Vol 34 (4) ◽  
pp. 555-563 ◽  
Author(s):  
MA Malek
Keyword(s):  
Sodium Alginate ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Alginate Beads ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Shoot Cultures ◽  
Artificial Seed ◽  
Pointed Gourd

Plants were regenerated from encapsulated shoot tips of pointed gourd. Shoot tips isolated from multiple shoot cultures of AM-8 and AM-15 cultivars of pointed gourd were encapsulated in sodium alginate beads. For germination and shoot proliferation, encapsulated shoot tips (artificial seed) were cultured in MS basal medium containing different concentrations and combinations of BAP and NAA. Use of MS medium resulted in 90% conversion of encapsulated shoot tips into plantlets. The results exhibited that BAP and combinations of BAP and NAA play an important role in germination of artificial seed being encapsulated by sodium alginate beads. The plantlets were successfully established in earthen pot. Under the present study, limited experimental efforts have been made to establish the protocol for encapsulating the shoot tips for the production of artificial seed and their subsequent regeneration. It is the first report in Bangladesh in developing artificial seed production technique using vegetative tissue of pointed gourd. Key Words: In vitro propagation; pointed gourd; shoot tips. DOI: 10.3329/bjar.v34i4.5832Bangladesh J. Agril. Res. 34(4) : 555-563, December 2009

scholarly journals In vitro regeneration protocol for artificial seed production in an important medicinal plant Mentha arvensis L

2014 ◽  
Vol 20 ◽  
pp. 99-108 ◽  
Author(s):  
MS Islam ◽  
MA Bari
Keyword(s):  
Medicinal Plants ◽  
Seed Production ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Nodal Segments ◽  
Ms Medium ◽  
Mentha Arvensis ◽  
Artificial Seed ◽  
Artificial Seed Production

Context: The application of encapsulated shoot tips and nodal segments may contribute to the protection of rare and threatened medicinal plants. Although the artificial seed technique has been reported for more than two decades, for medicinal plants this method has not been developed sufficiently. The main limitations in conventional propagation of some species with medicinal value are: reduced endosperm, low germination rate and seedless varieties. The above mentioned reasons indicate the need for the production of artificial seeds as a technique which combines the advantages of clonal multiplication with those of seed propagation and storage. Objectives: The objective of the present investigation was to standardize artificial seed production technology taking shoot tip and nodal explants in Mentha arvensis and its in vitro regeneration Materials and Methods: Sodium alginate beads were produced by encapsulation of shoot tip and nodal segments of the plant M. arvensis. MS medium was used as basal medium with agar and sodium alginate was used as gelling agent accompanied by CaCl2 solution. Results: Different concentrations and combinations of BAP, Kin and NAA were used in alginate bead in MS basal medium. Among the different concentrations of phytohormone, highest 80% of shoot formation was observed in MS medium containing 2.0 mg/l BAP + 0.2 mg/l NAA from nodal segments of M. arvensis. Highest average number of shoot 9.87 ± 0.58 formation was obtained in the same medium but highest length of shoot 6.27 ± 0.29 cm was found in the medium having 1.0 mg/l BAP + 0.5 mg/l NAA. Conclusion: The present investigation clearly established and demonstrated the method of obtaining the artificial seed production in M. arvensis supported by different hormone concentrations DOI: http://dx.doi.org/10.3329/jbs.v20i0.17722 J. bio-sci.  20:  99-108, 2012

scholarly journals In Vitro Propagation of Muscadine Grape by Axillary Shoot Proliferation

1990 ◽  
Vol 115 (2) ◽  
pp. 324-329 ◽  
Author(s):  
Ni Lee ◽  
Hazel Y. Wetzstein
Keyword(s):  
Basal Medium ◽  
Shoot Proliferation ◽  
Nodal Segments ◽  
Axillary Shoot ◽  
Axillary Shoot Proliferation ◽  
Vitis Rotundifolia ◽  
Shoot Production ◽  
Muscadine Grape ◽  
Better Than

Plantlets were recovered from axillary bud cultures of muscadine grape (Vitis rotundifolia, `Summit'). Nodal segments 0.5 to 1.0 cm long were cultured in Murashige and Skoog (MS) basal medium supplemented with 5, 10, 20, or 40 μm BA. Best total shoot production was obtained with 10 μm BA; with higher BA levels, shoots were unexpanded and exhibited high mortalities. MS medium supplemented with IBA enhanced rooting by increasing rooting percentage and number per plantlet. Shoots previously proliferated on medium with 5 μm BA rooted significantly better than those multiplied on 10 μM BA. Shoot vigor during rooting was greater in shoots proliferated on 5 vs. 10 μm BA. Root development was not significantly affected by liquid vs. agar-solidifted medium or shoot length. Chemical names used: N-(phenylmethyl) -1H-purin-6-amine (BA), 1H-indole-3-butyric acid (IBA).

scholarly journals Micropropagation of Turbinicarpus valdezianus (Möeller) Glass & Foster (Cactaceae) an Endemic Cactus in Northern Mexico

HortScience ◽  
2016 ◽  
Vol 51 (1) ◽  
pp. 94-97 ◽  
Author(s):  
Alejandro Martínez Palacios ◽  
Raúl Cárdenas Navarro ◽  
Diana Beatriz Hernández Ortega ◽  
Víctor Chávez Avila
Keyword(s):  
Clonal Propagation ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Inhibitory Effect ◽  
Multiplication Rate ◽  
Adventitious Shoot Formation ◽  
In Vitro Clonal Propagation

An in vitro clonal propagation protocol based on axillary bud development was generated for Turbinicarpus valdezianus. An efficient multiplication rate was obtained using either longitudinal or apical explants from in vitro germinated seedlings. The proliferation capacity of these explants was evaluated by testing the single and interaction effects of five concentrations of 6-furfurylaminopurine (KIN) (0.00, 2.32, 4.64, 9.28, and 18.56 µm) and three concentrations of α-naphthalenacetic acid (NAA) (0.00, 0.54, and 2.70 µm), using Murashige and Skoog (MS) as basal medium. Statistical analysis showed that the highest average shoot proliferation of T. valdezianus was recorded with 9.28 µm of KIN, producing 11.75 and 4.50 plantlets per initial explant, for apical and lateral explants, respectively. Addition of NAA to the medium had an inhibitory effect on shoot proliferation for both explant types. The developed shoots in 9.28 µm of KIN and plant growth regulator (PGR)-free treatments were used for a rooting subculture phase. These shoots were then transferred to PGR-free MS medium, resulting in statistically significant different rooting frequencies of 78% and 97%, respectively. When transplanted in soil, the rooted shoots showed an average survival rate of 90%, without any significant statistical differences between treatments. This propagation protocol has the capacity to produce near to 21 plantlets per seedling in 27 weeks, i.e., 11.78 and 9.00 plantlets per apical and lateral explants, respectively, without callus or adventitious shoot formation. These features made it highly attractive as an in vitro clonal propagation method for T. valdezianus plants and the later implementation of a rescue program for threatened wild populations of this cacti species.

scholarly journals Callus Induction and High Frequency Regeneration of Plantlets of Scoparia dulcis L., a Perennial Medicinal Herb, Through Auxiliary Shoot Proliferation

1970 ◽  
Vol 18 (1) ◽  
pp. 75-83 ◽  
Author(s):  
A.K.M. Sayeed Hassan ◽  
Farhana Afroz ◽  
Laila Shamroze Bari ◽  
John Liton Munshi ◽  
Miskat Ara Akter Jahan ◽  
...  
Keyword(s):  
Leaf Shape ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Medicinal Herb ◽  
Nodal Segments ◽  
Scoparia Dulcis ◽  
Regenerated Plants ◽  
Nodular Callus ◽  
Half Strength

Green compact nodular callus was observed within three weeks from nodal segments of a perennial medicinal herb Scoparia dulcis L. on MS basal medium supplemented with 1.5 mg/l BAP + 0.2 mg/I NAA. The callus produced large number of shoots when subcultured on MS with 0.5 mg/l BAP + 0.1 mg/l NAA. In vitro raised shoots rooted on half strength of MS with 1.0 mg/l IBA + 1.0 mg/l NAA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for seven days before transplanting in pots where plantlets were reared for three weeks. The survival rate of plantlets was found to be 85%. Regenerated plants were morphologically uniform having normal leaf shape and growth. Key words: Scoparia dulcis, Medicinal plant, Shoot proliferation, Acclimatization D.O.I. 10.3329/ptcb.v18i1.3268 Plant Tissue Cult. & Biotech. 18(1): 75-83, 2008 (June)

scholarly journals Effect of Culture Media and Plant Growth Regulators on Shoot Proliferation and Rooting of Internode Explants from Moroccan Native Almond (Prunus dulcis Mill.) Genotypes

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Souhayla Kodad ◽  
Reda Melhaoui ◽  
Christophe Hano ◽  
Mohamed Addi ◽  
Nargis Sahib ◽  
...  
Keyword(s):  
Culture Media ◽  
Shoot Proliferation ◽  
Shoot Formation ◽  
Prunus Dulcis ◽  
Nodal Segments ◽  
Ms Medium ◽  
In Vitro Proliferation ◽  
Eastern Morocco

In this study, several methods have been used to facilitate shoot formation from nodal explants of local almond ecotypes known as “Beldi” grown in Eastern Morocco. Nodal segments of divers old local genotypes were cultured on various concentrations of auxin (indole-3-butyric acid (IBA)) and cytokinins (6-benzyl-aminopurine (BAP), thidiazuron (TDZ), and kinetin (KIN)) added to two different media (Murashige and Skoog (MS) and Heller medium). The results showed that TDZ was more effective than the other tested hormones for in vitro proliferation of the “Beldi” ecotype. TDZ at the concentration of 1 mg/L significantly improved the nodal shoot proliferation rate, with the highest percentage (63.6% ± 0.63) and number of regenerated shoots (13 ± 0.54) recorded for S1 genotype inoculated on MS medium, while the most significant rooting rate (60.41% ± 0.81) of proliferated shoots and number of roots per shoot (7.3 ± 1.36) were achieved for S2 genotype on 1 mg/L of IBA incorporated to a half-strength MS medium. With 80% of plantlets survival, the rooted shoots were successfully adapted to the in vivo conditions and were grown vigorously in the greenhouse without any morphological abnormalities.

scholarly journals In Vitro Regeneration of Trichosanthes from Shoot Tips

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 871C-871 ◽  
Author(s):  
U.L. Yadava ◽  
S.K. Dhir
Keyword(s):  
In Vitro Regeneration ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Normal Growth ◽  
Multiple Shoots ◽  
Shoot Formation ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Pointed Gourd

The morphogenetic potential of parval or pointed gourd (Trichosanthes dioica Roxb.) shoot-tip explants was investigated to establish this species as a model tissue culture system. An effective multiple-shoot propagation method is described. Ten-millimeter shoot tips from young branches of greehouse-grown plants served as explants. They were initiated on a MS basal medium. Multiple shoots were encouraged by transferring established explants to a proliferation medium consisting of MSB + 1 mg BAP/liter, because lower concentrations of BAP (0.1 to 0.5 mg–liter–1) inhibited multiple shoot formation; however, the same concentrations promoted rooting in explants. Medium supplemented with 1 mg BAP/liter and 100 mg PVP/liter caused the best proliferation of shoot tips. Upon transferring to fresh medium of the same composition, these shoot tips elongated 24 cm with three to five nodes in 4 weeks of culturing. Shoot multiplication cultures were maintained by transferring segments of multiple-shoot clusters to medium containing 1 mg BAP/liter and 0.5 mg GA3/liter. Medium supplemented with TDZ inhibited the number of regenerating explants but enhanced the number of shoot buds. Eighty percent of these plantlets were successfully rooted on MS medium supplemented with 1 mg NAA/liter. Plantlets survived in potting soil and exhibited normal growth under mist in the greenhouse.

scholarly journals In Vitro Micropropagation of the Medicinal Plant Physalis angulata L.

2016 ◽  
Vol 8 (2) ◽  
pp. 161-163
Author(s):  
Owk ANIEL KUMAR ◽  
Songa RAMESH ◽  
Sape SUBBA TATA
Keyword(s):  
Large Scale ◽  
Leaf Disc ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Medicinal Herb ◽  
Root Induction ◽  
Survival Percentage ◽  
Physalis Angulata

Physalis angulata L. is an important medicinal herb. An efficient direct adventitious plant regeneration protocol was developed for large scale propagation using leaf disc as explants. The explants were cultured on MS basal medium supplemented with 0.25-3.0 mg/L 6-benzyl amino purine (BAP) for primary shoot proliferation. Inclusion of indole-3-acetic acid (IAA) and gibberellic acid (GA3) in the culture medium along with BAP promoted a higher rate of shoot multiplication. The maximum number of shoots was produced in MS + BAP (1.0 mg/L) + IAA (0.5 mg/L) + GA3 (0.20 mg/L) after the third subculture. An average of 152.8 ± 0.40 shoots were produced from each leaf disc. For root induction the shootlets were transferred to MS medium supplemented with different concentrations of indole-3-butyric acid (IBA). The highest percentage of root induction was observed in 1.0 mg/L (IBA). Rooted plants were successfully established in the soil after hardening. The survival percentage of rooted plants on soil was found to be 85%. This result will facilitate the conservation and propagation of the important medicinal herb Physalis angulata L.

scholarly journals Media composition affects seed dormancy, apical dominance and phenolic profile of Knautia sarajevensis (Dipsacaceae), Bosnian endemic

2018 ◽  
Vol 77 (1) ◽  
pp. 70-79 ◽  
Author(s):  
Erna Karalija ◽  
Sanja Ćavar Zeljković ◽  
Petr Tarkowski ◽  
Edina Muratović ◽  
Adisa Parić
Keyword(s):  
Apical Dominance ◽  
Germination Rate ◽  
Natural Habitat ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Mineral Nutrient ◽  
Total Phenolic ◽  
Endemic Plant ◽  
High Concentrations

AbstractKnautia sarajevensisis an endemic plant of the Dinaric Alps and is mainly distributed on Bosnian Mountains. Due to the quite large flower heads and easy maintenance, this plant has a potential use as a substitute ornamental plant forK. arvensisin perennial beds. The current study evaluated the germination process in different treatments in an attempt to suppress dormancy and increase germination rate, and to develop a successful protocol for micropropagation. An over 60% germination rate was achieved through cultivation of seeds on MS basal medium with reduced mineral nutrient composition and the absence of sucrose. On the other hand, a below 10% germination rate was achieved with untreated seeds. Suppression of apical dominance was achieved through application of high concentrations of kinetin, apical shoot decapitation or cultivation of shoots in liquid media. Overall, liquid cultures were more successful as a micropropagation system for this plant. Shoots spontaneously developed roots on multiplication treatments and were successfully acclimatized. Moreover, phenolic compound profile was analysed in the light of the possible medicinal potential of this plant. Variable amounts of total phenolic compounds as well as individual phenolics were recorded, according to treatment and solidification of media. An increase in rosmarinic acid content was reported for kinetin treatments and acclimatized plants comparing to mother plants in natural habitat. The present study shows that choice of cytokinin concentration, explant type as well as culture type influences not only shoot proliferation and apical dominance suppression but alsoin vitroproduction of phenolics.

scholarly journals In vitro Regeneration of the Medicinal Plant, Plumbago zeylanica L. with Reference to a Unique Population in Maruthamalai, the Western Ghats, India

1970 ◽  
Vol 18 (2) ◽  
pp. 173-179 ◽  
Author(s):  
T. Mallikadevi ◽  
P. Senthilkumar ◽  
S. Paulsamy
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Garden Soil ◽  
Plumbago Zeylanica ◽  
Adventitious Shoot Formation ◽  
The Western Ghats

The in vitro regeneration of Plubago zeylanica exhibited that the callus was initiated in the basal medium containing BAP, NAA, 2, 4-D, and IBA.  The high amount (90%) of organic calli was induced in the basal medium supplemented with 2, 4-D, alone at 2.0 mg/l. In the subculture the adventitious shoot formation was prominently higher (83%) in the basal medium containing BAP, and NAA at 3.5 and 0.3 mg/l, respectively. IAA (1.0 mg/l)effectively produced higher percen-tage (90) of roots and root growth. After sequential hardening, survivability rate was observed to be significantly higher (80%) in the hardening medium containing garden soil, sand and vermicompost in the ratio of 1 : 1 : 1 by volume under greenhouse condition.  Key words: Plumbago zeylanica, In vitro regeneration, Medicinal plant D.O.I. 10.3329/ptcb.v18i2.3648 Plant Tissue Cult. & Biotech. 18(2): 173-179, 2008 (December)

Sign in / Sign up

Export Citation Format

Share Document