scholarly journals In Vitro Mass Propagation of Mimosa pudica L., Using Shoot Tip and Nodal Explants

1970 ◽  
Vol 45 (2) ◽  
pp. 95-100 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
Rebeka Sultana ◽  
Miskat Ara Akhter Jahan ◽  
Rahima Khatun
Keyword(s):  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Shoot Tip ◽  
Nodal Explants ◽  
Mimosa Pudica ◽  
Mass Propagation ◽  
Half Strength ◽  
Proliferation In Vitro

An efficient protocol was established for in vitro mass propagation of a valuable medicinal shrubby plant, Mimosa pudica Linn., from shoot tip and nodal explants. Optimum in vitro shoot induction was observed from nodal explants on MS basal medium supplemented with 1.5 mg/l BAP + 0.5 mg/l NAA, in which 88.2% of the explants produced 9 shoots per culture within 3-4 weeks. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 20.4 ± 1.20 shoots per culture within 12 weeks. The healthy in vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IBA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 80%. Key words: Mimosa pudica; Medicinal plant; Shoot proliferation; In vitro mass propagation; Acclimatization DOI: 10.3329/bjsir.v45i2.5704Bangladesh J. Sci. Ind. Res. 45(2), 95-100, 2010

scholarly journals Micropropagation of Eclipta alba (Linn.) Hassk- a Valuable Medicinal Herb

1970 ◽  
Vol 43 (2) ◽  
pp. 215-222 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
Farhana Afroz ◽  
Laila Shamroze Bari ◽  
John Liton Munshi ◽  
Miskat Ara Akhter Jahan ◽  
...  
Keyword(s):  
Room Temperature ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Medicinal Herb ◽  
Mass Propagation ◽  
Eclipta Alba ◽  
Half Strength ◽  
Regenerated Plantlets

A protocol was established for mass propagation of a valuable medicinal herb, Eclipta alba (Linn.) Hassk (Asteraceae) through in vitro culture. Apical and axillary buds of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mgl-1 BAP + 0.1 mgl-1 NAA, in which 94% of the explants produced 18 shoots per culture. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 26 shoots per culture. In vitro raised shoots rooted on half strength MS medium with 1.0 mgl-1 IBA +1.0 mgl-1 NAA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 80%. Key words: Eclipta alba, Medicinal plant, Shoot proliferation, Micropropagation, Acclimatization   DOI: 10.3329/bjsir.v43i2.965 Bangladesh J. Sci. Ind. Res. 43(2), 215-222, 2008 

scholarly journals In Vitro Mass Propagation of Heliotropium indicum L., using Apical and Axillary Bud Explants

1970 ◽  
Vol 45 (1) ◽  
pp. 69-74 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
Nadira Begum ◽  
Miskat Ara Akhter Jahan ◽  
Rahima Khatun
Keyword(s):  
Room Temperature ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Axillary Buds ◽  
Mass Propagation ◽  
Half Strength ◽  
Heliotropium Indicum ◽  
Regenerated Plantlets

A consequency was obtained for mass propagation of a valuable ayurvedic medicinal herb, Heliotropium indicum Linn. (Boraginaceae) through in vitro culture. Apical and axillary buds of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP + 0.1 mg/l GA3, in which 92% of the axillary buds explants produced 12 shoots per culture. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 18 shoots per culture. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IBA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. Key words: Heliotropium indicum; Medicinal plant; Shoot proliferation; Micropropagation; Acclimatization. DOI: 10.3329/bjsir.v45i1.5185 Bangladesh J. Sci. Ind. Res. 45(1), 69-74, 2010

scholarly journals In vitro Mass Propagation of a Ground Orchid - Phaius tancarvilleae (L'Her.) Blume through Shoot Tip Culture

2011 ◽  
Vol 21 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Bijaya Pant ◽  
Sumitra Shrestha
Keyword(s):  
High Frequency ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Shoot Tip ◽  
Mass Propagation ◽  
Shoot Tip Culture ◽  
Shoot Tip Explants ◽  
Direct Shoot

High frequency direct shoot proliferation was induced from the shoot tip explants derived from the in vitro grown seedlings of a critically endangered and horticulturally important ground orchid Phaius tancarvilleae (L'Her) Blume. Shoot tip explants cultured on solidified MS with alone or combination of various concentrations of NAA and BAP produced shoots and multiple shoots. The maximum number of healthy shoots was observed on MS with BAP (1.0 mg/l) with an average of 13.3 shoots per culture in 20 weeks; where shoot multiplication was initiated after 4 weeks of culture. Regenerated shoots rooted on MS with various concentrations of NAA, IAA, IBA. MS with NAA (0.5 mg/l) was the most appropriate condition for rooting. The well developed in vitro rooted plantlets were hardened successfully in the potting mixture containing cocopeat and sphagnum moss in the ratio of 2 : 1.   Key words: Mass propagation, Phaius tancarvilleae, shoot multiplication   D. O. I. 10.3329/ptcb.v21i2.10241   Plant Tissue Cult. & Biotech. 21(2): 181-188, 2011 (December)

scholarly journals In vitro Regeneration through Apical and Axillary Shoot Proliferation of Ficus religiosa L. - A Multi-purpose Woody Medicinal Plant

2010 ◽  
Vol 19 (1) ◽  
pp. 71-78 ◽  
Author(s):  
A.K.M. Sayeed Hassan ◽  
Farhana Afroz ◽  
Miskat Ara Akhter Jahan ◽  
Rahima Khatun
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Axillary Shoot ◽  
Axillary Shoot Proliferation ◽  
Ficus Religiosa ◽  
Half Strength

A protocol was established for mass propagation of the valuable medicinal plant Ficus religiosa L. (Moraceae) through in vitro culture using apical and axillary buds of young sprouts from selected plants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP + 0.1 mg/l IAA, in which 78 per cent of the explants produced 16 shoots per culture. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 24 shoots per culture. In vitro raised shoots rooted on half strength MS supplemented with 2.0 mg/l IBA + 0.1 mg/l NAA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for seven days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85 per cent.  Key words: Ficus religiosa, Medicinal plant, Shoot proliferation, Regeneration,                   Acclimatization D.O.I. 10.3329/ptcb.v19i1.4987 Plant Tissue Cult. & Biotech. 19(1): 71-78, 2009 (June)

scholarly journals 027 MULTIPLICATION OF ROSE SPECIES IN VITRO

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 431d-431
Author(s):  
Yan Ma ◽  
David H. Byrne ◽  
Jing Chen ◽  
Amanda Byrne
Keyword(s):  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Naphthalene Acetic Acid ◽  
Good Growth ◽  
Lateral Buds ◽  
Half Strength ◽  
The Media

Several rose species (Rosa rugosa, R. wichuraiana, R. setigera, R. laevigata, R. banksiae, R. roxburghii, R. odorata and hybrids) were employed to establish the appropriate nutrient media for shoot multiplication and root initiation of cultured shoots and to describe a procedure for the successful transfer to soil of plants obtained in vitro. Cultured shoot tips and lateral buds from different genotypes proliferated multiple shoots on a basal medium (MS salt, vitamins, glycine, sucrose and agar) supplemented with 0mg/l to 6mg/l 6-benzylamino purine (BA) and 0mg/l to 0.5 mg/l naphthalene acetic acid (NAA). Most rose species cultured in a modified MS medium supplemented with 2mg/l BA showed good growth and shoot proliferation. The buds nearest the apex exhibited the slowest rate of bud development. Root development was enhanced and shoot development inhibited by lowering the concentration of MS salts to quarter- and half-strength. With difficult-to-root species, rooting was improved by supplementing the media with auxin or giving them 3-7days of dark treatment.

scholarly journals In vitro mass propagation of Plumbago zeylanica L., through direct organogenesis

2012 ◽  
Vol 47 (3) ◽  
pp. 297-302
Author(s):  
AKMS Hassan ◽  
F Haque ◽  
MAA Jahan ◽  
SK Roy
Keyword(s):  
Room Temperature ◽  
Basal Medium ◽  
Nodal Explants ◽  
Direct Organogenesis ◽  
Shoot Induction ◽  
Mass Propagation ◽  
Plumbago Zeylanica ◽  
Half Strength ◽  
Regenerated Plantlets

An efficient protocol was developed for in vitro mass propagation of an important medicinal shrub, Plumbago zeylanica L., (Plumbaginaceae) through direct organogenesis using shoot tip and nodal explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP, in which 86.4% of nodal explants responded to produce maximum number (12.4 ± 0.66) of shoots per culture. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IAA. For acclimatization and transplantation, the plantlets in the rooted culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. DOI: http://dx.doi.org/10.3329/bjsir.v47i3.13063 Bangladesh J. Sci. Ind. Res. 47(3), 297-302 2012

scholarly journals In Vitro Shoot Proliferation and Plant Regeneration of Phyllanthus fraternus Webster (B. Bhuiamla), a Seasonal Medicinal Herb

1970 ◽  
Vol 46 (2) ◽  
pp. 205-210 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
R Khatun
Keyword(s):  
Plant Regeneration ◽  
Large Scale ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Medicinal Herb ◽  
Nodal Explants ◽  
Efficient System ◽  
Half Strength ◽  
Phyllanthus Fraternus

An efficient system was developed for shoot proliferation and large scale plant regeneration of a seasonal multipotent medicinal herb, Phyllanthus fraternus Webster through in vitro culture. Shoot tips and nodal explants of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP + 0.1 mg/l GA3, in which 88% of nodal explants responded to produce maximum number (16.8 ± 0.95) of shoots per culture. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IBA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 82%. Key words: Phyllanthus fraternus; Medicinal plant; Shoot proliferation; Regeneration; Acclimatization DOI: http://dx.doi.org/10.3329/bjsir.v46i2.8187 Bangladesh J. Sci. Ind. Res. 46(2), 205-210, 2011

scholarly journals In Vitro Clonal Propagation of Scoparia dulcis L., a Perennial Medicinal Herb

1970 ◽  
Vol 44 (3) ◽  
pp. 341-346
Author(s):  
AKM Sayeed Hassan ◽  
Laila Shamroze Bari ◽  
Rebeka Sultana ◽  
Nadira Begum ◽  
Rahima Khatun
Keyword(s):  
Clonal Propagation ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Medicinal Herb ◽  
Naphthaleneacetic Acid ◽  
Scoparia Dulcis ◽  
Half Strength ◽  
In Vitro Clonal Propagation

An efficient protocol was established for in vitro clonal propagation of the perennial medicinal herb Scoparia dulcis L. (Family. Scrophulariaceae) through in vitro culture. Apical and axillary buds of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.1 mg/l BAP, in which 94% of the explants produced 12 shoots per culture. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 16 shoots per culture. The half strength MS medium with 0.5 mg/l IBA +0.5 mg/l NAA the highest percentage (85.20) and maximum number (13.40) of roots were initiated within four weeks of culture. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. Key words: Scoparia dulcis, Medicinal plant, Shoot proliferation, Micropropagation, Acclimatization, IAA (indoleacetic acid), IBA(indolebutanoic acid), NAA(α-naphthaleneacetic acid), BAP(benzylamino purine) DOI: 10.3329/bjsir.v44i3.4408 Bangladesh J. Sci. Ind. Res. 44(3), 341-346, 2009

scholarly journals In vitro shoot multiplication of the biodiesel plant Jatropha curcas L. through direct organogenesis

2014 ◽  
Vol 49 (1) ◽  
pp. 41-46 ◽  
Author(s):  
MAKMS Hassan ◽  
N Begum ◽  
LS Bari ◽  
MAA Jahan
Keyword(s):  
Jatropha Curcas ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Nodal Explants ◽  
Direct Organogenesis ◽  
Shoot Induction ◽  
Jatropha Curcas L ◽  
Half Strength ◽  
Regenerated Plantlets

An efficient protocol was established for in vitro shoot multiplication of the biodiesel plant, Jatropha curcas L. (Euphorbiaceae) through direct organogenesis using shoot tip and nodal explants. Best shoot induction was observed on MS basal medium supplemented with 1.5 mg/l BAP + 0.5 mg/l NAA, in which 86.2% of nodal explants responded to produce maximum number (7.2 ± 0.68) of shoots per culture. In vitro raised shoots rooted on half strength MS medium with 1.0 mg/l IAA. The survival rate of regenerated plantlets was 85%. DOI: http://dx.doi.org/10.3329/bjsir.v49i1.18854 Bangladesh J. Sci. Ind. Res. 49(1), 41-46, 2014

scholarly journals MICROPROPAGATION OF WHITE EASTERN REDBUD (CERCIS CANADENSIS VAR. ALBA.

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1091b-1091 ◽  
Author(s):  
S. Yusnita ◽  
Robert L. Geneve ◽  
Sharon T. Kester
Keyword(s):  
Adventitious Shoots ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Shoot Tip ◽  
Coarse Roots ◽  
Cercis Canadensis ◽  
Half Strength ◽  
Eastern Redbud ◽  
Shoot Tip Necrosis

In vitro shoot multiplication of white Eastern redbud was successful using two-node mature explants from the initial spring flush on a woody plant medium (WPM) supplemented with benzylaminopurine (BAP). Optimal shoot proliferation was obtained at 10-15 μM BAP. Treatment with thidiazuron produced fasciated (stunted) adventitious shoots which failed to elongate. Successive subcultures increased the ability of explants to form shoots. However, shoot tip necrosis became a problem after 7-8 subcultures. Shoot tip necrosis is being studied by comparing shoot multiplication on bacto-agar vs. gelrite, increasing the Ca concentration in WPM and by trying to reduce the phenolic exudate by the explants with PVP or activated charcoal. Microshoots >3 cm long were rooted by pulse treatment on half strength WPM containing 300 μM IBA or NAA before being moved to hormone free WPM. There was a different morphology between IBA and NAA induced roots, although the number of roots were comparable. IBA treated microcuttings developed branched, fine roots, whereas NAA treated plants produced unbranched, coarse roots. Rooted microshoots were successfully acclimated to greenhouse condition.

Sign in / Sign up

Export Citation Format

Share Document