scholarly journals In Vitro Shoot Proliferation and Plant Regeneration of Phyllanthus fraternus Webster (B. Bhuiamla), a Seasonal Medicinal Herb

1970 ◽  
Vol 46 (2) ◽  
pp. 205-210 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
R Khatun
Keyword(s):  
Plant Regeneration ◽  
Large Scale ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Medicinal Herb ◽  
Nodal Explants ◽  
Efficient System ◽  
Half Strength ◽  
Phyllanthus Fraternus

An efficient system was developed for shoot proliferation and large scale plant regeneration of a seasonal multipotent medicinal herb, Phyllanthus fraternus Webster through in vitro culture. Shoot tips and nodal explants of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP + 0.1 mg/l GA3, in which 88% of nodal explants responded to produce maximum number (16.8 ± 0.95) of shoots per culture. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IBA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 82%. Key words: Phyllanthus fraternus; Medicinal plant; Shoot proliferation; Regeneration; Acclimatization DOI: http://dx.doi.org/10.3329/bjsir.v46i2.8187 Bangladesh J. Sci. Ind. Res. 46(2), 205-210, 2011

scholarly journals In Vitro Micropropagation of the Medicinal Plant Physalis angulata L.

2016 ◽  
Vol 8 (2) ◽  
pp. 161-163
Author(s):  
Owk ANIEL KUMAR ◽  
Songa RAMESH ◽  
Sape SUBBA TATA
Keyword(s):  
Large Scale ◽  
Leaf Disc ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Medicinal Herb ◽  
Root Induction ◽  
Survival Percentage ◽  
Physalis Angulata

Physalis angulata L. is an important medicinal herb. An efficient direct adventitious plant regeneration protocol was developed for large scale propagation using leaf disc as explants. The explants were cultured on MS basal medium supplemented with 0.25-3.0 mg/L 6-benzyl amino purine (BAP) for primary shoot proliferation. Inclusion of indole-3-acetic acid (IAA) and gibberellic acid (GA3) in the culture medium along with BAP promoted a higher rate of shoot multiplication. The maximum number of shoots was produced in MS + BAP (1.0 mg/L) + IAA (0.5 mg/L) + GA3 (0.20 mg/L) after the third subculture. An average of 152.8 ± 0.40 shoots were produced from each leaf disc. For root induction the shootlets were transferred to MS medium supplemented with different concentrations of indole-3-butyric acid (IBA). The highest percentage of root induction was observed in 1.0 mg/L (IBA). Rooted plants were successfully established in the soil after hardening. The survival percentage of rooted plants on soil was found to be 85%. This result will facilitate the conservation and propagation of the important medicinal herb Physalis angulata L.

scholarly journals In vitro Shoot Proliferation and Plant Regeneration of Phlogacanthus thyrsiflorus Nees. a Rare Medicinal Shrub of Bangladesh

2011 ◽  
Vol 21 (2) ◽  
pp. 135-141 ◽  
Author(s):  
A.K. M. Sayeed Hassan ◽  
Nadira Begum ◽  
Rebeka Sultana ◽  
Rahima Khatun
Keyword(s):  
Survival Rate ◽  
Plant Regeneration ◽  
Room Temperature ◽  
Shoot Proliferation ◽  
Nodal Explants ◽  
Shoot Induction ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
In Vitro Shoot Proliferation

An efficient protocol was developed for shoot proliferation and plant regeneration of Phlogacanthus thyrsiflorus Nees. (Acanthaceae) - a rare medicinal shrub of Bangladesh, through in vitro culture using shoot tip and nodal explants. Best shoot induction was observed on MS with 1.0 mg/l BAP + 0.5 mg/l NAA, in which 84.2% of nodal explants responded to produce maximum number (12.4 ± 0.66) of shoots per culture. In vitro raised shoots rooted on half-strength MS with 0.5 mg/l IBA + 0.5 mg/l NAA. For acclimation and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. Key words: Phlogacanthus thyrsiflorus, Shoot proliferation, Plant regeneration   D. O. I. 10.3329/ptcb.v21i2.10236   Plant Tissue Cult. & Biotech. 21(2): 135-141, 2011 (December)

scholarly journals Micropropagation of Eclipta alba (Linn.) Hassk- a Valuable Medicinal Herb

1970 ◽  
Vol 43 (2) ◽  
pp. 215-222 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
Farhana Afroz ◽  
Laila Shamroze Bari ◽  
John Liton Munshi ◽  
Miskat Ara Akhter Jahan ◽  
...  
Keyword(s):  
Room Temperature ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Medicinal Herb ◽  
Mass Propagation ◽  
Eclipta Alba ◽  
Half Strength ◽  
Regenerated Plantlets

A protocol was established for mass propagation of a valuable medicinal herb, Eclipta alba (Linn.) Hassk (Asteraceae) through in vitro culture. Apical and axillary buds of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mgl-1 BAP + 0.1 mgl-1 NAA, in which 94% of the explants produced 18 shoots per culture. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 26 shoots per culture. In vitro raised shoots rooted on half strength MS medium with 1.0 mgl-1 IBA +1.0 mgl-1 NAA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 80%. Key words: Eclipta alba, Medicinal plant, Shoot proliferation, Micropropagation, Acclimatization   DOI: 10.3329/bjsir.v43i2.965 Bangladesh J. Sci. Ind. Res. 43(2), 215-222, 2008 

scholarly journals In Vitro Clonal Propagation of Scoparia dulcis L., a Perennial Medicinal Herb

1970 ◽  
Vol 44 (3) ◽  
pp. 341-346
Author(s):  
AKM Sayeed Hassan ◽  
Laila Shamroze Bari ◽  
Rebeka Sultana ◽  
Nadira Begum ◽  
Rahima Khatun
Keyword(s):  
Clonal Propagation ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Medicinal Herb ◽  
Naphthaleneacetic Acid ◽  
Scoparia Dulcis ◽  
Half Strength ◽  
In Vitro Clonal Propagation

An efficient protocol was established for in vitro clonal propagation of the perennial medicinal herb Scoparia dulcis L. (Family. Scrophulariaceae) through in vitro culture. Apical and axillary buds of young sprouts from selected plants were used as explants. Best shoot induction was observed on MS basal medium supplemented with 0.1 mg/l BAP, in which 94% of the explants produced 12 shoots per culture. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 16 shoots per culture. The half strength MS medium with 0.5 mg/l IBA +0.5 mg/l NAA the highest percentage (85.20) and maximum number (13.40) of roots were initiated within four weeks of culture. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. Key words: Scoparia dulcis, Medicinal plant, Shoot proliferation, Micropropagation, Acclimatization, IAA (indoleacetic acid), IBA(indolebutanoic acid), NAA(α-naphthaleneacetic acid), BAP(benzylamino purine) DOI: 10.3329/bjsir.v44i3.4408 Bangladesh J. Sci. Ind. Res. 44(3), 341-346, 2009

scholarly journals In Vitro Mass Propagation of Mimosa pudica L., Using Shoot Tip and Nodal Explants

1970 ◽  
Vol 45 (2) ◽  
pp. 95-100 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
Rebeka Sultana ◽  
Miskat Ara Akhter Jahan ◽  
Rahima Khatun
Keyword(s):  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Shoot Tip ◽  
Nodal Explants ◽  
Mimosa Pudica ◽  
Mass Propagation ◽  
Half Strength ◽  
Proliferation In Vitro

An efficient protocol was established for in vitro mass propagation of a valuable medicinal shrubby plant, Mimosa pudica Linn., from shoot tip and nodal explants. Optimum in vitro shoot induction was observed from nodal explants on MS basal medium supplemented with 1.5 mg/l BAP + 0.5 mg/l NAA, in which 88.2% of the explants produced 9 shoots per culture within 3-4 weeks. Repeated subcultures in the same medium, resulted rapid shoot multiplication with 20.4 ± 1.20 shoots per culture within 12 weeks. The healthy in vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IBA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 80%. Key words: Mimosa pudica; Medicinal plant; Shoot proliferation; In vitro mass propagation; Acclimatization DOI: 10.3329/bjsir.v45i2.5704Bangladesh J. Sci. Ind. Res. 45(2), 95-100, 2010

scholarly journals Callus Induction and High Frequency Regeneration of Plantlets of Scoparia dulcis L., a Perennial Medicinal Herb, Through Auxiliary Shoot Proliferation

1970 ◽  
Vol 18 (1) ◽  
pp. 75-83 ◽  
Author(s):  
A.K.M. Sayeed Hassan ◽  
Farhana Afroz ◽  
Laila Shamroze Bari ◽  
John Liton Munshi ◽  
Miskat Ara Akter Jahan ◽  
...  
Keyword(s):  
Leaf Shape ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Medicinal Herb ◽  
Nodal Segments ◽  
Scoparia Dulcis ◽  
Regenerated Plants ◽  
Nodular Callus ◽  
Half Strength

Green compact nodular callus was observed within three weeks from nodal segments of a perennial medicinal herb Scoparia dulcis L. on MS basal medium supplemented with 1.5 mg/l BAP + 0.2 mg/I NAA. The callus produced large number of shoots when subcultured on MS with 0.5 mg/l BAP + 0.1 mg/l NAA. In vitro raised shoots rooted on half strength of MS with 1.0 mg/l IBA + 1.0 mg/l NAA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for seven days before transplanting in pots where plantlets were reared for three weeks. The survival rate of plantlets was found to be 85%. Regenerated plants were morphologically uniform having normal leaf shape and growth. Key words: Scoparia dulcis, Medicinal plant, Shoot proliferation, Acclimatization D.O.I. 10.3329/ptcb.v18i1.3268 Plant Tissue Cult. & Biotech. 18(1): 75-83, 2008 (June)

scholarly journals In vitro plant regeneration of Coccinea cordifolia (Linn.) Cogn., an anti-diabetic medicinal plant

2012 ◽  
Vol 47 (2) ◽  
pp. 187-190
Author(s):  
Chapol Kumar Roy ◽  
John Liton Munshia ◽  
Nadira Begum ◽  
Rahima Khatun ◽  
AKM Sayeed Hassanb
Keyword(s):  
Plant Regeneration ◽  
Medicinal Plant ◽  
Basal Medium ◽  
Nodal Explants ◽  
Direct Organogenesis ◽  
In Vitro Plant Regeneration ◽  
Half Strength ◽  
In Vitro Plant ◽  
Regenerated Plantlets

An efficient protocol was developed for in vitro plant regeneration of a popularly used anti-diabetic medicinal plant, Coccinea cordifolia (Linn.) Cogn.(Cucurbitaceae) through direct organogenesis using shoot tip and nodal explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP, in which 88.2% of nodal explants responded to produce maximum number (6.2 ± 0.58) of shoots per culture. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IBA. The survival rate of regenerated plantlets was 85%.   DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11450   Bangladesh J. Sci. Ind. Res. 47(2), 187-190, 2012  

scholarly journals In vitro shoot proliferation and plant regeneration of Plumbago indica L. (Ractochita), a rare medicinal shrub of Bangladesh

2012 ◽  
Vol 47 (2) ◽  
pp. 197-202
Author(s):  
Fowzia Haque ◽  
AKM Sayeed Hassan ◽  
Miskat Ara Akhter Jahan ◽  
Shyamal K Roy
Keyword(s):  
Plant Regeneration ◽  
Room Temperature ◽  
Shoot Proliferation ◽  
Nodal Explants ◽  
Ms Medium ◽  
Plumbago Indica ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
In Vitro Shoot Proliferation

A protocol was established for in vitro shoot proliferation and plant regeneration of a rare medicinal shrub of Bangladesh, Plumbago indica L. (Plumbaginaceae) using shoot tip and nodal explants. Best shoot induction was observed on MS medium supplemented with 0.5 mg/l       BAP, in which 92% of nodal explants responded to produce maximum number (42.8 ± 1.18) of shoots per explants. In vitro raised shoots       rooted on half strength of MS medium with 0.5 mg/l IAA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting them in pots containing soil where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 82%.   DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11452   Bangladesh J. Sci. Ind. Res. 47(2), 197-202, 2012    

scholarly journals Indirect in vitro Regeneration of Viola canescens Wall. ex, Roxb. by using Leaf Calli

2018 ◽  
Vol 28 (2) ◽  
pp. 215-222 ◽  
Author(s):  
Arun Kumar Khajuria ◽  
NS Bisht
Keyword(s):  
Plant Regeneration ◽  
Natural Population ◽  
Plant Tissue ◽  
In Vitro Regeneration ◽  
Soil Condition ◽  
Medicinal Herb ◽  
Growth Chamber ◽  
Half Strength ◽  
Number Of Shoots

An efficient indirect plant regeneration protocol was developed for Viola canescens, an important medicinal herb used in broad spectra of diseases in number of folk medicines since aeon. Excessive use of this plant without any rehabilitating measure has led to decline its natural population. Present investigation reports the use of zeatin to regenerate the plant from the callus on MS following its acclimatization on the soil condition. Calli of the plant responded positively to zeatin and maximum number of shoots 13.07 ± 2.01 were obtained when 9.12 μM concentration of zeatin was used. Regenerated shoots were subsequently rooted with IBA on MS and half strength MS and showed maximum number of roots 14.13 ± 1.64 after 60 days when medium was fortified with 4.92 μM IBA, followed by transferring them to soil condition, acclimatization of the plantlet was carried in growth chamber and then finally to the field for their survival where it showed 80% survival. Plant Tissue Cult. & Biotech. 28(2): 215-222, 2018 (December)

scholarly journals In vitro Regeneration of Grass Pea (Lathyrus sativus L.)

2016 ◽  
Vol 4 (2) ◽  
pp. 1-8 ◽  
Author(s):  
P Saha ◽  
M Afrin ◽  
AKM Mohiuddin ◽  
AM Shohael
Keyword(s):  
In Vitro Regeneration ◽  
Basal Medium ◽  
Multiple Shoots ◽  
Nodal Explants ◽  
Lathyrus Sativus ◽  
Naphthalene Acetic Acid ◽  
Grass Pea ◽  
Half Strength ◽  
Lathyrus Sativus L

In vitro regeneration protocol for grass pea (Lathyrus sativus L.) was optimized using different concentrations and combinations of growth regulators. Direct shoot regeneration obtained through shoot organogenesis from different explants of grass pea cultured on MS medium supplemented with Gamborg B5 vitamin containing 6-benzylaminopurine (BAP), Thidiazuron (TDZ) and ?-naphthalene acetic acid (NAA). Highest percentage of shoots were obtained at 4.0 mg/l of BAP on nodal explants. Stunted multiple shoots were developed from nodal explants while 1.5 mg/l TDZ was used. About 56% of direct shoots were also obtained, while the combination of BAP (4.0 mg/l) and NAA (0.5 mg/l) were used. Regenerated plantlets were rooted most effectively (40%) in rooting medium containing half strength of MS basal medium containing 1.0 mg/l NAA. Well rooted plantlets were further successfully acclimatized to ambient humidity level and grown in controlled environment until hardening.Jahangirnagar University J. Biol. Sci. 4(2): 1-8, 2015 (December)

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