scholarly journals Antimicrobial, Cytotoxic, Thrombolytic and Antioxidant Activities of Syzygium fruticosum (Roxb.) DC.

2015 ◽  
Vol 17 (1) ◽  
pp. 51-54
Author(s):  
Somaia Haque Chadni ◽  
Abdullah Al Hasan ◽  
ATM Zafrul Azam
Keyword(s):  
Free Radical ◽  
Carbon Tetrachloride ◽  
Soluble Fraction ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Zone Of Inhibition ◽  
Aqueous Fraction

The crude methanol extracts of the bark and leaves of Syzygium fruticosum (Roxb.) DC. were partitioned with petroleum ether, carbon tetrachloride, chloroform and water for biological investigation. In the brine shrimp lethality bioassay, carbon tetrachloride soluble fraction of leaf extract (LCTF) showed significant lethality having the LC50 value 0.65 ?g/ml. In free radical scavenging activity screening (DPPH assay), chloroform fraction of methanolic extract of bark of S. fruticosum showed the highest free radical scavenging activity with IC50 value 20.01 ?g/ml. In the microbiological investigation, only chloroform soluble fraction of bark (BCF) and aqueous fraction of bark (BAF) showed mild antimicrobial activity with zone of inhibition ranging from 7 to 14 mm as compared to standard ciprofloxacin (zone of inhibition of 50 mm). In the study for thrombolytic property, different extracts of S. fruticosum revealed varying degrees of thrombolytic activity ranging from 33.46% to 62.51%. DOI: http://dx.doi.org/10.3329/bpj.v17i1.22315 Bangladesh Pharmaceutical Journal 17(1): 51-54, 2014

scholarly journals Lens Aldose Reductase Inhibitory and Free Radical Scavenging Activity of Fractions of Chromolaena odorata (Siam Weed): Potential for Cataract Remediation

2016 ◽  
Vol 8 (3) ◽  
pp. 263-271
Author(s):  
Emmanuel O. AJANI ◽  
Saheed SABIU ◽  
Fisayo A. BAMISYAE ◽  
Nurain O. ISMAILA ◽  
Olayinka S. ABDULSALAM
Keyword(s):  
Free Radical ◽  
Aldose Reductase ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Chromolaena Odorata ◽  
Aqueous Fraction ◽  
Phenolic Constituents

Searching for effective and safe aldose reductase (AR) inhibitor agent is a major thrust area in the mainstream of anti-cataractogenic research. This study was set up to investigate the in vitro aldose reductase inhibitory (ARI) activity of fractions of methanolic extract of Chromolaena odorata leaves, on partially purified AR from goat lens, for potential use in the development of anticataractogenic agent. The phyto-constituents of the leaves were screened in aqueous and methanolic extracts and the free radical scavenging activities of the fractions were evaluated. The kinetics of the enzyme in the presence of fractions of the leaves was then compared. Phenol, flavonoid, alkaloid, saponin, terpenoid, quinones and phlobatannins were detected in both extracts. All the fractions inhibited AR in an uncompetitive manner, showing a reduced V max and Km when compared with glyceraldehyde. ARI activity was found to be the highest with aqueous fraction (IC50, 0.22 ± 0.01 mg/ml). All other fractions showed mild to moderate AR inhibition capacity, while it was found to be the lowest within hexane fraction (IC50, 1.20 ± 0.10 mg/ml). All the fractions showed free radical scavenging activity and metal chelating activity. The study confirmed the ARI and antioxidant capacity of Chromolaena odorata which may be due to its phenolic constituents, indicating that the plant may serve as a base for the development of anticataract agent. 

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