scholarly journals In vitro Regeneration of an Endangered Medicinal Plant Withania somnifera using Four Different Explants

2013 ◽  
Vol 23 (1) ◽  
Author(s):  
A. Arumugam ◽  
K. Gopinath
Keyword(s):  
Medicinal Plant ◽  
Withania Somnifera ◽  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Leaf Explant ◽  
Cow Dung ◽  
Endangered Medicinal Plant ◽  
Rooting Medium ◽  
Regenerated Plantlets

An efficient protocol was developed for highly regenerative capacity from leaf explant of Withania somnifera (L.) Dunal ? an endangered medicinal plant. Calli were regenerated from four different explants like leaves, cotyledons, hypocotyls and epicotyls. MS supplemented with different concentrations of 2,4?D, BAP and NAA were used. The calli (94.33 ± 1.20%) were obtained from the leaf explant in 2,4?D 3.0 mg/l. The highest number of multiple shoots (85.67 ± 0.88%) were obtained from the leaf callus at 4.0 mg/l BAP. Shootlets forming calli were transferred to the rooting medium containing 10.0 mg/l NAA to produce multiple roots (89.33 ± 0.88%). The regenerated rooted shootlets were transferred to small polythene bags, which contain a sterilized cow?dung, sand and red soil (1 : 2 : 3) and kept in a mist house. After acclimation in the mist house the regenerated plantlets were hardened in the greenhouse and transferred to soil, which showed 85% survival rate. This new protocol was standardized for easy mass propagation of W. somnifera using leaf explant.Plant Tissue Cult. & Biotech. 23(1): 79?85, 2013 (June)DOI: http://dx.doi.org/10.3329/ptcb.v23i1.15564

scholarly journals In vitro Regeneration of Wedelia calendulacea Less. - A Valuable Endangered Medicinal Plant

2020 ◽  
Vol 30 (2) ◽  
pp. 277-283
Author(s):  
Sabina Yesmin
Keyword(s):  
Root Formation ◽  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Shoot Tips ◽  
Nodal Explants ◽  
Nodal Segments ◽  
Best Response ◽  
Endangered Medicinal Plant ◽  
Regenerated Plantlets

Direct plant regeneration from shoot tips and nodal explants of Wedelia calendulacea Less. was carried out in MS with different concentrations and combinations of BAP, Kn and NAA. Of the two explants nodal segments showed the best response towards the formation of multiple shoots in MS containing BAP (2.0 mg/l), Kn (1.0 mg/l) and NAA (0.5 mg/l). In this combination, the maximum number of shoots/explant obtained was 18.33 ± 0.72 from shoot tips and 26.64 ± 0.47 from nodal explants. Cent per cent root formation was obtained in MS with IBA (0.2 mg/l) within three weeks. The in vitro regenerated plantlets were successfully transplanted to soil for further growth and development. Plant Tissue Cult. & Biotech. 30(2): 277-283, 2020 (December)

scholarly journals Micropropagation of Vanasushava pedata - An Endangered Medicinal Plant of South India

1970 ◽  
Vol 16 (2) ◽  
pp. 85-94 ◽  
Author(s):  
S Karuppusamy ◽  
C Kiranmai ◽  
V Aruna ◽  
T Pullaiah
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Continuous Production ◽  
Natural Habitat ◽  
Nodal Segments ◽  
Garden Soil ◽  
Axillary Shoot ◽  
Similar Frequency ◽  
Endangered Medicinal Plant

An efficient in vitro propagation of an endangered medicinal plant Vanasushava pedata (Apiaceae) by axillary shoot proliferation from nodal segments of mature plants was designed. The medium type and growth regulators markedly influenced in vitro regeneration of V. pedata. An in vitro plantlet production system has been investigated on MS with the synergistic combination of BA (5.0 mg/l), IAA (0.1 mg/l) and 3 % sucrose which promoted the maximum number of shoots (8.6) as well as enhanced shoot lengths. Subculturing of nodal segments from in vitro derived shoots on a similar medium enabled continuous production of healthy shoots with a similar frequency. Rooting was highest (100%) on half strength MS containing IAA (2.0 mg/l). Micropropagated plants established in garden soil and forest humus (1 : 1) were uniform and identical to the donor plants with respect of growth characteristics as well as floral features. These in vitro-raised plants grew normally in greenhouse and natural habitat without showing any morphological variation.  Key words: Vanasushava pedata, Medicinal plant, Nodal explants, Micropropagation, Successful acclimationDOI = 10.3329/ptcb.v16i2.1109Plant Tissue Cult. & Biotech. 16(2): 85-94, 2006 (December)

Micropropagation and assessment of genetic stability in Celastrus paniculatus: An endangered medicinal plant

Biologia ◽  
2013 ◽  
Vol 68 (4) ◽  
Author(s):  
Sunil Senapati ◽  
Subhashree Aparajita ◽  
Gyana Rout
Keyword(s):  
Medicinal Plant ◽  
Genetic Stability ◽  
In Vitro Regeneration ◽  
Random Amplified Polymorphic Dna ◽  
Basal Medium ◽  
Naphthaleneacetic Acid ◽  
Multiplication Rate ◽  
Endangered Medicinal Plant ◽  
Celastrus Paniculatus

AbstractA highly efficient protocol for in vitro regeneration of an indigenous, endangered medicinal plant Celastrus paniculatus was achieved using nodal explants. Murashige and Skoog (MS) basal medium supplemented with 0.5 mg/L 6-benzylaminopurine (BAP) and 0.1 mg/L naphthaleneacetic acid (NAA) showed maximum percentage of shoot multiplication (83.4%) with 8.2 shoots/explants. Maximum rooting of 73.3% with 4.8 roots/shoot was achieved on half-strength MS media supplemented with 0.5 mg/L indole-3-acetic acid (IAA) and the percentage of survival was 91% after acclimatization. Random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) marker study confirmed genetic stability for in vitro raised explants by showing 100% monomorphism. High multiplication rate associated with genetic stability ensure the efficacy of the present in vitro clonal propagation protocol of this important medicinal plant species.

scholarly journals Micropropagation of Salvia broussonetii Benth. - A Medicinal Plant Species

1970 ◽  
Vol 16 (1) ◽  
pp. 19-23 ◽  
Author(s):  
S Mederos-Molina
Keyword(s):  
Ascorbic Acid ◽  
Medicinal Plant ◽  
Plant Species ◽  
Culture Medium ◽  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Axillary Shoots ◽  
Half Strength ◽  
Important Medicinal Plant

The purpose of this study was to establish culture medium requirements for micropropagation of Salvia broussonetii Benth., an important medicinal plant. Cultures were initiated from axillary shoots collected from mature plants. Most satisfactory results were achieved using a MS.2 medium supplemented with 1 mM ascorbic acid, 1.44 µM GA3 and 1.11 µM BAP. Axillary nodes were used for in vitro regeneration of multiple shoots and best results were achieved with MS.2 medium plus 1.44 µM GA3, 2.66 µM BAP and 1.14 µM IAA. Shoots rooted without symptoms of chlorosis or necrosis in half-strength MS.2 medium plus 1.44 µM GA3 and 2.28 µM IAA.Key words: Axillary shoots, Micropropagation, Medicinal plant, Salvia broussonetiiDOI = 10.3329/ptcb.v16i1.1101Plant Tissue Cult. & Biotech. 16(1): 19-23, 2006 (June)

In vitro regeneration of an endangered medicinal plant Picrorhiza scrophulariiflora

2011 ◽  
Vol 55 (1) ◽  
pp. 169-172 ◽  
Author(s):  
P. Bantawa ◽  
O. Saha-Roy ◽  
S. Ghosh ◽  
T. Mondal
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Endangered Medicinal Plant ◽  
Picrorhiza Scrophulariiflora

scholarly journals Efficient in vitro Regeneration of a Medicinal Plant Harsinghar (Nyctanthes arbor-tristis L.)

2013 ◽  
Vol 22 (2) ◽  
pp. 137-142 ◽  
Author(s):  
S Bansal ◽  
AJ Bharati ◽  
YK Bansal
Keyword(s):  
Medicinal Plant ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Axillary Bud ◽  
Root Initiation ◽  
Maximum Frequency ◽  
Half Strength

In vitro propagation of Nyctanthes arbor-tristis L. has been successfully established from axillary bud explants on MS. Maximum number of multiple shoots was obtained on MS containing BAP (22.2 ?M). Half strength of MS (2% sucrose) supplemented with NAA (10.74 ?M) provided the maximum frequency of root initiation. The plantlets were successfully hardened. DOI: http://dx.doi.org/10.3329/ptcb.v22i2.14202 Plant Tissue Cult. & Biotech. 22(2): 137-142, 2012 (December)

scholarly journals In vitro Plant Regeneration from Mature Seed Explants of Withania somnifera (L.) Dunal, an Important, Rare and Endangered Medicinal Plant

2019 ◽  
Vol 11 (4) ◽  
pp. 387-391
Author(s):  
Sape Subba TATA ◽  
Geddam JYOTHIRMAYEE ◽  
Owk Aniel KUMAR
Keyword(s):  
Medicinal Plant ◽  
Withania Somnifera ◽  
Multiple Shoots ◽  
Mature Seed ◽  
Direct Organogenesis ◽  
Ms Medium ◽  
Regeneration System ◽  
Time Saving ◽  
Mature Seeds

Withania somnifera (L.) Dunal a member of the Solanaceae family, is a traditional medicinal plant commonly known in India as Ashwagandha. It is used for different diseases such as hiccup, cough, rheumatism, tuberculosis, and exhibits excellent antitumor and anti-bacterial activities as well. Direct organogenesis of plants using mature seeds provides faster response and is also a time saving approach, thus the present study was conducted to investigate the optimal concentrations and combinations of plant growth regulators with MS medium for the establishment of an efficient regeneration system in W. somnifera using mature seed as an explant. Therefore, an efficient in vitro protocol for high frequency regeneration has been developed using mature seeds as explant. In the present study, the multiple shoots along with embryogenic callus induction was best seen in MS medium supplemented with BAP (1.5 mg/L) and IAA (0.5 mg/L). Furthermore, MS medium fortified with GA3 (0.3 mg/L) and IBA (3.0 mg/L) alone was suited for shoot elongation and rhizogenesis respectively. The rooted plantlets were hardened and successfully established in the soil. The establishment of a highly reproducible regeneration system would greatly influence the efforts of improvement of the hereby studied medicinal plant species through useful gene transfer technology.

scholarly journals High Efficiency In vitro Regeneration and Genetic Stability of Corallocarpus epigaeus - An Endangered Medicinal Plant

2020 ◽  
Vol 30 (2) ◽  
pp. 219-229
Author(s):  
Spoorthi Veera ◽  
Pavani Chirumamilla ◽  
Shasthree Taduri
Keyword(s):  
High Efficiency ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Multiple Shoots ◽  
Inter Simple Sequence Repeat ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Multiple Shoot Induction ◽  
Endangered Medicinal Plant

Indirect regeneration of plantlets from multiple shoot induction of Corallocarpus epigaeus was obtained from leaf and nodal explants on MS with different concentrations of BAP in combination with IAA/IBA or IBA alone. Among all the combinations, BAP and IBA exhibited maximum regeneration. High frequency of multiple shoots (89%) was obtained on BAP (2.0 mg/l) and IBA (1.5 mg/l) in nodal explants. Maximum mean shoot length of 6.8 ± 0.33 cm was obtained in nodal explants cultured on BAP (1.0 mg/l) + IBA (0.5 mg/l), followed by leaf explants with 6.7 ± 0.47 cm on BAP (3.0 mg/l) + IAA (2.5 mg/l). The highest frequency of rooting (88.3%) was obtained on NAA (1.0 mg/l) and IBA (2.0 mg/l) with 21.83 ± 0.57 mean number of roots. The well-rooted healthy plantlets were acclimatized with a survival rate of 80%. Inter simple sequence repeat (ISSR) analysis revealed the genetic similarity of in vitro raised plants with the mother plant. Plant Tissue Cult. & Biotech. 30(2): 219-229, 2020 (December)

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