In Vitro Regeneration of Endangered Medicinal Plant Heliotropium kotschyi (Ramram)

2016 ◽  
pp. 103-112
Author(s):  
Manal Ahmed Sadeq ◽  
Malabika Roy Pathak ◽  
Ahmed Ali Salih ◽  
Mohammed Abido ◽  
Asma Abahussain
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Endangered Medicinal Plant

scholarly journals Micropropagation of Vanasushava pedata - An Endangered Medicinal Plant of South India

1970 ◽  
Vol 16 (2) ◽  
pp. 85-94 ◽  
Author(s):  
S Karuppusamy ◽  
C Kiranmai ◽  
V Aruna ◽  
T Pullaiah
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Continuous Production ◽  
Natural Habitat ◽  
Nodal Segments ◽  
Garden Soil ◽  
Axillary Shoot ◽  
Similar Frequency ◽  
Endangered Medicinal Plant

An efficient in vitro propagation of an endangered medicinal plant Vanasushava pedata (Apiaceae) by axillary shoot proliferation from nodal segments of mature plants was designed. The medium type and growth regulators markedly influenced in vitro regeneration of V. pedata. An in vitro plantlet production system has been investigated on MS with the synergistic combination of BA (5.0 mg/l), IAA (0.1 mg/l) and 3 % sucrose which promoted the maximum number of shoots (8.6) as well as enhanced shoot lengths. Subculturing of nodal segments from in vitro derived shoots on a similar medium enabled continuous production of healthy shoots with a similar frequency. Rooting was highest (100%) on half strength MS containing IAA (2.0 mg/l). Micropropagated plants established in garden soil and forest humus (1 : 1) were uniform and identical to the donor plants with respect of growth characteristics as well as floral features. These in vitro-raised plants grew normally in greenhouse and natural habitat without showing any morphological variation.  Key words: Vanasushava pedata, Medicinal plant, Nodal explants, Micropropagation, Successful acclimationDOI = 10.3329/ptcb.v16i2.1109Plant Tissue Cult. & Biotech. 16(2): 85-94, 2006 (December)

Micropropagation and assessment of genetic stability in Celastrus paniculatus: An endangered medicinal plant

Biologia ◽  
2013 ◽  
Vol 68 (4) ◽  
Author(s):  
Sunil Senapati ◽  
Subhashree Aparajita ◽  
Gyana Rout
Keyword(s):  
Medicinal Plant ◽  
Genetic Stability ◽  
In Vitro Regeneration ◽  
Random Amplified Polymorphic Dna ◽  
Basal Medium ◽  
Naphthaleneacetic Acid ◽  
Multiplication Rate ◽  
Endangered Medicinal Plant ◽  
Celastrus Paniculatus

AbstractA highly efficient protocol for in vitro regeneration of an indigenous, endangered medicinal plant Celastrus paniculatus was achieved using nodal explants. Murashige and Skoog (MS) basal medium supplemented with 0.5 mg/L 6-benzylaminopurine (BAP) and 0.1 mg/L naphthaleneacetic acid (NAA) showed maximum percentage of shoot multiplication (83.4%) with 8.2 shoots/explants. Maximum rooting of 73.3% with 4.8 roots/shoot was achieved on half-strength MS media supplemented with 0.5 mg/L indole-3-acetic acid (IAA) and the percentage of survival was 91% after acclimatization. Random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) marker study confirmed genetic stability for in vitro raised explants by showing 100% monomorphism. High multiplication rate associated with genetic stability ensure the efficacy of the present in vitro clonal propagation protocol of this important medicinal plant species.

scholarly journals In vitro Regeneration of an Endangered Medicinal Plant Withania somnifera using Four Different Explants

2013 ◽  
Vol 23 (1) ◽  
Author(s):  
A. Arumugam ◽  
K. Gopinath
Keyword(s):  
Medicinal Plant ◽  
Withania Somnifera ◽  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Leaf Explant ◽  
Cow Dung ◽  
Endangered Medicinal Plant ◽  
Rooting Medium ◽  
Regenerated Plantlets

An efficient protocol was developed for highly regenerative capacity from leaf explant of Withania somnifera (L.) Dunal ? an endangered medicinal plant. Calli were regenerated from four different explants like leaves, cotyledons, hypocotyls and epicotyls. MS supplemented with different concentrations of 2,4?D, BAP and NAA were used. The calli (94.33 ± 1.20%) were obtained from the leaf explant in 2,4?D 3.0 mg/l. The highest number of multiple shoots (85.67 ± 0.88%) were obtained from the leaf callus at 4.0 mg/l BAP. Shootlets forming calli were transferred to the rooting medium containing 10.0 mg/l NAA to produce multiple roots (89.33 ± 0.88%). The regenerated rooted shootlets were transferred to small polythene bags, which contain a sterilized cow?dung, sand and red soil (1 : 2 : 3) and kept in a mist house. After acclimation in the mist house the regenerated plantlets were hardened in the greenhouse and transferred to soil, which showed 85% survival rate. This new protocol was standardized for easy mass propagation of W. somnifera using leaf explant.Plant Tissue Cult. & Biotech. 23(1): 79?85, 2013 (June)DOI: http://dx.doi.org/10.3329/ptcb.v23i1.15564

scholarly journals In vitro shoot multiplication and conservation of Caralluma bhupenderiana Sarkaria - an endangered medicinal plant from South India

2011 ◽  
Vol 10 (46) ◽  
pp. 9328-9336 ◽  
Author(s):  
Ugraiah A ◽  
Raja Sreelatha V ◽  
Krishna Reddy P V ◽  
Rajasekhar K ◽  
S S ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
South India ◽  
Shoot Multiplication ◽  
Endangered Medicinal Plant

scholarly journals Seaweed Extract Promotes High-Frequency in vitro Regeneration of Solanum surattense Burm.f:A Valuable Medicinal Plant

2017 ◽  
Vol 11 (4) ◽  
pp. 134-141
Author(s):  
Packiaraj Gurusarava ◽  
Sadasivam Vinoth ◽  
Ganesan Prem Kumar ◽  
Pandiselvi .
Keyword(s):  
Medicinal Plant ◽  
High Frequency ◽  
In Vitro Regeneration ◽  
Seaweed Extract ◽  
Solanum Surattense
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