Combinatorial Approach Through In Vitro Regeneration and Phytochemical Profiling of Ceropegia media (Huber) Ans.: A Potential Way Forward in the Conservation of an Endangered Medicinal Plant from the Western Ghats in India

2020 ◽  
Author(s):  
Meena Pandey ◽  
Bhushan B. Dholakia ◽  
Ramesha H. Jayaramaiah ◽  
Sachin A. Punekar ◽  
Ashok P. Giri
Keyword(s):  
Medicinal Plant ◽  
Western Ghats ◽  
In Vitro Regeneration ◽  
Combinatorial Approach ◽  
Endangered Medicinal Plant ◽  
The Western Ghats

scholarly journals In vitro Regeneration of the Medicinal Plant, Plumbago zeylanica L. with Reference to a Unique Population in Maruthamalai, the Western Ghats, India

1970 ◽  
Vol 18 (2) ◽  
pp. 173-179 ◽  
Author(s):  
T. Mallikadevi ◽  
P. Senthilkumar ◽  
S. Paulsamy
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Basal Medium ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Garden Soil ◽  
Plumbago Zeylanica ◽  
Adventitious Shoot Formation ◽  
The Western Ghats

The in vitro regeneration of Plubago zeylanica exhibited that the callus was initiated in the basal medium containing BAP, NAA, 2, 4-D, and IBA.  The high amount (90%) of organic calli was induced in the basal medium supplemented with 2, 4-D, alone at 2.0 mg/l. In the subculture the adventitious shoot formation was prominently higher (83%) in the basal medium containing BAP, and NAA at 3.5 and 0.3 mg/l, respectively. IAA (1.0 mg/l)effectively produced higher percen-tage (90) of roots and root growth. After sequential hardening, survivability rate was observed to be significantly higher (80%) in the hardening medium containing garden soil, sand and vermicompost in the ratio of 1 : 1 : 1 by volume under greenhouse condition.  Key words: Plumbago zeylanica, In vitro regeneration, Medicinal plant D.O.I. 10.3329/ptcb.v18i2.3648 Plant Tissue Cult. & Biotech. 18(2): 173-179, 2008 (December)

scholarly journals Micropropagation of Vanasushava pedata - An Endangered Medicinal Plant of South India

1970 ◽  
Vol 16 (2) ◽  
pp. 85-94 ◽  
Author(s):  
S Karuppusamy ◽  
C Kiranmai ◽  
V Aruna ◽  
T Pullaiah
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Continuous Production ◽  
Natural Habitat ◽  
Nodal Segments ◽  
Garden Soil ◽  
Axillary Shoot ◽  
Similar Frequency ◽  
Endangered Medicinal Plant

An efficient in vitro propagation of an endangered medicinal plant Vanasushava pedata (Apiaceae) by axillary shoot proliferation from nodal segments of mature plants was designed. The medium type and growth regulators markedly influenced in vitro regeneration of V. pedata. An in vitro plantlet production system has been investigated on MS with the synergistic combination of BA (5.0 mg/l), IAA (0.1 mg/l) and 3 % sucrose which promoted the maximum number of shoots (8.6) as well as enhanced shoot lengths. Subculturing of nodal segments from in vitro derived shoots on a similar medium enabled continuous production of healthy shoots with a similar frequency. Rooting was highest (100%) on half strength MS containing IAA (2.0 mg/l). Micropropagated plants established in garden soil and forest humus (1 : 1) were uniform and identical to the donor plants with respect of growth characteristics as well as floral features. These in vitro-raised plants grew normally in greenhouse and natural habitat without showing any morphological variation.  Key words: Vanasushava pedata, Medicinal plant, Nodal explants, Micropropagation, Successful acclimationDOI = 10.3329/ptcb.v16i2.1109Plant Tissue Cult. & Biotech. 16(2): 85-94, 2006 (December)

Micropropagation and assessment of genetic stability in Celastrus paniculatus: An endangered medicinal plant

Biologia ◽  
2013 ◽  
Vol 68 (4) ◽  
Author(s):  
Sunil Senapati ◽  
Subhashree Aparajita ◽  
Gyana Rout
Keyword(s):  
Medicinal Plant ◽  
Genetic Stability ◽  
In Vitro Regeneration ◽  
Random Amplified Polymorphic Dna ◽  
Basal Medium ◽  
Naphthaleneacetic Acid ◽  
Multiplication Rate ◽  
Endangered Medicinal Plant ◽  
Celastrus Paniculatus

AbstractA highly efficient protocol for in vitro regeneration of an indigenous, endangered medicinal plant Celastrus paniculatus was achieved using nodal explants. Murashige and Skoog (MS) basal medium supplemented with 0.5 mg/L 6-benzylaminopurine (BAP) and 0.1 mg/L naphthaleneacetic acid (NAA) showed maximum percentage of shoot multiplication (83.4%) with 8.2 shoots/explants. Maximum rooting of 73.3% with 4.8 roots/shoot was achieved on half-strength MS media supplemented with 0.5 mg/L indole-3-acetic acid (IAA) and the percentage of survival was 91% after acclimatization. Random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) marker study confirmed genetic stability for in vitro raised explants by showing 100% monomorphism. High multiplication rate associated with genetic stability ensure the efficacy of the present in vitro clonal propagation protocol of this important medicinal plant species.

scholarly journals In vitro regeneration approaches for restoration of Ceropegia mohanramii—an endemic and critically endangered asclepiad

2019 ◽  
Vol 17 (1) ◽  
Author(s):  
Avinash A. Adsul ◽  
Jaykumar J. Chavan ◽  
Nikhil B. Gaikwad ◽  
Rajaram V. Gurav ◽  
Ghansham B. Dixit ◽  
...  
Keyword(s):  
Butyric Acid ◽  
Western Ghats ◽  
Large Scale ◽  
In Vitro Regeneration ◽  
Critically Endangered ◽  
Ms Medium ◽  
Regeneration System ◽  
The Western Ghats ◽  
Flowering In Vitro

Abstract The study aimed to develop an efficient, rapid, and large-scale in vitro regeneration system for propagation, conservation, and restoration of an endemic and critically endangered herb, Ceropegia mohanramii. The cultures were established using nodal explants on Murashige and Skoog’s (MS) medium supplemented with 6-benzylaminopurine (BAP: 1.0 mg/l). Nodal buds cultured on MS medium supplemented with BAP (2.0 mg/l) along with indole-3-butyric acid (IBA, 0.5 mg/l) resulted with production of maximum number of shoots (17.1 ± 1.2) in hundred percent of the cultures. MS medium supplemented with BAP (2.0 mg/l) along with diverse concentrations of indole-3acetic acid (IAA) promoted the in vitro flowering. In vitro regenerated shoots were transferred to one-half MS medium fortified with singular supplementation of auxins, where IBA (1.5 mg/l) served optimal for production of maximum number of roots (5.7 ± 0.6). In vitro derived plantlets were hardened under controlled conditions in a glasshouse and subsequently transferred to soil. Over 1200 saplings were transplanted to eight different localities of the Western Ghats where over 76% survival is recorded after 1 year of transplantation.

scholarly journals An efficient in vitro regeneration of Ceropegia noorjahaniae: an endemic and critically endangered medicinal herb of the Western Ghats

2014 ◽  
Vol 20 (3) ◽  
pp. 405-410 ◽  
Author(s):  
J. J. Chavan ◽  
A. S. Nalawade ◽  
N. B. Gaikwad ◽  
R. V. Gurav ◽  
G. B. Dixit ◽  
...  
Keyword(s):  
Western Ghats ◽  
In Vitro Regeneration ◽  
Medicinal Herb ◽  
Critically Endangered ◽  
Endangered Medicinal Herb ◽  
The Western Ghats

In vitro regeneration of an endangered medicinal plant Picrorhiza scrophulariiflora

2011 ◽  
Vol 55 (1) ◽  
pp. 169-172 ◽  
Author(s):  
P. Bantawa ◽  
O. Saha-Roy ◽  
S. Ghosh ◽  
T. Mondal
Keyword(s):  
Medicinal Plant ◽  
In Vitro Regeneration ◽  
Endangered Medicinal Plant ◽  
Picrorhiza Scrophulariiflora

scholarly journals In vitro Regeneration of an Endangered Medicinal Plant Withania somnifera using Four Different Explants

2013 ◽  
Vol 23 (1) ◽  
Author(s):  
A. Arumugam ◽  
K. Gopinath
Keyword(s):  
Medicinal Plant ◽  
Withania Somnifera ◽  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Leaf Explant ◽  
Cow Dung ◽  
Endangered Medicinal Plant ◽  
Rooting Medium ◽  
Regenerated Plantlets

An efficient protocol was developed for highly regenerative capacity from leaf explant of Withania somnifera (L.) Dunal ? an endangered medicinal plant. Calli were regenerated from four different explants like leaves, cotyledons, hypocotyls and epicotyls. MS supplemented with different concentrations of 2,4?D, BAP and NAA were used. The calli (94.33 ± 1.20%) were obtained from the leaf explant in 2,4?D 3.0 mg/l. The highest number of multiple shoots (85.67 ± 0.88%) were obtained from the leaf callus at 4.0 mg/l BAP. Shootlets forming calli were transferred to the rooting medium containing 10.0 mg/l NAA to produce multiple roots (89.33 ± 0.88%). The regenerated rooted shootlets were transferred to small polythene bags, which contain a sterilized cow?dung, sand and red soil (1 : 2 : 3) and kept in a mist house. After acclimation in the mist house the regenerated plantlets were hardened in the greenhouse and transferred to soil, which showed 85% survival rate. This new protocol was standardized for easy mass propagation of W. somnifera using leaf explant.Plant Tissue Cult. & Biotech. 23(1): 79?85, 2013 (June)DOI: http://dx.doi.org/10.3329/ptcb.v23i1.15564

scholarly journals IN VITRO CALLUS INDUCTION OF SMILAX WIGHTII A.DC AN ENDEMIC MEDICINAL PLANT

2015 ◽  
Vol 2 (1) ◽  
pp. 97-101
Author(s):  
Nantha kumar R ◽  
Abdul kaffoor H ◽  
Arumugasamy K ◽  
Shalimol A ◽  
Asha devi V
Keyword(s):  
Medicinal Plant ◽  
Western Ghats ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
The Family ◽  
Half Strength ◽  
Endemic Medicinal Plant ◽  
The Western Ghats ◽  
Rooting Response

Smilax wightii A.DC is an endemic medicinal plant belongs to the family smilacaceae and distributed in Kodanadu, The Nilgiri Hills, The Western Ghats, Southern India. The callus was obtained at the concentration of 1.5+0.05 mg/I TDZ with NAA. Highest number of shoots was observed in 2.0+0.04 mg/l BAP + Kn and followed by 2.0+0.04mg/l. The multiplied shoots were harvested and used for rooting on half strength MS medium containing indole-3-butyric acid and naphthalene acetic acid within 45 days. The best rooting response was achieved on half-strength MS medium supplemented with 2.0 mg/l IBA. The well rooted plantlets were acclimatized and successfully transferred to natural condition, where 85% plantlets were survived

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