Clinical and Histological Analysis of Oral Mucocele

Benign notochordal cell tumors (BNCTs) are considered to be benign intraosseous lesions of notochord origin; however, recent spine studies have suggested the possibility that some chordomas arise from BNCTs. Here, the authors describe two cases demonstrating histological features of BNCT and concomitant chordoma involving the clivus, which, to the best of the authors’ knowledge, have not been previously documented at this anatomical site.An 18-year-old female presented with an incidentally discovered clival mass. Magnetic resonance imaging revealed a 2.8-cm nonenhancing lesion in the upper clivus that was T2 hyperintense and T1 hypointense. She underwent an uneventful endoscopic transsphenoidal resection. Histologically, the tumor demonstrated areas of classic chordoma and a distinct intraosseous BNCT component. The patient completed adjuvant radiation therapy. Follow-up showed no recurrence at 18 months.A 39-year-old male presented with an incidentally discovered 2.8-cm clival lesion. The nonenhancing mass was T2 hyperintense and T1 hypointense. Surgical removal of the lesion was performed through an endoscopic transsphenoidal approach. Histological analysis revealed areas of BNCT with typical features of chordoma. Follow-up did not demonstrate recurrence at 4 years.These cases document histologically concomitant BNCT and chordoma involving the clivus, suggesting that the BNCT component may be a precursor of chordoma.

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Histology of the vertebral artery–dural junction: relevance to posterolateral approaches to the skull base

Journal of Neurosurgery ◽

10.3171/2019.9.jns191394 ◽

2019 ◽

pp. 1-6

Author(s):

Robert C. Rennert ◽

Martin P. Powers ◽

Jeffrey A. Steinberg ◽

Takanori Fukushima ◽

John D. Day ◽

...

Keyword(s):

Connective Tissue ◽

Vertebral Artery ◽

Skull Base ◽

Histological Analysis ◽

Foramen Magnum ◽

Entry Site ◽

Microsurgical Technique ◽

Vessel Injury ◽

Far Lateral ◽

Clear Delineation

OBJECTIVEThe far-lateral and extreme-lateral infrajugular transcondylar–transtubercular exposure (ELITE) and extreme-lateral transcondylar transodontoid (ELTO) approaches provide access to lesions of the foramen magnum, inferolateral to mid-clivus, and ventral pons and medulla. A subset of pathologies in this region require manipulation of the vertebral artery (VA)–dural interface. Although a cuff of dura is commonly left on the VA to avoid vessel injury during these approaches, there are varying descriptions of the degree of VA-dural separation that is safely achievable. In this paper the authors provide a detailed histological analysis of the VA-dural junction to guide microsurgical technique for posterolateral skull base approaches.METHODSAn ELITE approach was performed on 6 preserved adult cadaveric specimens. The VA-dural entry site was resected, processed for histological analysis, and qualitatively assessed by a neuropathologist.RESULTSHistological analysis demonstrated a clear delineation between the intima and media of the VA in all specimens. No clear plane was identified between the connective tissue of the dura and the connective tissue of the VA adventitia.CONCLUSIONSThe VA forms a contiguous plane with the connective tissue of the dura at its dural entry site. When performing posterolateral skull base approaches requiring manipulation of the VA-dural interface, maintenance of a dural cuff on the VA is critical to minimize the risk of vascular injury.

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Histological analysis of ovary development in the cultured silver pom-fret, Pampus argenteus

Journal of Fishery Sciences of China ◽

10.3724/sp.j.1118.2013.00293 ◽

2013 ◽

Vol 20 (2) ◽

pp. 293-298 ◽

Cited By ~ 1

Author(s):

Peng SUN ◽

Fei YIN ◽

Zhaohong SHI ◽

Shiming PENG ◽

Jiangang WANG

Keyword(s):

Histological Analysis ◽

Ovary Development ◽

Pampus Argenteus

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Physical properties of different Pulp Capping Materials and Histological Analysis of their effect on Dogs’Dental Pulp Tissue Healing

Egyptian Dental Journal ◽

10.21608/edj.2018.77260 ◽

2018 ◽

Vol 64 (3) ◽

pp. 2657-2667

Author(s):

Mai Hamdy ◽

Dalia Fayyad ◽

Mohamed Eldaharawy ◽

Enas Hegazy

Keyword(s):

Physical Properties ◽

Histological Analysis ◽

Pulp Tissue ◽

Pulp Capping ◽

Tissue Healing

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Liver rheumatoid nodules imitating liver malignancy: a rare occurrence

BMJ Case Reports ◽

10.1136/bcr-2020-234366 ◽

2020 ◽

Vol 13 (12) ◽

pp. e234366

Author(s):

Jason Wee ◽

Salar Sobhi ◽

Bastiaan De Boer ◽

Dan Xu

Keyword(s):

Histological Analysis ◽

Granulomatous Inflammation ◽

Needle Aspiration ◽

Upper Respiratory Tract ◽

Positron Emission Tomography Scan ◽

Liver Malignancy ◽

Positron Emission ◽

Right Lobe ◽

Upper Respiratory ◽

Tomography Scan

We describe a case of a 61-year-old man with a background of rheumatoid arthritis who presented to the emergency department with a single-reported episode of haemoptysis on the background of an upper respiratory tract infection. A CT scan revealed an incidental 40 mm mass in upper right lobe of the liver abutting the diaphragmatic surface. A subsequent positron emission tomography scan confirmed the mass and raised the possibility of another lesion in the liver raising the suspicion of malignancy. The case was complicated by the inability to perform a fine needle aspiration biopsy due to the mass’ proximity to the diaphragm. After discussion with the patient, it was decided to resect the affected liver segment. Histological analysis of the mass revealed localised necrotising granulomatous inflammation suggestive of a rheumatoid nodule, which is seldom reported in the literature.

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Detailed histological analysis of a thrombectomy-resistant ischemic stroke thrombus: a case report

Thrombosis Journal ◽

10.1186/s12959-021-00262-1 ◽

2021 ◽

Vol 19 (1) ◽

Author(s):

Senna Staessens ◽

Olivier François ◽

Linda Desender ◽

Peter Vanacker ◽

Tom Dewaele ◽

...

Keyword(s):

Ischemic Stroke ◽

Von Willebrand Factor ◽

Histological Analysis ◽

Mechanical Thrombectomy ◽

Neutrophil Extracellular Traps ◽

Extracellular Dna ◽

Extracellular Traps ◽

First Pass ◽

Von Willebrand ◽

Willebrand Factor

Abstract Background Mechanical removal of a thrombus by thrombectomy can be quite challenging. For reasons that are not fully understood, some thrombi require multiple passes to achieve successful recanalization, whereas other thrombi are efficiently removed in a single pass. Since first pass success is associated with better clinical outcome, it is important to better understand the nature of thrombectomy resistant thrombi. The aim of this study was therefore to characterize the cellular and molecular composition of a thrombus that was very hard to retrieve via mechanical thrombectomy. Case presentation In a patient that was admitted with a right middle cerebral artery M1-occlusion, 11 attempts using various thrombectomy devices and techniques were required for removal of the thrombus. This peculiar case provided a rare opportunity to perform an in-depth histopathological study of a difficult to retrieve thrombus. Thrombus material was histologically analyzed using hematoxylin and eosin, Martius Scarlet Blue stain (red blood cells and fibrin), Feulgen stain (DNA), von Kossa stain (calcifications) and immunohistochemical analysis of von Willebrand factor, platelets, leukocytes and neutrophil extracellular traps. Histological analysis revealed abnormally high amounts of extracellular DNA, leukocytes, von Willebrand factor and calcifications. Extracellular DNA stained positive for markers of leukocytes and NETs, suggesting that a significant portion of DNA is derived from neutrophil extracellular traps. Conclusion In this unique case of a nearly thrombectomy-resistant stroke thrombus, our study showed an atypical composition compared to the common structural features found in ischemic stroke thrombi. The core of the retrieved thrombus consisted of extracellular DNA that colocalized with von Willebrand factor and microcalcifications. These results support the hypothesis that von Willebrand factor, neutrophil extracellular traps and microcalcifications contribute to mechanical thrombectomy resistance. Such information is important to identify novel targets in order to optimize technical treatment protocols and techniques to increase first pass success rates.

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