Evidences of Serological Studies for The Presence of Infectious Bovine Rhinotracheitis IBR, In Albania

Bovine herpesvirus 1 (BHV-1), the causative agent of infectious bovine rhinotracheitis (IBR), is considered to be the most common viral pathogen found in bovine. Virus enters through aerosol route or by direct contact with the nasal secretion in case of respiratory tract infection and by direct contact or by semen containing virus in case of genital infection. For the first time in Albania, this study was conducted to know the status of bovine herpesvirus-1 (BHV-1) antibodies in the bovines of the selected area of Albania. Antibody level was measured using a commercial indirect ELISA. A total of 263 collected serum samples from 7 areas of Albania were subjected to serum neutralization test for detection of BHV-1 antibodies by using of Indirect-ELISA kits. The chi-square test was used for comparison of results between regions and in this study p Values>0.01 was considered statistically no-significant at the 0.01 level. From these results we had an indication about the antibody prevalence of IBR infection respectively, 96% in Terpan-Berat, 52% in Fejza-Has, 50% in Kavajë, 33% in Rrëshen, 14.3% in Guras-Pogradec, 10% in Drenovë-Korçë and 0% in Fier. The prevalence was ranged from 10% to 96% among seropositive herds in this study. In conclusion, results of this study clearly established for the first time that BHV-1 is subclinical prevalent virus in bovine in Albania. Further studies are needed to prevent the spread of this viral infection in Albania.BHV-1,

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First Report on the Detection and Molecular Characterization of Bovine Herpesvirus 1 from a Clinical case of Infectious Bovine Rhinotracheitis in Pakistan

Pakistan Veterinary Journal ◽

10.29261/pakvetj/2020.084 ◽

2021 ◽

Vol 41 (01) ◽

pp. 160-162

Author(s):

Aayesha Riaz

Keyword(s):

Molecular Characterization ◽

Nested Pcr ◽

Bovine Herpesvirus ◽

Nasal Discharge ◽

Infectious Bovine Rhinotracheitis ◽

Bovine Herpesvirus 1 ◽

Nasal Swabs ◽

Herpesvirus 1 ◽

First Time

Bovine herpesvirus 1 (BHV-1) is a noteworthy reason for many Cattle/Buffalo diseases. Infectious bovine rhinotracheitis (IBR) is one of the diseases which are caused by the BHV-1. In the present study a cow which was suspected of IBR was examined. The animal was suffering from fever and respiratory distress along with rhinitis (red nose), in appetence, and dyspnea. The nasal mucosa and muzzle were distinctly inflamed, with nasal discharge. DNA from blood samples and nasal swabs were subjected to nested PCR using glycoprotein B gene (gB) Primers. The samples were found positive for BHV-1 gB gene. Sequencing and phylogenetic analysis revealed close similarities with other BHV-1 gB gene sequences. The accession numbers assigned to this pioneer sequences in GenBank are MT449510 (BHV-1-Pak 1) and MT449511 (BHV-1-Pak 2). In this study, we reported for the first time the detection of DNA of BHV-1 through nested PCR assay and molecular characterization of BHV-1 gB gene in Pakistan. This study will be useful in further diagnoses of BHV-1 in Pakistan and in development of BHV-1 vaccine to reduce economical losses due to IBR

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Characterization of envelope proteins of infectious bovine rhinotracheitis virus (bovine herpesvirus 1) by biochemical and immunological methods.

Journal of Virology ◽

10.1128/jvi.57.3.745-753.1986 ◽

1986 ◽

Vol 57 (3) ◽

pp. 745-753 ◽

Cited By ~ 60

Author(s):

R L Marshall ◽

L L Rodriguez ◽

G J Letchworth

Keyword(s):

Bovine Herpesvirus ◽

Envelope Proteins ◽

Immunological Methods ◽

Infectious Bovine Rhinotracheitis ◽

Bovine Herpesvirus 1 ◽

Herpesvirus 1 ◽

Infectious Bovine Rhinotracheitis Virus

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Antibodies against Bovine herpesvirus 1 in dairy herds in the state of Espirito Santo, Brasil

Revista CERES ◽

10.1590/s0034-737x2014000200017 ◽

2014 ◽

Vol 61 (2) ◽

pp. 280-283 ◽

Cited By ~ 2

Author(s):

Marcus Rebouças Santos ◽

Hanna Carolina Campos Ferreira ◽

Marcos Antônio dos Santos ◽

Giuliana Loreto Saraiva ◽

Natália Filardi Tafuri ◽

...

Keyword(s):

Dairy Cattle ◽

Neutralizing Antibodies ◽

Bovine Herpesvirus ◽

The State ◽

Serum Samples ◽

Bovine Herpesvirus 1 ◽

Espírito Santo ◽

Herpesvirus 1 ◽

Cattle Herds ◽

Espirito Santo

Bovine herpesvirus 1 (BoHV-1) causes major losses in worldwide livestock, affecting the respiratory and reproductive tracts of bovine. In the past decades, the number of cases in Brazil has been gradually increasing. Therefore, it is important to assess the distribution of infection in different regions of the country. In the state of Espírito Santo (ES) the BoHV 1 infection rate in dairy cattle herds is unknown. Thus, the aim of this study was to detect neutralizing antibodies against BoHV-1 in serum samples from 1,161 non-vaccinated cows from 59 dairy cattle herds in 23 municipalities of the Metropolitan, North, Northwest and South macro-regions. The identification of seropositive cows was evaluated by the virus neutralization test. The results showed that of all serum samples evaluated 775 (66.75%) had neutralizing antibodies against BoHV-1. Moreover, all herds were found positive; however, the percentage of positive cows varied among regions; 49.06%, 62.15%, 67.21% and 80.04% for the Metropolitan, South, North and Northwest macro-regions, respectively. In this study, the results clearly indicate the dissemination of the viral agent in dairy cattle in the ES state, requiring the monitoring and control of diseases related to BoHV-1 infection.

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4824667 Thymidine kinase deletion mutants of bovine herpesvirus-1, vaccines against infectious bovine rhinotracheitis containing same and methods for the production and use of same

Biotechnology Advances ◽

10.1016/0734-9750(89)90217-6 ◽

1989 ◽

Vol 7 (3) ◽

pp. 426

Keyword(s):

Thymidine Kinase ◽

Bovine Herpesvirus ◽

Deletion Mutants ◽

Infectious Bovine Rhinotracheitis ◽

Bovine Herpesvirus 1 ◽

Herpesvirus 1

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Detection of Bovine Herpesvirus-1-Specific IgM Using a Capture Enzyme Immune Assay with Isotype-Specific Monoclonal Antibodies

Journal of Veterinary Diagnostic Investigation ◽

10.1177/104063878900100209 ◽

1989 ◽

Vol 1 (2) ◽

pp. 139-145 ◽

Cited By ~ 5

Author(s):

Fernando Osorio ◽

Subramaniam Srikumaran ◽

Marvin Rhodes ◽

David Christensen ◽

Pushpa Srikumaran

Keyword(s):

Monoclonal Antibodies ◽

Solid Phase ◽

Bovine Herpesvirus ◽

Immunoglobulin M ◽

Viral Reactivation ◽

Clinical Samples ◽

Serum Samples ◽

Bovine Herpesvirus 1 ◽

Igm Antibody ◽

Herpesvirus 1

The detection of virus-specific immunoglobulin M (IgM) antibodies in acute-phase serum samples offers the possibility of making an accurate and rapid serologic diagnosis. We have developed a solid-phase capture assay that uses murine monoclonal antibodies specific for bovine IgM to separate the whole IgM fraction of a bovine serum sample. The IgM specific for bovine herpesvirus-1 (BHV-1) is then detected by the addition of viral antigen, which in turn is detected by BHV-1-specific monoclonal antibodies conjugated to horseradish peroxidase. A BHV-1 IgM antibody response was detected during the early postinfection period (7–40 days PI). Bovine herpesvirus-1 IgM antibody was not detected in sera taken from 3 animals following dexamethasone-induced viral reactivation. This method compares favorably with viral isolation, antigen detection in the clinical samples, and paired serology in the diagnosis of BHV-1 infection at a herd level.

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