Evaluation of the Efficacies of Rapid Antigen Test, Multiplex PCR, and Real-time PCR for the Detection of a Novel Influenza A (H1N1) Virus

2010 ◽  
Vol 30 (2) ◽  
pp. 147-152 ◽  
Author(s):  
Yusun Hwang ◽  
Kyounghee Kim ◽  
Miae Lee
Keyword(s):  
Multiplex Pcr ◽  
Real Time ◽  
Real Time Pcr ◽  
Influenza A ◽  
H1n1 Virus ◽  
Antigen Test ◽  
Influenza A H1n1 ◽  
Novel Influenza A

Detection of novel influenza A(H1N1) virus by real-time RT-PCR

2009 ◽  
Vol 45 (3) ◽  
pp. 203-204 ◽  
Author(s):  
David M. Whiley ◽  
Seweryn Bialasiewicz ◽  
Cheryl Bletchly ◽  
Cassandra E. Faux ◽  
Bruce Harrower ◽  
...  
Keyword(s):  
Real Time ◽  
Influenza A ◽  
H1n1 Virus ◽  
Rt Pcr ◽  
Influenza A H1n1 ◽  
Novel Influenza A

Development of a real-time RT-PCR assay for a novel influenza A (H1N1) virus

2010 ◽  
Vol 163 (2) ◽  
pp. 470-473 ◽  
Author(s):  
Tao Jiang ◽  
XiaoPing Kang ◽  
Yongqiang Deng ◽  
Hui Zhao ◽  
Xiaofeng Li ◽  
...  
Keyword(s):  
Real Time ◽  
Influenza A ◽  
H1n1 Virus ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Influenza A H1n1 ◽  
Novel Influenza A

scholarly journals Library of Prefabricated Locked Nucleic Acid Hydrolysis Probes Facilitates Rapid Development of Reverse-Transcription Quantitative Real-Time PCR Assays for Detection of Novel Influenza A/H1N1/09 Virus

2009 ◽  
Vol 55 (12) ◽  
pp. 2218-2222 ◽  
Author(s):  
Jürgen J Wenzel ◽  
Heiko Walch ◽  
Markus Bollwein ◽  
Hans Helmut Niller ◽  
Waltraud Ankenbauer ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Reverse Transcription ◽  
Real Time Pcr ◽  
Influenza A ◽  
Rapid Development ◽  
Locked Nucleic Acid ◽  
Quantitative Real Time Pcr ◽  
Influenza A H1n1 ◽  
Novel Influenza A

Abstract Background: The emergence of a novel pandemic human strain of influenza A (H1N1/09) has clearly demonstrated the need for flexible tools enabling the rapid development of new diagnostic methods. Methods: We designed a set of reverse-transcription quantitative real-time PCR (RT-qPCR) assays based on the Universal ProbeLibrary (UPL)—a collection of 165 presynthesized, fluorescence-labeled locked nucleic acid (LNA) hydrolysis probes—specifically to detect the novel influenza A virus. We evaluated candidate primer/UPL-probe pairs with 28 novel influenza A/H1N1/09 patient samples of European and Mexican origin. Results: Of 14 assays in the hemagglutinin (HA) and neuraminidase (NA) genes, 12 detected viral nucleic acids from diluted patient samples without need for further optimization. We characterized the diagnostic specificity of the 2 best-performing assays with a set of samples comprising various influenza virus strains of human and animal origin that showed no cross-reactivity. The diagnostic sensitivity of these 2 primer/probe combinations was in the range of 100–1000 genomic copies/mL. In comparison to a reference assay recommended by the German health authorities, the analytical sensitivities and specificities of the assays were equivalent. Conclusions: Facing the emergence of novel influenza A/H1N1/09, we were able to develop, within 2 days, a set of sensitive and specific RT-qPCR assays for the laboratory diagnosis of suspected cases. H1N1/09 served as a model to show the feasibility of the UPL approach for the expedited development of new diagnostic assays to detect emerging pathogens.

scholarly journals Polymeric LabChip Real-Time PCR as a Point-of-Care-Potential Diagnostic Tool for Rapid Detection of Influenza A/H1N1 Virus in Human Clinical Specimens

PLoS ONE ◽  
2012 ◽  
Vol 7 (12) ◽  
pp. e53325 ◽  
Author(s):  
Hyun-Ok Song ◽  
Je-Hyoung Kim ◽  
Ho-Sun Ryu ◽  
Dong-Hoon Lee ◽  
Sun-Jin Kim ◽  
...  
Keyword(s):  
Real Time ◽  
Diagnostic Tool ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Influenza A ◽  
H1n1 Virus ◽  
Point Of Care ◽  
Clinical Specimens ◽  
Influenza A H1n1

scholarly journals The mutation network for the hemagglutinin gene from the novel influenza A (H1N1) virus

2009 ◽  
Vol 54 (13) ◽  
pp. 2168-2170 ◽  
Author(s):  
YunGang He ◽  
GuoHui Ding ◽  
Chao Bian ◽  
Zhong Huang ◽  
Ke Lan ◽  
...  
Keyword(s):  
Influenza A ◽  
H1n1 Virus ◽  
The Novel ◽  
Influenza A H1n1 ◽  
Hemagglutinin Gene ◽  
Novel Influenza A
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