The Influences of Different Ratios of Biphasic Calcium Phosphate and Collagen Augmentation on Posterior Lumbar Spinal Fusion in Rat Model

Abstract Background: Despite widespread use of femoral-sourced allografts in clinical spinal fusion procedures and the increasing interest in using femoral reamer–irrigator–aspirator (RIA) autograft in clinical bone grafting, few studies have examined the efficacy of femoral grafts compared to iliac crest grafts in spinal fusion. The objective of this study was to directly compare the use of autologous iliac crest with syngeneic femoral and iliac allograft bone in the rat model of lumbar spinal fusion.Methods: Single-level bilateral posterolateral intertransverse process lumbar spinal fusion surgery was performed on Lewis rats divided into three experimental groups: iliac crest autograft; syngeneic iliac crest allograft; and syngeneic femoral allograft bone. Eight weeks postoperatively, fusion was evaluated via microCT analysis, manual palpation and histology. In vitro analysis of the colony-forming and osteogenic capacity of bone marrow cells derived from rat femurs and hips was also performed to determine whether there was a correlation with the fusion efficacy of these graft sources.Results: Although no differences were observed between groups in CT fusion mass volumes, iliac allografts displayed an increased number of radiographically fused fusion masses and a higher rate of bilateral fusion via manual palpation. Histologically, hip-derived grafts showed better integration with host bone than femur derived ones, likely associated with the higher concentration of osteogenic progenitor cells observed in hip-derived bone marrow. Conclusions: This study demonstrates the feasibility of using syngeneic allograft bone in place of autograft bone within inbred rat fusion models and highlights the need for further study of femoral-derived grafts in fusion.

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Ketogenic diet delays spinal fusion and decreases bone mass in posterolateral lumbar spinal fusion: an in vivo rat model

Acta Neurochirurgica ◽

10.1007/s00701-018-3616-7 ◽

2018 ◽

Vol 160 (10) ◽

pp. 1909-1916 ◽

Cited By ~ 1

Author(s):

Qi Liu ◽

Xiaomeng Wang ◽

Zucheng Huang ◽

Junhao Liu ◽

Jianyang Ding ◽

...

Keyword(s):

Bone Mass ◽

Spinal Fusion ◽

Ketogenic Diet ◽

Rat Model ◽

Lumbar Spinal Fusion ◽

Lumbar Spinal

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The effects of varenicline on lumbar spinal fusion in a rat model

The Spine Journal ◽

10.1016/j.spinee.2019.07.015 ◽

2020 ◽

Vol 20 (2) ◽

pp. 300-306

Author(s):

Jason R. Kang ◽

Juliane D Glaeser ◽

Brian Karamian ◽

Linda Kanim ◽

Zachary NaPier ◽

...

Keyword(s):

Spinal Fusion ◽

Rat Model ◽

Lumbar Spinal Fusion ◽

Lumbar Spinal

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P143. Effects of 5-Lipoxygenase Inhibition on Posterolateral Lumbar Spinal Fusion in the Rat Model

The Spine Journal ◽

10.1016/j.spinee.2008.06.785 ◽

2008 ◽

Vol 8 (5) ◽

pp. 169S-170S

Author(s):

Colin Harris ◽

Mitchell Reiter ◽

J. Patrick O'Connor ◽

Michael Vives ◽

Michael Loeven ◽

...

Keyword(s):

Spinal Fusion ◽

Rat Model ◽

Lumbar Spinal Fusion ◽

Lipoxygenase Inhibition ◽

Lumbar Spinal

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Comparing the efficacy of syngeneic iliac and femoral allografts with iliac crest autograft in a rat model of lumbar spinal fusion

Journal of Orthopaedic Surgery and Research ◽

10.1186/s13018-020-01936-8 ◽

2020 ◽

Vol 15 (1) ◽

Author(s):

Christina Holmes ◽

Benjamin D. Elder ◽

Wataru Ishida ◽

Alexander Perdomo-Pantoja ◽

John Locke ◽

...

Keyword(s):

Bone Marrow ◽

Spinal Fusion ◽

Rat Model ◽

Iliac Crest ◽

Bone Marrow Cells ◽

Allograft Bone ◽

Lumbar Spinal Fusion ◽

Manual Palpation ◽

Inbred Rat ◽

Lumbar Spinal

Abstract Background Despite widespread use of femoral-sourced allografts in clinical spinal fusion procedures and the increasing interest in using femoral reamer–irrigator–aspirator (RIA) autograft in clinical bone grafting, few studies have examined the efficacy of femoral grafts compared to iliac crest grafts in spinal fusion. The objective of this study was to directly compare the use of autologous iliac crest with syngeneic femoral and iliac allograft bone in the rat model of lumbar spinal fusion. Methods Single-level bilateral posterolateral intertransverse process lumbar spinal fusion surgery was performed on Lewis rats divided into three experimental groups: iliac crest autograft, syngeneic iliac crest allograft, and syngeneic femoral allograft bone. Eight weeks postoperatively, fusion was evaluated via microCT analysis, manual palpation, and histology. In vitro analysis of the colony-forming and osteogenic capacity of bone marrow cells derived from rat femurs and hips was also performed to determine whether there was a correlation with the fusion efficacy of these graft sources. Results Although no differences were observed between groups in CT fusion mass volumes, iliac allografts displayed an increased number of radiographically fused fusion masses and a higher rate of bilateral fusion via manual palpation. Histologically, hip-derived grafts showed better integration with host bone than femur derived ones, likely associated with the higher concentration of osteogenic progenitor cells observed in hip-derived bone marrow. Conclusions This study demonstrates the feasibility of using syngeneic allograft bone in place of autograft bone within inbred rat fusion models and highlights the need for further study of femoral-derived grafts in fusion.

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Comparing the Efficacy of Syngeneic Iliac and Femoral Allografts with Iliac Crest Autograft in a Rat Model of Lumbar Spinal Fusion

10.21203/rs.3.rs-27369/v1 ◽

2020 ◽

Author(s):

Christina Holmes ◽

Benjamin D. Elder ◽

Wataru Ishida ◽

Alexander Perdomo Pantoja ◽

John Locke ◽

...

Keyword(s):

Spinal Fusion ◽

Rat Model ◽

Iliac Crest ◽

Bone Marrow Cells ◽

Allograft Bone ◽

Lumbar Spinal Fusion ◽

Manual Palpation ◽

Inbred Rat ◽

Lumbar Spinal

Abstract Background: Despite widespread use of femoral-sourced allografts in clinical spinal fusion procedures and the increasing interest in using femoral reamer–irrigator–aspirator (RIA) autograft in clinical bone grafting, few studies have examined the efficacy of femoral grafts compared to iliac crest grafts in spinal fusion. The objective of this study was to directly compare the use of autologous iliac crest with syngeneic femoral and iliac allograft bone in the rat model of lumbar spinal fusion.Methods: Single-level bilateral posterolateral intertransverse process lumbar spinal fusion surgery was performed on Lewis rats divided into three experimental groups: iliac crest autograft; syngeneic iliac crest allograft; and syngeneic femoral allograft bone. Eight weeks postoperatively, fusion was evaluated via microCT analysis, manual palpation and histology. In vitro analysis of the colony-forming and osteogenic capacity of bone marrow cells derived from rat femurs and hips was also performed to determine whether there was a correlation with the fusion efficacy of these graft sources.Results: Although no differences were observed between groups in CT fusion rates or fusion mass volumes, iliac allografts displayed a higher rate of bilateral fusion via manual palpation. Histologically, hip-derived grafts showed better integration with host bone than femur derived ones, likely associated with the higher concentration of osteogenic progenitor cells observed in hip-derived bone marrow.Conclusions: This study demonstrates the feasibility of using syngeneic allograft bone in place of autograft bone within inbred rat fusion models and highlights the need for further study of femoral-derived grafts in fusion.

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Guideline update for the performance of fusion procedures for degenerative disease of the lumbar spine. Part 2: Assessment of functional outcome following lumbar fusion

Journal of Neurosurgery Spine ◽

10.3171/2014.4.spine14258 ◽

2014 ◽

Vol 21 (1) ◽

pp. 7-13 ◽

Cited By ~ 15

Author(s):

Zoher Ghogawala ◽

Daniel K. Resnick ◽

William C. Watters ◽

Praveen V. Mummaneni ◽

Andrew T. Dailey ◽

...

Keyword(s):

Lumbar Spine ◽

Functional Outcome ◽

Spinal Fusion ◽

Lumbar Fusion ◽

Minimum Clinically Important Difference ◽

Degenerative Disease ◽

Psychological State ◽

Lumbar Spinal Fusion ◽

Patient Reported ◽

Lumbar Spinal

Assessment of functional patient-reported outcome following lumbar spinal fusion continues to be essential for comparing the effectiveness of different treatments for patients presenting with degenerative disease of the lumbar spine. When assessing functional outcome in patients being treated with lumbar spinal fusion, a reliable, valid, and responsive outcomes instrument such as the Oswestry Disability Index should be used. The SF-36 and the SF-12 have emerged as dominant measures of general health-related quality of life. Research has established the minimum clinically important difference for major functional outcomes measures, and this should be considered when assessing clinical outcome. The results of recent studies suggest that a patient's pretreatment psychological state is a major independent variable that affects the ability to detect change in functional outcome.

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Are the Roland Morris Disability Questionnaire and Oswestry Disability Index interchangeable in patients after lumbar spinal fusion?

Journal of Back and Musculoskeletal Rehabilitation ◽

10.3233/bmr-200206 ◽

2021 ◽

pp. 1-7

Author(s):

R.F.M.R. Kersten ◽

J. Fikkers ◽

N. Wolterbeek ◽

F.C. Öner ◽

S.M. van Gaalen

Keyword(s):

Back Pain ◽

Spinal Fusion ◽

Outcome Measures ◽

Oswestry Disability Index ◽

Roland Morris Disability Questionnaire ◽

Leg Pain ◽

Lumbar Spinal Fusion ◽

Patient Reported ◽

Disability Questionnaire ◽

Lumbar Spinal

BACKGROUND: Low back pain is a common health problem for which there are several treatment options. For optimizing clinical decision making, evaluation of treatments and research purposes it is important that health care professionals are able to evaluate the functional status of patients. Patient reported outcome measures (PROMs) are widely accepted and recommended. The Roland Morris Disability Questionnaire (RMDQ) and the Oswestry Disability Index (ODI) are the two mainly used condition-specific patient reported outcomes. Concerns regarding the content and structural validity and also the different scoring systems of these outcome measures makes comparison of treatment results difficult. OBJECTIVE: Aim of this study was to determine if the RMDQ and ODI could be used exchangeable by assessing the correlation and comparing different measurement properties between the questionnaires. METHODS: Clinical data from patients who participated in a multicenter RCT with 2 year follow-up after lumbar spinal fusion were used. Outcome measures were the RMDQ, ODI, Short Form 36 – Health Survey (SF-36), leg pain and back pain measured on a 0–100 mm visual analogue scale (VAS). Cronbach’s alpha coefficients, Spearman correlation coefficients, multiple regression analysis and Bland-Altman plots were calculated. RESULTS: three hundred and seventy-six completed questionnaires filled out by 87 patients were used. The ODI and RMDQ had both a good level of internal consistency. There was a very strong correlation between the RMDQ and the ODI (r= 0.87; p< 0.001), and between the VAS and both the ODI and RMDQ. However, the Bland-Altman plot indicated bad agreement between the ODI and RMDQ. CONCLUSIONS: The RMDQ and ODI cannot be used interchangeably, nor is there a possibility of converting the score from one questionnaire to the other. However, leg pain and back pain seemed to be predictors for both the ODI and the RMDQ.

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