`Stevens' cranberry was genetically engineered to confer tolerance to the broad spectrum herbicide glufosinate. Initially, herbicide tolerance was verified by spraying greenhouse plants with the commercial formulation Liberty. Although one transformant showed significant tolerance, the tolerance level was below that required to kill goldenrod, a common weed of cranberry beds. This transformant was propagated and the plants established outdoors in a coldframe, yielding a growth form more typical of field-grown plants than that of greenhouse-grown plants. These plants, as well as untransformed cranberry and goldenrod plants, were sprayed with various levels of the herbicide. The transformed plants were not killed at glufosinate concentrations up to 1000 ppm, although delayed growth did occur. Some runner tip injury was observed at 500 ppm as well as widespread shoot tip death at higher levels. The above-ground parts of goldenrod plants were killed at 400 ppm with significant injury at 200 ppm. Untransformed cranberry plants were killed at 300 ppm and had extensive tip death even at 100 ppm. Transformed cranberry plants with confirmed “field” tolerance were re-established in the greenhouse and new vegetative growth was forced. When these plants were sprayed with glufosinate, significant shoot tip injury was observed at levels as low as 100 ppm. The degree of herbicide tolerance of transformed cranberry appears to be modulated by the growth environment, which may affect the expression of the inserted genes or the physiological sensitivity of the impacted tissues.
Kog1/Raptor mediates metabolic rewiring during nutrient limitation by controlling SNF1/AMPK activity