scholarly journals Genotoxicity assessment of antidiabetic formulation (ADPHF6) in human lymphocytes by single cell gel electrophoresis (comet assay) - an in vitro study

2015 ◽  
Vol 6 ◽  
Author(s):  
Shanmugasundaram Devanand ◽  
Perumal Kumar ◽  
Sasikumar Changam ◽  
Cherian Sanjay ◽  
Cherian Kotturathu
Keyword(s):  
Comet Assay ◽  
Single Cell ◽  
Gel Electrophoresis ◽  
Human Lymphocytes ◽  
In Vitro Study ◽  
Vitro Study ◽  
Single Cell Gel Electrophoresis

Evaluation of genotoxicity after application of Listerine® on human lymphocytes by micronucleus and single cell gel electrophoresis assays

2011 ◽  
Vol 28 (3) ◽  
pp. 271-275 ◽  
Author(s):  
Hasan Türkez ◽  
Başak Toğar ◽  
Taner Arabacı
Keyword(s):  
Single Cell ◽  
Gel Electrophoresis ◽  
Human Lymphocytes ◽  
Oral Care ◽  
Control Group ◽  
Limited Information ◽  
Single Cell Gel Electrophoresis ◽  
Test Models ◽  
Mouth Rinses

Listerine (LN) is one of the most commonly used mouth rinses worldwide although very limited information is available concerning its genotoxicity. In another view, the biological safety profile of oral care products is frequently assumed on the basis of simplistic test models. Therefore, the present study was undertaken to investigate the in vitro genotoxic potential of LN using micronucleus and single cell gel electrophoresis tests as genetic endpoints. Different concentrations of LN (0–100% of ml/culture, v/v) were applied to whole human blood cultures ( n = 5). The result of the present study showed that there were no statistically significant differences ( p > 0.05) between the control group and the groups treated with LN alone in both analysed endpoints. In conclusion, our result first demonstrated the absence of genotoxicity of LN on human lymphocytes.

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