Aim: To analyze biological characteristics, malignant potential and expression of protei.os associated with epithelial-mesenchymal transition in murine lung carcinoma cells transduced with interferon-beta (ifn-{J) gene in baculovirus vector. Materials and Meth ods; The study was performed on Lewis lung carcinoma (LL) ceUs transduced with ifil-P gene in recombinant bacuJovirus vector. Biological characteristics of the LL cells were studied with the use of standard cell culture methods, cytogenetic and immunocyto chemical assays. Results: Recombinant baculovirus-mediated transduction ofLLcells with ifn-P gene resulted i.o significant decrease of cell growth rate and density both io compJete and serum-free medium.Also, LL ceUs transduction with ifn-P gene significantly inhibited ceU migration in vitro.Transduction of LL cells by baculovirus vector with or without ifn-{J gene caused significant geno tox.ic effect in these ceUs. Furthermore, ifn-{J gene transfer to lung carcinoma cells resulted in significant increase of nuclear ex pression ofpl(p < 0.01), p21w.IJ'e (p < 0.001),cytoplasmlc expression ofE-cadherin (p < 0.005) and inhibition oftranscrlption factors of epithelial-mesenchymal transition (EMn Twist (p <0.005) and Slug (p < 0.00 I) expression.Condusions: Transduction with ij11-{J gene of LL cells in recombinant baculovirus resulted in acquirement of less malignant phenotype i11 vitro and suppressed expression of proteins associated with EMT.
Adaptation to acidic extracellular pH induces epithelial-mesenchymal transition as stable phenotype in Lewis lung carcinoma model
