Applicability of Recombinant Laccases From the White-Rot Fungus Obba rivulosa for Mediator-Promoted Oxidation of Biorefinery Lignin at Low pH

Utilization of lignin-rich side streams has been a focus of intensive studies recently. Combining biocatalytic methods with chemical treatments is a promising approach for sustainable modification of lignocellulosic waste streams. Laccases are catalysts in lignin biodegradation with proven applicability in industrial scale. Laccases directly oxidize lignin phenolic components, and their functional range can be expanded using low-molecular-weight compounds as mediators to include non-phenolic lignin structures. In this work, we studied in detail recombinant laccases from the selectively lignin-degrading white-rot fungus Obba rivulosa for their properties and evaluated their potential as industrial biocatalysts for the modification of wood lignin and lignin-like compounds. We screened and optimized various laccase mediator systems (LMSs) using lignin model compounds and applied the optimized reaction conditions to biorefinery-sourced technical lignin. In the presence of both N–OH-type and phenolic mediators, the O. rivulosa laccases were shown to selectively oxidize lignin in acidic reaction conditions, where a cosolvent is needed to enhance lignin solubility. In comparison to catalytic iron(III)–(2,2,6,6-tetramethylpiperidin-1-yl)oxyl (TEMPO) oxidation systems, the syringyl-type lignin units were preferred in mediated biocatalytic oxidation systems.

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Demethylation of the lignin model dimer 1-(3′,4′-dimethoxyphenyl)-2-(o-methoxyphenoxy)-propane-1,3-diol by the white-rot fungus Phlebia tremellosa

Canadian Journal of Botany ◽

10.1139/b92-060 ◽

1992 ◽

Vol 70 (3) ◽

pp. 453-460 ◽

Cited By ~ 8

Author(s):

Ian D. Reid

Keyword(s):

Lignin Peroxidase ◽

White Rot Fungi ◽

White Rot ◽

Metabolic Inhibitors ◽

White Rot Fungus ◽

Lignin Biodegradation ◽

Extracellular Medium ◽

Lignin Model Dimer ◽

Lignin Model ◽

Model Dimer

Phlebia tremellosa dealkylates nonphenolic β-O-4-linked lignin model dimers; the phenolic products are oxidized by laccase. There is no evidence of α,β-cleavage as catalyzed by lignin peroxidase in some other white rot fungi. Methanol is the product of demethylation of 1-(3′,4′-dimethoxyphenyl)-2-(o-methoxyphenoxy)-propane-1,3-diol, and the demethylation reaction can be assayed by the release of radioactive CH3OH from the 4′-O14CH3 labelled compound. In older cultures, the methanol is metabolized to CO2. Demethylation is stimulated by oxygen and blocked by several common metabolic inhibitors. Both the mycelium and the extracellular medium are required for demethylation. Key words: lignin biodégradation, laccase, demethylation, lignin peroxidase.

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Physical and enzymatic properties of a new manganese peroxidase from the white-rot fungus Trametes pubescens strain i8 for lignin biodegradation and textile-dyes biodecolorization

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2018.12.053 ◽

2019 ◽

Vol 125 ◽

pp. 514-525 ◽

Cited By ~ 14

Author(s):

Hatem Rekik ◽

Nadia Zaraî Jaouadi ◽

Khelifa Bouacem ◽

Bilal Zenati ◽

Sidali Kourdali ◽

...

Keyword(s):

Manganese Peroxidase ◽

Textile Dyes ◽

Enzymatic Properties ◽

White Rot ◽

White Rot Fungus ◽

Lignin Biodegradation

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An extracellular yellow laccase from white rot fungus Trametes sp. F1635 and its mediator systems for dye decolorization

Biochimie ◽

10.1016/j.biochi.2018.02.015 ◽

2018 ◽

Vol 148 ◽

pp. 46-54 ◽

Cited By ~ 23

Author(s):

Shou-Nan Wang ◽

Qing-Jun Chen ◽

Meng-Juan Zhu ◽

Fei-Yang Xue ◽

Wei-Cong Li ◽

...

Keyword(s):

Dye Decolorization ◽

White Rot ◽

White Rot Fungus ◽

Mediator Systems ◽

Yellow Laccase

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Enhancement of lignin degradation and laccase activity in Pleurotus ostreatus by cotton stalk extract

Canadian Journal of Microbiology ◽

10.1139/w98-054 ◽

1998 ◽

Vol 44 (7) ◽

pp. 676-680 ◽

Cited By ~ 17

Author(s):

Orly Ardon ◽

Zohar Kerem ◽

Yitzhak Hadar

Keyword(s):

Oxygen Consumption ◽

Pleurotus Ostreatus ◽

Lignin Degradation ◽

Laccase Activity ◽

White Rot ◽

White Rot Fungus ◽

Lignin Biodegradation ◽

Cotton Stalk ◽

Fermentation System ◽

Uv Visible

The white rot fungus Pleurotus ostreatus was grown in a chemically defined solid state fermentation system amended with cotton stalk extract (CSE).Treated cultures exhibited increased laccase activity as well as enhanced lignin mineralization. Mineralization of [14C]lignin initialized 4 days earlier in CSE-supplemented cultures than in control cultures. Total mineralization in the first 16 days was 15% in the CSE-treated cultures, compared with only 7% in the controls. Cotton stalk extract also contained compounds that serve as substrates for laccase purified from P. ostreatus as shown by oxygen consumption, as well as changes in the UVvisible spectrum.Key words: cotton, Pleurotusostreatus, white rot, laccase, lignin biodegradation.

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Transcriptomics analysis reveals the high biodegradation efficiency of white-rot fungus Phanerochaete sordida YK-624 on native lignin

10.21203/rs.2.20480/v1 ◽

2020 ◽

Author(s):

Jianqiao Wang ◽

Tomohiro Suzuki ◽

Hideo Dohra ◽

Toshio Mori ◽

Hirokazu Kawagishi ◽

...

Keyword(s):

Gene Expression ◽

Lignin Degradation ◽

Ligninolytic Enzymes ◽

Organic Matrix ◽

Tca Cycle ◽

Underlying Mechanism ◽

White Rot ◽

White Rot Fungus ◽

Lignin Biodegradation ◽

Phanerochaete Sordida

Abstract Background Lignocellulosic biomass is an organic matrix composed of cellulose, hemicellulose, and lignin. In nature, lignin degradation by basidiomycetes is the key step in lignocellulose decay. The white-rot fungus Phanerochaete sordida YK-624 (YK-624) has been extensively studied due to its high lignin degradation ability. In our previous study, it was demonstrated that YK-624 can secrete lignin peroxidase and manganese peroxidase for lignin degradation. However, the underlying mechanism for lignin degradation by YK-624 remains unknown.Results Here, we analyzed YK-624 gene expression following growth under ligninolytic and nonligninolytic conditions and compared the differentially expressed genes in YK-624 to those in the model white-rot fungus P. chrysosporium by next-generation sequencing. More ligninolytic enzymes and lignin-degrading auxiliary enzymes were upregulated in YK-624. This might explain the high degradation efficiency of YK-624. In addition, the genes involved in energy metabolism pathways, such as the TCA cycle, oxidative phosphorylation, lipid metabolism, carbon metabolism and glycolysis, were upregulated under ligninolytic conditions in YK-624.Conclusions In the present study, the first differential gene expression analysis of YK-624 under ligninolytic and nonligninolytic conditions was reported. The results obtained in this study indicated that YK-624 produces more energy- and lignin-degrading enzymes for more efficient lignin biodegradation.

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Lignin Biodegradation by Selective White Rot Fungus and Its Potential Use in Wood Biomass Conversion

ACS Symposium Series - Materials, Chemicals, and Energy from Forest Biomass ◽

10.1021/bk-2007-0954.ch026 ◽

2007 ◽

pp. 409-421 ◽

Cited By ~ 1

Author(s):

T. Watanabe ◽

Y. Ohashi ◽

T. Tanabe ◽

Y. Honda ◽

K. Messner

Keyword(s):

Biomass Conversion ◽

White Rot ◽

White Rot Fungus ◽

Lignin Biodegradation ◽

Wood Biomass ◽

Potential Use

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Intracellular ferrireductase involved in Mn(IV)-reducing enzyme system to supply Mn(II) for lignin biodegradation by white-rot fungus Phanerochaete sordida YK-624

Enzyme and Microbial Technology ◽

10.1016/s0141-0229(02)00009-1 ◽

2002 ◽

Vol 30 (4) ◽

pp. 467-473 ◽

Cited By ~ 2

Author(s):

Hirofumi Hirai ◽

Takanori Itoh ◽

Ryuichiro Kondo ◽

Kokki Sakai ◽

Tomoaki Nishida

Keyword(s):

Enzyme System ◽

White Rot ◽

White Rot Fungus ◽

Lignin Biodegradation ◽

Phanerochaete Sordida

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Nutritional regulation of synthetic lignin (DHP) degradation by the selective white-rot fungus Phlebia (Merulius) tremellosa: effects of glucose and other cosubstrates

Canadian Journal of Botany ◽

10.1139/b91-021 ◽

1991 ◽

Vol 69 (1) ◽

pp. 147-155 ◽

Cited By ~ 16

Author(s):

Ian D. Reid ◽

Alain M. Deschamps

Keyword(s):

Energy Source ◽

Lignin Degradation ◽

Tricarboxylic Acid Cycle ◽

Wood Decay ◽

White Rot ◽

White Rot Fungus ◽

Lignin Biodegradation ◽

Nutritional Regulation ◽

Carbohydrate Degradation ◽

Lignin Metabolism

Phlebia tremellosa is a white-rot fungus which selectively degrades lignin, i.e., its ratio of lignin degradation to carbohydrate degradation during wood decay is higher than that of "simultaneous" white rots. Its need for a cosubstrate to support lignin degradation, and the effect of glucose supply on rate and extent of lignin metabolism, were examined in a synthetic, nitrogen-limited medium. Lignin metabolism by P. tremellosa, like simultaneous white rots, requires a cosubstrate. Glucose partially represses lignin degradation, but it is metabolized to extracellular intermediates, including ethanol. Subsequent utilization of ethanol as energy source supports rapid lignin degradation. Phlebia tremellosa grows well with cellulose, glucose, xylose, ethanol, or lactate as sole carbon (energy) source, and more slowly with glycerol or methanol. It appears unable to use kraft lignin, ferulate, vanillin, or acetate as sole carbon source. Cellulose, glycerol, and ethanol efficiently supported degradation of ring-labelled lignin to CO2, whereas glucose, xylose, and lactate were less efficient cosubstrates; methanol did not support lignin degradation. A relationship between tricarboxylic acid cycle operation and metabolism of lignin ring carbons to CO2, is suggested. Key words: lignin biodegradation, cosubstrate, glucose, ethanol, selectivity.

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