Enhancement of lignin degradation and laccase activity in Pleurotus ostreatus by cotton stalk extract

1998 ◽  
Vol 44 (7) ◽  
pp. 676-680 ◽  
Author(s):  
Orly Ardon ◽  
Zohar Kerem ◽  
Yitzhak Hadar
Keyword(s):  
Oxygen Consumption ◽  
Pleurotus Ostreatus ◽  
Lignin Degradation ◽  
Laccase Activity ◽  
White Rot ◽  
White Rot Fungus ◽  
Lignin Biodegradation ◽  
Cotton Stalk ◽  
Fermentation System ◽  
Uv Visible

The white rot fungus Pleurotus ostreatus was grown in a chemically defined solid state fermentation system amended with cotton stalk extract (CSE).Treated cultures exhibited increased laccase activity as well as enhanced lignin mineralization. Mineralization of [14C]lignin initialized 4 days earlier in CSE-supplemented cultures than in control cultures. Total mineralization in the first 16 days was 15% in the CSE-treated cultures, compared with only 7% in the controls. Cotton stalk extract also contained compounds that serve as substrates for laccase purified from P. ostreatus as shown by oxygen consumption, as well as changes in the UV–visible spectrum.Key words: cotton, Pleurotusostreatus, white rot, laccase, lignin biodegradation.

scholarly journals Transcriptomics analysis reveals the high biodegradation efficiency of white-rot fungus Phanerochaete sordida YK-624 on native lignin

2020 ◽  
Author(s):  
Jianqiao Wang ◽  
Tomohiro Suzuki ◽  
Hideo Dohra ◽  
Toshio Mori ◽  
Hirokazu Kawagishi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lignin Degradation ◽  
Ligninolytic Enzymes ◽  
Organic Matrix ◽  
Tca Cycle ◽  
Underlying Mechanism ◽  
White Rot ◽  
White Rot Fungus ◽  
Lignin Biodegradation ◽  
Phanerochaete Sordida

Abstract Background Lignocellulosic biomass is an organic matrix composed of cellulose, hemicellulose, and lignin. In nature, lignin degradation by basidiomycetes is the key step in lignocellulose decay. The white-rot fungus Phanerochaete sordida YK-624 (YK-624) has been extensively studied due to its high lignin degradation ability. In our previous study, it was demonstrated that YK-624 can secrete lignin peroxidase and manganese peroxidase for lignin degradation. However, the underlying mechanism for lignin degradation by YK-624 remains unknown.Results Here, we analyzed YK-624 gene expression following growth under ligninolytic and nonligninolytic conditions and compared the differentially expressed genes in YK-624 to those in the model white-rot fungus P. chrysosporium by next-generation sequencing. More ligninolytic enzymes and lignin-degrading auxiliary enzymes were upregulated in YK-624. This might explain the high degradation efficiency of YK-624. In addition, the genes involved in energy metabolism pathways, such as the TCA cycle, oxidative phosphorylation, lipid metabolism, carbon metabolism and glycolysis, were upregulated under ligninolytic conditions in YK-624.Conclusions In the present study, the first differential gene expression analysis of YK-624 under ligninolytic and nonligninolytic conditions was reported. The results obtained in this study indicated that YK-624 produces more energy- and lignin-degrading enzymes for more efficient lignin biodegradation.

Induction of laccase activity in the white rot fungus Pleurotus ostreatus using water polluted with wheat straw extracts

2013 ◽  
Vol 133 ◽  
pp. 142-149 ◽  
Author(s):  
Alejandra Parenti ◽  
Elaia Muguerza ◽  
Amaia Redin Iroz ◽  
Alejandra Omarini ◽  
Enma Conde ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Pleurotus Ostreatus ◽  
Laccase Activity ◽  
White Rot ◽  
White Rot Fungus

Studies on Effect of NH4+-N on Indole Removal by Using the Laccase and the Laccase / ABTS Mediator System

2013 ◽  
Vol 726-731 ◽  
pp. 2274-2279
Author(s):  
Da Jun Ren ◽  
Xin Bao ◽  
Li Hua Liu ◽  
Qin Xu ◽  
Shu Qin Zhang ◽  
...  
Keyword(s):  
Pleurotus Ostreatus ◽  
Laccase Activity ◽  
Influence Factor ◽  
Sulphonic Acid ◽  
White Rot ◽  
White Rot Fungus ◽  
N Concentration ◽  
Mediator System ◽  
Positive Effect

NH4+-N was choiced as an influence factor for analysis the effect on the laccase activity from the white rot fungus Pleurotus ostreatus and indole degradation. The experiments were performed with different NH4+-N concentration, with a focus on the indole degradation by laccase in the presence of 2,2-Azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) or not. Results showed that NH4+-N had a positive effect on laccase activity and also enhanced the degradation of indole. Meanwhile, NH4+-N was oxidized to NO2-.

Nutritional regulation of synthetic lignin (DHP) degradation by the selective white-rot fungus Phlebia (Merulius) tremellosa: effects of glucose and other cosubstrates

1991 ◽  
Vol 69 (1) ◽  
pp. 147-155 ◽  
Author(s):  
Ian D. Reid ◽  
Alain M. Deschamps
Keyword(s):  
Energy Source ◽  
Lignin Degradation ◽  
Tricarboxylic Acid Cycle ◽  
Wood Decay ◽  
White Rot ◽  
White Rot Fungus ◽  
Lignin Biodegradation ◽  
Nutritional Regulation ◽  
Carbohydrate Degradation ◽  
Lignin Metabolism

Phlebia tremellosa is a white-rot fungus which selectively degrades lignin, i.e., its ratio of lignin degradation to carbohydrate degradation during wood decay is higher than that of "simultaneous" white rots. Its need for a cosubstrate to support lignin degradation, and the effect of glucose supply on rate and extent of lignin metabolism, were examined in a synthetic, nitrogen-limited medium. Lignin metabolism by P. tremellosa, like simultaneous white rots, requires a cosubstrate. Glucose partially represses lignin degradation, but it is metabolized to extracellular intermediates, including ethanol. Subsequent utilization of ethanol as energy source supports rapid lignin degradation. Phlebia tremellosa grows well with cellulose, glucose, xylose, ethanol, or lactate as sole carbon (energy) source, and more slowly with glycerol or methanol. It appears unable to use kraft lignin, ferulate, vanillin, or acetate as sole carbon source. Cellulose, glycerol, and ethanol efficiently supported degradation of ring-labelled lignin to CO2, whereas glucose, xylose, and lactate were less efficient cosubstrates; methanol did not support lignin degradation. A relationship between tricarboxylic acid cycle operation and metabolism of lignin ring carbons to CO2, is suggested. Key words: lignin biodegradation, cosubstrate, glucose, ethanol, selectivity.

scholarly journals Transcriptomics analysis and co-expression network revealing candidate genes for laccase activity of Lenzites gibbosa

2020 ◽  
Author(s):  
Jie Chen ◽  
Yujie Chi ◽  
Qingquan Zhao ◽  
Xin Hao
Keyword(s):  
Lignin Degradation ◽  
Laccase Activity ◽  
Expression Patterns ◽  
Enrichment Analysis ◽  
White Rot ◽  
White Rot Fungus ◽  
Mitochondrial Outer Membrane ◽  
Mycelium Growth ◽  
Oxidoreductase Activity ◽  
Wood Treatment

Abstract Background Lenzites gibbosa is a common white-rot fungus of Polyporaceae in the cold temperate zone which cause spongy white decay of wood. Results In this study, the lignin degradation pathway of L. gibbosa at 5-time points under wood treatment was studied by RNA-Req technology. A total of 5232 DEGs were identified from 15 libraries. In 0–3 days, the mycelia are in the adaptive stage, and the mycelia began to proliferate within 3–5 days. After 5 days, the number of DGEs decreased significantly, the mycelium growth entered the platform stage, and the life activity was basically stable. In the secondary metabolism, oxidoreductase such as laccase, 2-oxoglutarate-Fe (II) type oxidoreductase, peroxisomal hydratase-dehydrogenase, dual-functional monooxygenase dominated and increased steadily, and manganese peroxidase appeared in the middle stage. With the accumulation of lignin intermediate products, P450 and ABC transporters were from the inhibition to the activation. Weighted gene co-expression network analysis (WGCNA) showed that among the 24 modules 6 modules were significantly correlated with laccase activity and the most correlated were turquoise and blue module. The central hub genes were also identified, including gene_7458, gene_61, gene_7458, gene_1741, gene_11087 which were consistent with the DGEs. These genes have high connectivity, module membership, and gene significance in the module. The enrichment analysis of GO and KEGG pathway indicated that the genes involved in cell cycle, citrate cycle (TCA cycle), nicotinate and nicotinamide metabolism, mitochondrial outer membrane, succinate dehydrogenase, carboxypeptidase and exopeptidase activity, flavin adenine dinucleotide binding, oxidoreductase activity, acting on the CH-CH group of donors, quinone or related compound as acceptor pathway were highly related to laccase synthesis pathway. Construction of gene co-expression network and hypothetical L. gibbosa laccase synthesis pathway. Conclusion This study focused on the screening of L. gibbosa degradation of lignin-related genes. And, this is the first study reporting co-expression patterns of a gene network in L. gibbosa laccase activity after wood treatment which is helpful to understand the synthesis pathway of laccase and improve the activity of laccase, which can be used to increase the rate of lignin degradation by L. gibbosa in the further.

scholarly journals Cerrena unicolor Laccases, Genes Expression and Regulation of Activity

Biomolecules ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 468
Author(s):  
Anna Pawlik ◽  
Beata Ciołek ◽  
Justyna Sulej ◽  
Andrzej Mazur ◽  
Przemysław Grela ◽  
...  
Keyword(s):  
Laccase Activity ◽  
Environmental Changes ◽  
Specific Activity ◽  
Electrochemical Analysis ◽  
White Rot ◽  
White Rot Fungus ◽  
Cerrena Unicolor ◽  
Genes Expression ◽  
Regulation Of Activity ◽  
Laccase Genes

A white rot fungus Cerrena unicolor has been identified as an important source of laccase, unfortunately regulation of this enzyme genes expression is poorly understood. Using 1D and 2D PAGE and LC-MS/MS, laccase isoenzymes were investigated in the liquid filtrate of C. unicolor culture. The level of expression of laccase genes was measured using qPCR. The elevated concentrations of copper and manganese in the medium caused greatest change in genes expression and three laccase transcripts were significantly affected after culture temperature was decreased from 28 to 4 °C or increased to 40 °C. The small differences in the PAGE band intensities of individual laccase proteins were also observed, indicating that given compound affect particular laccase’s transcript. Analyses of laccase-specific activity, at all tested conditions, showed the increased activities as compared to the control, suggesting that enzyme is regulated at the post-translational stage. We observed that the aspartic protease purified from C. unicolor, significantly stimulate laccase activity. Moreover, electrochemical analysis of protease-treated laccase sample had 5 times higher redox peaks. The obtained results indicate that laccases released by C. unicolor are regulated at transcriptional, translational, and at the post-translational steps of gene expression helping fungus adapt to the environmental changes.

The screening of culture condition and properties of xylanase by white-rot fungus Pleurotus ostreatus

2004 ◽  
Vol 39 (11) ◽  
pp. 1561-1566 ◽  
Author(s):  
Cai Qinnghe ◽  
Yue Xiaoyu ◽  
Niu Tiangui ◽  
Ji Cheng ◽  
Ma Qiugang
Keyword(s):  
Culture Condition ◽  
Pleurotus Ostreatus ◽  
White Rot ◽  
White Rot Fungus
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