Prevalence and Characterization of Cryptosporidium Species in Tibetan Antelope (Pantholops hodgsonii)

Cryptosporidium is an enteric apicomplexan parasite, which can infect multiple mammals including livestock and wildlife. Tibetan Antelope (Pantholops hodgsonii) is one of the most famous wildlife species, that belongs to the first class protected wild animals in China. However, it has not been known whether Tibetan Antelope is infected with Cryptosporidium so far. The objective of the present study was to determine the prevalence and characterization of Cryptosporidium species infection in Tibetan Antelope and the corresponding species by using molecular biological method. In the current study, a total of 627 fecal samples were randomly collected from Tibetan Antelope in the Tibet Autonomous Region (2019–2020), and were examined by PCR amplification of the small subunit ribosomal RNA (SSU rRNA) gene. Among 627 samples, 19 (3.03%, 19/627) were examined as Cryptosporidium-positive, with 7 (2.33%, 7/300) in females and 12 (3.67%, 12/327) in males. The analysis of SSU rRNA gene sequence suggested that only two Cryptosporidium species, namely, C. xiaoi and C. ubiquitum, were identified in this study. This is the first evidence for an existence of Cryptosporidium in Tibetan Antelope. These findings extend the host range for Cryptosporidium spp. and also provide important data support for prevention and control of Cryptosporidium infection in Tibetan Antelope.

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Prevalence and Genetic Characterization of Cryptosporidium Infection in Java Sparrows (Lonchura oryzivora) in Northern China

BioMed Research International ◽

10.1155/2017/2318476 ◽

2017 ◽

Vol 2017 ◽

pp. 1-4 ◽

Cited By ~ 2

Author(s):

Qiu-Xia Yao ◽

Xiao-Xuan Zhang ◽

Kai Chen ◽

Jian-Gang Ma ◽

Wen-Bin Zheng ◽

...

Keyword(s):

Pcr Amplification ◽

Northern China ◽

Small Subunit ◽

Rrna Gene ◽

Ssu Rrna ◽

Ssu Rrna Gene ◽

Wide Range ◽

Baseline Information ◽

And Control

Cryptosporidiosis is a cosmopolitan parasitosis that affects a wide range of hosts including birds. As information concerning Cryptosporidium in birds is limited, the present study examined the prevalence and genotypes of Cryptosporidium in Java sparrows in Beijing and Shangqiu, northern China. Three hundred and fifty fecal samples were collected from Java sparrows (Lonchura oryzivora, 225 white Java sparrows and 125 gray Java sparrows) in Beijing and Shangqiu in October 2015, and the samples were examined by PCR amplification of the small subunit ribosomal RNA (SSU rRNA) gene. The overall Cryptosporidium prevalence is 13.42% (47/350), with 16.44% (37/225) in white Java sparrows and 8.00% (10/125) in gray Java sparrows. Cryptosporidium prevalence was 9.82% (16/163) in Java sparrows from Beijing and 16.58% (31/187) in Java sparrows from Shangqiu. The prevalence of Cryptosporidium in females and males was 40.63% (26/64) and 7.34% (21/286), respectively. The Cryptosporidium prevalence in Java sparrows of different ages varied from 10.47% to 16.33%. Sequence analysis of the SSU rRNA gene revealed that all the samples represented C. baileyi. This is the first report of Cryptosporidium in gray Java sparrows in China, which extend the host range for C. baileyi. These results provide baseline information for further studies of molecular epidemiology and control of Cryptosporidium infection in poultry in China.

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Telonema antarcticum sp. nov., a common marine phagotrophic flagellate

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.63652-0 ◽

2005 ◽

Vol 55 (6) ◽

pp. 2595-2604 ◽

Cited By ~ 26

Author(s):

Dag Klaveness ◽

Kamran Shalchian-Tabrizi ◽

Helge Abildhauge Thomsen ◽

Wenche Eikrem ◽

Kjetill S. Jakobsen

Keyword(s):

Molecular Phylogeny ◽

Small Subunit ◽

Food Vacuole ◽

Rrna Gene ◽

Ssu Rrna ◽

Small Subunit Rrna ◽

Ssu Rrna Gene ◽

Structural Identity ◽

Food Particles ◽

Ssu Rrna Gene Sequence

Telonema is a widely distributed group of phagotrophic flagellates with two known members. In this study, the structural identity and molecular phylogeny of Telonema antarcticum was investigated and a valid description is proposed. Molecular phylogeny was studied using small-subunit rRNA (SSU rRNA) gene sequences. The pear-shaped cell had two subequal flagella that emerged laterally on the truncated antapical tail. One flagellum had tripartite hairs. The cell was naked, but had subsurface vesicles containing angular paracrystalline bodies of an unknown nature. A unique complex cytoskeletal structure, the subcortical lamina, was found to be an important functional and taxonomic feature of the genus. Telonema has an antero-ventral depression where food particles are ingested and then transferred to a conspicuous anterior food vacuole. The molecular phylogeny inferred from the SSU rRNA gene sequence suggested that Telonema represents an isolated and deep branch among the tubulocristate protists.

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Taxonomy, morphology and molecular systematics of three oligotrich ciliates, including a description of Apostrombidium parakielum spec. nov. (Ciliophora, Oligotrichia)

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.048314-0 ◽

2013 ◽

Vol 63 (Pt_3) ◽

pp. 1179-1191 ◽

Cited By ~ 14

Author(s):

Wen Song ◽

Jiamei Li ◽

Weiwei Liu ◽

Jiamei Jiang ◽

Khaled A. S. Al- Rasheid ◽

...

Keyword(s):

Sequence Data ◽

Novel Species ◽

Small Subunit ◽

Rrna Gene ◽

Ssu Rrna ◽

The Novel ◽

Small Subunit Rrna ◽

Ssu Rrna Gene ◽

Single Clade ◽

Ssu Rrna Gene Sequence

Three oligotrich ciliates, Apostrombidium parakielum spec. nov., Novistrombidium apsheronicum (Alekperov & Asadullayeva, 1997) Agatha, 2003 and Novistrombidium testaceum (Anigstein, 1914) Song & Bradbury, 1998 were collected from the coastal waters of China and their morphology and small-subunit rRNA (SSU rRNA) gene sequences were studied. The novel species can be recognized by the combination of its obconical body shape, 14–16 anterior and 6–8 ventral membranelles, somatic kinety in three parts and conspicuously long dorsal cilia. Based on the data obtained for this novel species, an improved diagnosis of the genus Apostrombidium is supplied. Descriptions of the population of N. apsheronicum and N. testaceum collected in this study are also provided and compared with the existing descriptions. In addition, the phylogenetic positions of these three species are inferred from their SSU rRNA gene sequence data. The results indicate that the genus Apostrombidium, the systematics of which has not previously been discussed using molecular information, clusters with Varistrombidium kielum and Omegastrombidium elegans, whereas N. testaceum and N. apsheronicum form a single clade.

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Prevalence and molecular characterization of Cryptosporidium spp. in Père David’s deer (Elaphurus davidianus) in Jiangsu, China

Revista Brasileira de Parasitologia Veterinária ◽

10.1590/s1984-29612020013 ◽

2020 ◽

Vol 29 (2) ◽

Author(s):

Si-Yang Huang ◽

Yi-Min Fan ◽

Yi Yang ◽

Yi-Jun Ren ◽

Jing-Zhi Gong ◽

...

Keyword(s):

Molecular Characterization ◽

Small Subunit ◽

Basic Knowledge ◽

Rrna Gene ◽

Ssu Rrna ◽

Ssu Rrna Gene ◽

Cryptosporidium Spp ◽

Père David’S Deer ◽

Rrna Gene Sequencing

Abstract Cryptosporidium is a zoonotic parasite that causes diarrhea in a broad range of animals, including deer. Little is known about the prevalence and genotype of Cryptosporidium spp. in Père David’s deer. In this study, 137 fecal samples from Père David’s deer were collected between July 2017 and August 2018 in the Dafeng Reserve and analyzed for Cryptosporidium spp. by nested-PCR based on the small subunit ribosomal RNA (SSU rRNA) gene, followed by sequence analyses to determine the species. The 60 kDa glycoprotein (gp60) gene was used to characterize Cryptosporidium spp. Among 137 samples, 2 (1.46%) were positive for Cryptosporidium spp. according to SSU rRNA gene sequencing results. Both samples belonged to the Cryptosporidium deer genotype, with two nucleotide deletions and one nucleotide substitution. The prevalence data and molecular characterization of this study provide basic knowledge for controlling and preventing Cryptosporidium infections in Père David’s deer in this area.

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Molecular characterization and phylogenetic relationships among microsporidian isolates infecting silkworm, Bombyx mori using small subunit rRNA (SSU-rRNA) gene sequence analysis

Acta Parasitologica ◽

10.2478/s11686-012-0051-4 ◽

2012 ◽

Vol 57 (4) ◽

Cited By ~ 4

Author(s):

B. Nath ◽

S. Gupta ◽

A. Bajpai

Keyword(s):

Sequence Analysis ◽

Gene Sequence ◽

Small Subunit ◽

Rrna Gene ◽

Ssu Rrna ◽

Rrna Gene Sequence ◽

Small Subunit Rrna ◽

Ssu Rrna Gene ◽

Gene Sequence Analysis ◽

Ssu Rrna Gene Sequence

AbstractThe life cycle, spore morphology, pathogenicity, tissue specificity, mode of transmission and small subunit rRNA (SSU-rRNA) gene sequence analysis of the five new microsporidian isolates viz., NIWB-11bp, NIWB-12n, NIWB-13md, NIWB-14b and NIWB-15mb identified from the silkworm, Bombyx mori have been studied along with type species, NIK-1s_mys. The life cycle of the microsporidians identified exhibited the sequential developmental cycles that are similar to the general developmental cycle of the genus, Nosema. The spores showed considerable variations in their shape, length and width. The pathogenicity observed was dose-dependent and differed from each of the microsporidian isolates; the NIWB-15mb was found to be more virulent than other isolates. All of the microsporidians were found to infect most of the tissues examined and showed gonadal infection and transovarial transmission in the infected silkworms. SSU-rRNA sequence based phylogenetic tree placed NIWB-14b, NIWB-12n and NIWB-11bp in a separate branch along with other Nosema species and Nosema bombycis; while NIWB-15mb and NIWB-13md together formed another cluster along with other Nosema species. NIK-1s_mys revealed a signature sequence similar to standard type species, N. bombycis, indicating that NIK-1s_mys is similar to N. bombycis. Based on phylogenetic relationships, branch length information based on genetic distance and nucleotide differences, we conclude that the microsporidian isolates identified are distinctly different from the other known species and belonging to the genus, Nosema. This SSU-rRNA gene sequence analysis method is found to be more useful approach in detecting different and closely related microsporidians of this economically important domestic insect.

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Morphology and Small Subunit (SSU) rRNA Gene Sequence of the New Brackish Water Ciliate Neobakuella flava n. g., n. sp. (Ciliophora, Spirotricha, Bakuellidae) and SSU rRNA Gene Sequences of Six Additional Hypotrichs from Korea

Journal of Eukaryotic Microbiology ◽

10.1111/j.1550-7408.2011.00561.x ◽

2011 ◽

Vol 58 (4) ◽

pp. 339-351 ◽

Cited By ~ 14

Author(s):

LIQIONG LI ◽

SADIA NAWROZ KHAN ◽

DAODE JI ◽

MANN KYOON SHIN ◽

HELMUT BERGER

Keyword(s):

Brackish Water ◽

Gene Sequence ◽

Small Subunit ◽

Rrna Gene ◽

Ssu Rrna ◽

Rrna Gene Sequence ◽

Gene Sequences ◽

Ssu Rrna Gene ◽

Ssu Rrna Gene Sequence

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Phylogenetic position of Multicilia marina and the evolution of Amoebozoa

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.63763-0 ◽

2006 ◽

Vol 56 (6) ◽

pp. 1449-1458 ◽

Cited By ~ 48

Author(s):

Sergey I. Nikolaev ◽

Cédric Berney ◽

Nikolai B. Petrov ◽

Alexandre P. Mylnikov ◽

José F. Fahrni ◽

...

Keyword(s):

Phylogenetic Analyses ◽

Strong Support ◽

Molecular Data ◽

Small Subunit ◽

Phylogenetic Position ◽

Rrna Gene ◽

Ssu Rrna ◽

Ssu Rrna Gene ◽

Slime Moulds ◽

Ssu Rrna Gene Sequence

Recent molecular phylogenetic studies have led to the erection of the phylum Amoebozoa, uniting naked and testate lobose amoebae, the mycetozoan slime moulds and amitochondriate amoeboid protists (Archamoebae). Molecular data together with ultrastructural evidence have suggested a close relationship between Mycetozoa and Archamoebae, classified together in the Conosea, which was named after the cone of microtubules that, when present, is characteristic of their kinetids. However, the relationships of conoseans to other amoebozoans remain unclear. Here, we obtained the complete small-subunit (SSU) rRNA gene sequence (2746 bp) of the enigmatic, multiflagellated protist Multicilia marina, which has formerly been classified either in a distinct phylum, Multiflagellata, or among lobose amoebae. Our study clearly shows that Multicilia marina belongs to the Amoebozoa. Phylogenetic analyses including 60 amoebozoan SSU rRNA gene sequences revealed that Multicilia marina branches at the base of the Conosea, together with another flagellated amoebozoan, Phalansterium solitarium, as well as with Gephyramoeba sp., Filamoeba nolandi and two unidentified amoebae. This is the first report showing strong support for a clade containing all flagellated amoebozoans and we discuss the position of the root of the phylum Amoebozoa in the light of this result.

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Morphological Redescriptions and Molecular Phylogeny of Three Stentor Species (Ciliophora: Heterotrichea: Stentoridae) from Korea

Zootaxa ◽

10.11646/zootaxa.4732.3.6 ◽

2020 ◽

Vol 4732 (3) ◽

pp. 435-452

Author(s):

MD ABU TAHER ◽

AHMED SALAHUDDIN KABIR ◽

SHAHED UDDIN AHMED SHAZIB ◽

MIN SEOK KIM ◽

MANN KYOON SHIN

Keyword(s):

Gene Sequence ◽

Small Subunit ◽

Rrna Gene ◽

Sequence Information ◽

Ssu Rrna ◽

Rrna Gene Sequence ◽

Cortical Granules ◽

Ssu Rrna Gene ◽

Ssu Rrna Gene Sequence

The morphologies of the three freshwater stentorid ciliates in Korea, Stentor coeruleus (Pallas, 1766); Stentor muelleri Ehrenberg, 1831, and Stentor tartari Murthy & Bai, 1974, were investigated based on live observations and protargol impregnation. The Korean population of S. tartari exhibits the following characteristics: body size 200–355 × 85–135 µm in vivo, 62–106 somatic kineties, 8–13 peristomial kineties, 110–180 adoral membranelles, mostly two macronuclear nodules and 5–18 micronuclei, reddish and colorless cortical granules and the presence of symbiotic algae. We identified S. tartari based on unique characteristics compared to close congeners. Korean populations of S. coeruleus and S. muelleri are congruent with previously described populations in most aspects of their morphologies. Here, for the first time, we report molecular gene sequence information for S. tartari. Small subunit (SSU) rRNA gene sequence-based phylogeny indicates that S. tartari, which has multiple macronuclei, forms a monophyletic group with other Stentor species having a single macronucleus. Our findings based on morphology and SSU rRNA gene sequence information corroborate the hypothesis that the elongated macronucleus evolved from the compact single or multi macronucleus state.

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Morphological and phylogenetic studies on three members of the genus Pseudochilodonopsis (Ciliophora, Cyrtophoria) isolated from brackish waters in China, including a novel species, Pseudochilodonopsis quadrivacuolata sp. nov.

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijsem.0.000580 ◽

2015 ◽

Vol 65 (Pt_12) ◽

pp. 4323-4334 ◽

Cited By ~ 6

Author(s):

Zhishuai Qu ◽

Hongbo Pan ◽

Khaled A. S. Al-Rasheid ◽

Xiaozhong Hu ◽

Shan Gao

Keyword(s):

Sequence Data ◽

Phylogenetic Analyses ◽

Molecular Data ◽

Small Subunit ◽

Dorsal Side ◽

Rrna Gene ◽

Ssu Rrna ◽

Ssu Rrna Gene ◽

Ssu Rrna Gene Sequence

Three cyrtophorian ciliates isolated from brackish biotopes in China, Pseudochilodonopsis quadrivacuolata sp. nov., Pseudochilodonopsis fluviatilis Foissner, 1988 and Pseudochilodonopsis mutabilis Foissner, 1981, were investigated using living observation and protargol-staining methods. P. quadrivacuolata sp. nov. can be characterized as follows: cell size 50–70 × 30–40 μm in vivo; body oval with posterior end rounded; four tetragonally positioned contractile vacuoles; 12–15 nematodesmal rods; five right and six left somatic kineties; terminal fragment positioned apically on dorsal side, consisting of 11–14 basal bodies; four or five fragments in preoral kinety. P. fluviatilis and P. mutabilis were generally consistent with previous descriptions. In addition, a brief revision and a key to Pseudochilodonopsis are presented. The small-subunit (SSU) rRNA gene was also sequenced to support the identification of these species. Phylogenetic analyses based on molecular data indicate that the genera Pseudochilodonopsis and Chilodonella are closely related and both are well outlined; that is, all known congeners for which SSU rRNA gene sequence data are available group together, forming the core part of the family Chilodonellidae.

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