An assessment on DNA microarray and sequence-based methods for the characterization of methicillin-susceptible Staphylococcus aureus from Nigeria

Introduction: Healthcare workers (HCWs) colonized with Staphylococcus aureus may serve as a reservoir of infection. This study was carried to determine the genetic make-up of S. aureus nasal colonizers in HCWs. Methodology: Nasal swabs were obtained from 93 HCWs and molecular characterization of identified S. aureus isolates was carried out using the StaphyType DNA microarray (Alere Technologies GmbH, Jena, Germany). Results: Twenty-nine HCWs (31%) were colonized with S. aureus (MSSA = 23; MRSA = 6). Thus the overall MRSA carriage rate was 6.5% (n/N = 6/93) and 20.7% (n/N = 6/29) of those colonized with S. aureus harboured MRSA. The S. aureus isolates belonged to 16 clonal complexes (CC). MSSA isolates included three each for CC15, CC188, ST2867; two each for CC5, CC97, CC367 as well as one each for CC1, CC8, CC30, CC45, CC101, CC121, ST291/813 and CC1153. The staphylococcal cassette chromosome recombinase genes ccrA-1; ccrB-1 and the fusidic acid resistance gene (fusC) were present in two MSSA isolates (CC1 and CC8). The six MRSA isolates included CC5-MRSA-[VI+fusC] (n = 2); one each of CC5-MRSA-V; CC22-MRSA-IV (tst1+); CC80-MRSA-IV [pvl+] (“European CA-MRSA Clone”) and CC97-MRSA-[V+fusC]. Conclusion: There is wide clonal diversity of S. aureus colonizers with associated high MRSA carriage among the HCWs. The presence of genetically stable MSSA isolates with the capability to transform into MRSA isolates is of concern.

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Validation of Virulence and Epidemiology DNA Microarray for Identification and Characterization of Staphylococcus aureus Isolates

Journal of Clinical Microbiology ◽

10.1128/jcm.02453-07 ◽

2008 ◽

Vol 46 (5) ◽

pp. 1620-1627 ◽

Cited By ~ 21

Author(s):

R. P. Spence ◽

V. Wright ◽

D. A. A. Ala-Aldeen ◽

D. P. Turner ◽

K. G. Wooldridge ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Dna Microarray ◽

Identification And Characterization

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Microarray-Based Characterization of Staphylococcus aureus Isolates Obtained from Chicken Carcasses

Journal of Food Protection ◽

10.4315/0362-028x.jfp-13-009 ◽

2013 ◽

Vol 76 (8) ◽

pp. 1471-1474 ◽

Cited By ~ 7

Author(s):

REBECCA EBNER ◽

SOPHIA JOHLER ◽

HENNA-MARIA SIHTO ◽

ROGER STEPHAN ◽

CLAUDIO ZWEIFEL

Keyword(s):

Staphylococcus Aureus ◽

Dna Microarray ◽

Clonal Complex ◽

Methicillin Resistance ◽

High Similarity ◽

Spa Typing ◽

Capsule Type ◽

Genes Encoding ◽

Contamination Routes

A total of 34 Staphylococcus aureus isolates from flock-wise pooled chicken neck skin samples collected at two abattoirs during slaughter were characterized with DNA microarray analysis and spa typing. The 20 isolates from abattoir A all belonged to clonal complex (CC) 12 and spa type t160. Of the 14 isolates from abattoir B, 7 belonged to CC5–t3478, 5 to CC12–t160, 1 to CC45–t040, and 1 to CC101–t056. Of the various resistance-associated genes tested, only blaZ/R/I (6 isolates of CC12 and CC101 from abattoir B), sdrM (n = 34), fosB (n = 33), and qacC (n = 22) were detected. None of the isolates harbored genes conferring methicillin resistance. In terms of genes encoding enterotoxins, seb (all isolates of CC12), egc (seg, sei, selm, seln, selo, selu; all isolates of CC5 and CC45), and sea (14 isolates of CC12 and 1 isolate of CC5) were found. In addition, all isolates harbored genes for intracellular adhesion proteins (icaA/C/D) and were positive for cap5 or cap8 (capsule type 5 or 8). Comparison of DNA microarray profiles identified four categories comprising (i) all isolates of CC12, (ii) all isolates of CC5, (iii) the CC45 isolate, and (iv) the CC101 isolate. The high similarity of the isolates from abattoir A could indicate contamination of chicken carcasses with S. aureus persisting on the slaughter equipment, but further investigations are required to elucidate potential contamination routes.

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Genotyping and DNA microarray based characterization of Staphylococcus aureus isolates from rabbit carcasses

Meat Science ◽

10.1016/j.meatsci.2015.11.002 ◽

2016 ◽

Vol 112 ◽

pp. 86-89 ◽

Cited By ~ 12

Author(s):

Axel Merz ◽

Roger Stephan ◽

Sophia Johler

Keyword(s):

Staphylococcus Aureus ◽

Dna Microarray

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Phylogeny Characterization of Seb Gene Encoding Enterotoxins in Staphylococcus aureus Isolated from Raw Milk and Cheese

International Journal of Drug Delivery Technology ◽

10.25258/ijddt.v9i3.13 ◽

2019 ◽

Vol 9 (o3) ◽

Author(s):

Fatima N. Aziz ◽

Laith Abdul Hassan Mohammed-Jawad

Keyword(s):

Staphylococcus Aureus ◽

Food Poisoning ◽

Raw Milk ◽

Staphylococcal Enterotoxin B ◽

Human Pathogen ◽

Conventional Pcr ◽

Biochemical Tests ◽

Specific Primers ◽

Gene Encoding

Food poisoning due to the bacteria is a big global problem in economically and human's health. This problem refers to an illness which is due to infection or the toxin exists in nature and the food that use. Milk is considered a nutritious food because it contains proteins and vitamins. The aim of this study is to detect and phylogeny characterization of staphylococcal enterotoxin B gene (Seb). A total of 200 milk and cheese samples were screened. One hundred ten isolates of Staphylococcus aureus pre-confirmed using selective and differential media with biochemical tests. Genomic DNA was extracted from the isolates and the SEB gene detects using conventional PCR with specific primers. Three staphylococcus aureus isolates were found to be positive for Seb gene using PCR and confirmed by sequencing. Sequence homology showed variety range of identity starting from (100% to 38%). Phylogenetic tree analyses show that samples (6 and 5) are correlated with S. epidermidis. This study discovered that isolates (A6-RLQ and A5-RLQ) are significantly clustered in a group with non- human pathogen Staphylococcus agnetis.

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