scholarly journals Evaluation and Optimization of Methods for Generating High-Resolution Retinotopic Maps Using Visual Cortex Voltage-Sensitive Dye Imaging

2021 ◽  
Vol 15 ◽  
Author(s):  
Ori Carmi ◽  
Adi Gross ◽  
Nadav Ivzan ◽  
Lamberto La Franca ◽  
Nairouz Farah ◽  
...  
Keyword(s):  
Visual Cortex ◽  
High Resolution ◽  
Voltage Sensitive Dye ◽  
Projection System ◽  
Retinal Stimulation ◽  
Retinotopic Maps ◽  
Analysis Methods ◽  
Silhouette Index ◽  
Cortical Responses ◽  
Voltage Sensitive Dye Imaging

The localization and measurement of neuronal activity magnitude at high spatial and temporal resolution are essential for mapping and better understanding neuronal systems and mechanisms. One such example is the generation of retinotopic maps, which correlates localized retinal stimulation with the corresponding specific visual cortex responses. Here we evaluated and compared seven different methods for extracting and localizing cortical responses from voltage-sensitive dye imaging recordings, elicited by visual stimuli projected directly on the rat retina by a customized projection system. The performance of these methods was evaluated both qualitatively and quantitatively by means of two cluster separation metrics, namely, the (adjusted) Silhouette Index (SI) and the (adjusted) Davies-Bouldin Index (DBI). These metrics were validated using simulated data, which showed that Temporally Structured Component Analysis (TSCA) outperformed all other analysis methods for localizing cortical responses and generating high-resolution retinotopic maps. The analysis methods, as well as the use of cluster separation metrics proposed here, can facilitate future research aiming to localize specific activity at high resolution in the visual cortex or other brain areas.

scholarly journals Rapid and Precise Retinotopic Mapping of the Visual Cortex Obtained by Voltage-Sensitive Dye Imaging in the Behaving Monkey

2007 ◽  
Vol 98 (2) ◽  
pp. 1002-1014 ◽  
Author(s):  
Zhiyong Yang ◽  
David J. Heeger ◽  
Eyal Seidemann
Keyword(s):  
Visual Cortex ◽  
Traveling Wave ◽  
High Speed ◽  
Visual Space ◽  
Submillimeter Range ◽  
Population Activity ◽  
Voltage Sensitive Dye ◽  
Retinotopic Mapping ◽  
Retinotopic Maps ◽  
Voltage Sensitive Dye Imaging

Retinotopy is a fundamental organizing principle of the visual cortex. Over the years, a variety of techniques have been used to examine it. None of these techniques, however, provides a way to rapidly characterize retinotopy, at the submillimeter range, in alert, behaving subjects. Voltage-sensitive dye imaging (VSDI) can be used to monitor neuronal population activity at high spatial and temporal resolutions. Here we present a VSDI protocol for rapid and precise retinotopic mapping in the behaving monkey. Two monkeys performed a fixation task while thin visual stimuli swept periodically at a high speed in one of two possible directions through a small region of visual space. Because visual space is represented systematically across the cortical surface, each moving stimulus produced a traveling wave of activity in the cortex that could be precisely measured with VSDI. The time at which the peak of the traveling wave reached each location in the cortex linked this location with its retinotopic representation. We obtained detailed retinotopic maps from a region of about 1 cm2 over the dorsal portion of areas V1 and V2. Retinotopy obtained during <4 min of imaging had a spatial precision of 0.11–0.19 mm, was consistent across experiments, and reliably predicted the locations of the response to small localized stimuli. The ability to rapidly obtain precise retinotopic maps in behaving monkeys opens the door for detailed analysis of the relationship between spatiotemporal dynamics of population responses in the visual cortex and perceptually guided behavior.

scholarly journals Voltage-sensitive dye imaging of collinear patterns in the visual cortex of a behaving monkey

2010 ◽  
Vol 7 (9) ◽  
pp. 75-75
Author(s):  
E. Meirovithz ◽  
Y. Bonneh ◽  
U. Werner-Reiss ◽  
I. Ayzenshtat ◽  
G. Saban ◽  
...  
Keyword(s):  
Visual Cortex ◽  
Voltage Sensitive Dye ◽  
Voltage Sensitive Dye Imaging

Models and Measurements of Functional Maps in V1

2008 ◽  
Vol 99 (6) ◽  
pp. 2745-2754 ◽  
Author(s):  
Naoum P. Issa ◽  
Ari Rosenberg ◽  
T. Robert Husson
Keyword(s):  
Visual Cortex ◽  
High Resolution ◽  
Spatial Frequency ◽  
Temporal Frequency ◽  
Reasonable Accuracy ◽  
Spatiotemporal Filtering ◽  
Population Responses ◽  
Moving Images ◽  
Complex Stimuli ◽  
Cortical Responses

The organization of primary visual cortex has been heavily studied for nearly 50 years, and in the last 20 years functional imaging has provided high-resolution maps of its tangential organization. Recently, however, the usefulness of maps like those of orientation and spatial frequency (SF) preference has been called into question because they do not, by themselves, predict how moving images are represented in V1. In this review, we discuss a model for cortical responses (the spatiotemporal filtering model) that specifies the types of cortical maps needed to predict distributed activity within V1. We then review the structure and interrelationships of several of these maps, including those of orientation, SF, and temporal frequency preference. Finally, we discuss tests of the model and the sufficiency of the requisite maps in predicting distributed cortical responses. Although the spatiotemporal filtering model does not account for all responses within V1, it does, with reasonable accuracy, predict population responses to a variety of complex stimuli.

Investigation of detectability of a biomedical photonic LSI (BpLSI) device for voltage-sensitive dye imaging of mouse visual cortex

2011 ◽  
Vol 71 ◽  
pp. e205
Author(s):  
Takuma Kobayashi ◽  
Mayumi Motoyama ◽  
Sawadsaringkam Yosmongkol ◽  
Ayato Tagawa ◽  
Toshihiko Noda ◽  
...  
Keyword(s):  
Visual Cortex ◽  
Voltage Sensitive Dye ◽  
Mouse Visual Cortex ◽  
Voltage Sensitive Dye Imaging

scholarly journals Cortical long-range interactions embed statistical knowledge of natural sensory input: a voltage-sensitive dye imaging study

F1000Research ◽  
2013 ◽  
Vol 2 ◽  
pp. 51 ◽  
Author(s):  
Selim Onat ◽  
Dirk Jancke ◽  
Peter König
Keyword(s):  
Visual Cortex ◽  
Primary Visual Cortex ◽  
Sensory Input ◽  
Synergistic Effects ◽  
Temporal Structure ◽  
Imaging Study ◽  
Simultaneous Presentation ◽  
Voltage Sensitive Dye ◽  
Dynamic Stimuli ◽  
Voltage Sensitive Dye Imaging

How is contextual processing as demonstrated with simplified stimuli, cortically enacted in response to ecologically relevant complex and dynamic stimuli? Using voltage-sensitive dye imaging, we captured mesoscopic population dynamics across several square millimeters of cat primary visual cortex. By presenting natural movies locally through either one or two adjacent apertures, we show that simultaneous presentation leads to mutual facilitation of activity. These synergistic effects were most effective when both movie patches originated from the same natural movie, thus forming a coherent stimulus in which the inherent spatio-temporal structure of natural movies were preserved in accord with Gestalt principles of perceptual organization. These results suggest that natural sensory input triggers cooperative mechanisms that are imprinted into the cortical functional architecture as early as in primary visual cortex.

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