Background:Hundreds of investigations examining biomechanical outcomes of various prostheses have been completed, but one question remains unanswered: how much time should an amputee be given to accommodate to a new prosthesis prior to biomechanical testing?Objective:To examine the literature for accommodation time given during biomechanical investigations to determine whether consensus exists.Study design:Systematic review.Methods:A systematic search was completed on 7 January 2016 using PubMed and Scopus.Results:The search resulted in 156 investigations. Twenty-eight studies did not provide an accommodation or were unclear (e.g. provided a “break in period”), 5 studies tested their participants more than once, 25 tested only once and on the same day participants received a new prosthesis (median (range): above-knee: 60 (10–300) min; below-knee: 18 (5–300) min), and 98 tested once and gave a minimum of 1 day for accommodation (hip: 77 (60–180) days; above-knee: 42 (1–540) days; below-knee: 21 (1–475) days).Conclusion:The lack of research specifically examining accommodation and the high variability in this review’s results indicates that it remains undecided how much accommodation is necessary. There is a need for longitudinal biomechanical investigations to determine how outcomes change as amputees accommodate to a new prosthesis.Clinical relevanceThe results of this review indicate that little research has been done regarding lower-limb amputees accommodating to a new prosthesis. Improper accommodation could lead to increased variability in results, results that are not reflective of long-term use, and could cause clinicians to make inappropriate decisions regarding a prosthesis.