Genotoxicity of PM2.5 and PM1.0 Particulates on Human Peripheral Blood Lymphocytes in Manila, Philippines

Urban air quality is increasingly being studied as a fraction of the world’s population is living in megacities. In this study, particulate matter (PM) along Taft Avenue, Manila, the Philippines, is investigated in terms of its ability to induce genetic damage in human peripheral blood lymphocytes (PBLs). Size-segregated roadside air samples were obtained from 2015–2017 near a university gate and analyzed using in vitro micronucleus (MN) and cytokinesis-block proliferation tests. While cellular proliferation was unaffected by 0–0.1 kg/m3 of PM1.0 and PM2.5, PBL cells treated with PM2.5 displayed a significantly higher micronucleus count (p = 0.03) compared to the cells treated with PM1.0. Atomic absorption spectroscopy revealed greater amounts of Cd, Ca, Pb, K, Na, and Zn in PM2.5 compared to PM1.0. The results indicate that the differences in composition of the two size fractions of air particulates are associated with their genotoxicities.

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Genotoxicity of PM2.5 and PM1.0 Particulates on Human Peripheral Blood Lymphocytes in Manila, Philippines

10.20944/preprints202111.0022.v1 ◽

2021 ◽

Author(s):

Ma. Katrina Gale Estonilo ◽

Joedith Anne Cazeñas ◽

Carlos Josef Villafuerte ◽

Custer Deocaris ◽

Gloriamaris Caraos ◽

...

Keyword(s):

Peripheral Blood ◽

Cellular Proliferation ◽

Human Peripheral Blood ◽

Peripheral Blood Lymphocytes ◽

Genetic Damage ◽

Air Samples ◽

Blood Lymphocytes ◽

Human Peripheral Blood Lymphocytes ◽

The University

Urban air quality is increasingly being studied as a fraction of the world's population is now living in megacities. In this study, particulate matter (PM) along Taft Avenue, Manila, Philippines, is investigated in terms of its ability to induce genetic damage on human peripheral blood lymphocytes (PBL). Size-segregated roadside air samples were obtained from 2015-2016 near the university gate and analyzed using in vitro micronucleus and cytokinesis-block proliferation tests. While cellular proliferation was unaffected by 0 – 0.1 kg·m-3 of PM1.0 and PM2.5, PBL cells treated with PM2.5 displayed significantly higher micronucleus count (p = 0.03) compared to the cells treated with PM1.0. Atomic Absorption Spectroscopy revealed greater amounts of Cd, Ca, Pb, K, Na, and Zn in PM2.5 compared to PM1.0. The results indicate the differences in composition of the two size fractions of air particulates are associated with their genotoxicities.

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In Vitro Genotoxicity of Wastewaters from the Town of Settat, Morocco

Archives of Industrial Hygiene and Toxicology ◽

10.2478/10004-1254-60-2009-1926 ◽

2009 ◽

Vol 60 (3) ◽

pp. 289-296 ◽

Cited By ~ 6

Author(s):

Jaouad El Asslouj ◽

Loubna Amahdar ◽

Kamal Glouib ◽

Sanae Kholtei ◽

Namira El Amrani Paaza ◽

...

Keyword(s):

Peripheral Blood ◽

Cellular Proliferation ◽

Human Peripheral Blood ◽

Peripheral Blood Lymphocytes ◽

Proliferation Index ◽

Preliminary Treatment ◽

Blood Lymphocytes ◽

Human Peripheral Blood Lymphocytes ◽

The Town

In Vitro Genotoxicity of Wastewaters from the Town of Settat, MoroccoIn recent years, the town of Settat has seen a considerable industrial growth, which has resulted in increased environmental pollution. This includes pollution by household and industrial wastewaters, which are released into the Boumoussa River without any preliminary treatment. The river valley crosses the community of Mzamza 8 km to the north of the town. Years of drought forced members of the community to use this polluted ground water for irrigation and put themselves and the environment at risk.The aim of this study was to determine the physicochemical and metal profile of Settat wastewaters and to assess their impact on the water table. The second objective was to investigate the genotoxic potential of wastewater on human peripheral blood lymphocytes in vitro, using the micronucleus test and cellular proliferation index.This study demonstrated significant pollution of Boumoussa valley groundwater and of the local wells. Sampled water induced a clear increase in the frequency of micronucleated cells and a lower cell proliferation in human peripheral blood lymphocytes in vitro.

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