scholarly journals Characterization of Bottlenose Dolphin (Tursiops truncatus) Sperm Based on Morphometric Traits

Biology ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 355
Author(s):  
María del Carmen Fuentes-Albero ◽  
Silvia Abril Sánchez ◽  
José Luis Ros-Santaella ◽  
Eliana Pintus ◽  
Chiara Luongo ◽  
...  
Keyword(s):  
Bottlenose Dolphin ◽  
Tursiops Truncatus ◽  
Storage Temperature ◽  
Storage Period ◽  
Serum Testosterone ◽  
Head Width ◽  
Adult Males ◽  
Morphometric Traits ◽  
Sperm Morphometry ◽  
Principal Piece

Bottlenose dolphin (Tursiops truncatus) males follow many reproductive strategies to ensure their paternity. However, little is known about the sperm traits, including morphometric features, that contribute to their reproductive success. Our aim was to study dolphin sperm morphometry (a total of 13 parameters) in two adult males to evaluate (i) presumptive sperm subpopulations, (ii) the correlation of sperm morphometry with testosterone levels and (iii) the effect of refrigerated storage on the sperm morphometry. Sperm populations were classified into four principal components (PCs) based on morphometry (>94% of cumulative variance). The PCs clustered into two different sperm subpopulations, which differed between males. Furthermore, the levels of serum testosterone were positively correlated with the length of the midpiece but negatively correlated with head width and the principal piece, flagellum and total sperm lengths. Most of the sperm morphometric parameters changed during the storage period (day 1 vs. day 7), but only the principal piece length was affected by the storage temperature (5 °C vs. 15 °C). This is the first study to identify dolphin sperm subpopulations based on morphometry and the influence of serum testosterone and refrigeration on sperm morphometry.

scholarly journals Malignant Seminoma with Metastasis, Sertoli Cell Tumor, and Pheochromocytoma in a Spotted Dolphin (Stenella frontalis) and Malignant Seminoma with Metastasis in a Bottlenose Dolphin (Tursiops truncatus)

2005 ◽  
Vol 42 (3) ◽  
pp. 357-359 ◽  
Author(s):  
J. S. Estep ◽  
R. E. Baumgartner ◽  
F. Townsend ◽  
D. A. Pabst ◽  
W. A. Mclellan ◽  
...  
Keyword(s):  
Sertoli Cell ◽  
Bottlenose Dolphin ◽  
Tursiops Truncatus ◽  
Bottlenose Dolphins ◽  
Cell Tumor ◽  
Adult Males ◽  
Sertoli Cell Tumor ◽  
Stenella Frontalis ◽  
First Report ◽  
Spotted Dolphin

Seminoma with metastasis was diagnosed in a spotted dolphin ( Stenella frontalis) and an Atlantic bottlenose dolphin ( Tursiops truncatus). Sertoli cell tumor and pheochromocytoma were also diagnosed in the spotted dolphin. The spotted and bottlenose dolphins were adult males that stranded and died on the coasts of northwest Florida and southeast North carolina, respectively. Neoplasia is infrequently reported in cetaceans. This is the first report of seminoma, Sertoli cell tumor, and pheochromocytoma in a dolphin, the first report of three distinct neoplasms in a dolphin, and one of the few reports of malignant neoplasia in dolphins.

Storage of Human Blood Platelets

1974 ◽  
Vol 32 (02/03) ◽  
pp. 405-416 ◽  
Author(s):  
M. R Hardeman ◽  
Carina J L. Heynens
Keyword(s):  
Storage Temperature ◽  
Platelet Rich Plasma ◽  
Blood Platelets ◽  
Storage Period ◽  
Serotonin Uptake ◽  
Hypotonic Shock ◽  
Glycolytic Intermediates

SummaryStorage experiments were performed at 4°, 25° and 37° C with platelet-rich plasma under sterile conditions. In some experiments also the effect of storing platelets at 4° C in whole blood was investigated.Before, during and after three days of storage, the platelets were tested at 37° C for their serotonin uptake and response to hypotonic shock. In addition some glycolytic intermediates were determined.A fair correlation was noticed between the serotonin uptake and hypotonic shock experiments. Both parameters were best maintained at 25° C. Also platelet counting, performed after the storage period, indicated 25° C as the best storage temperature. Determination of glycolytic intermediates did not justify any conclusion regarding the optimal storage temperature. Of the various anticoagulants studied, ACD and heparin gave the best results as to the serotonin uptake and hypotonic shock response, either with fresh or stored platelets. The use of EDTA resulted in the lowest activity, especially after storage.The results of these storage experiments in vitro, correspond well with those in vivo reported in the literature.

First Leucistic Bottlenose Dolphin (Tursiops truncatus) Sighting Registered in the Gulf of California, Mexico

2019 ◽  
Vol 45 (5) ◽  
pp. 507-512
Author(s):  
Héctor Pérez-Puig ◽  
Gisela Heckel ◽  
Lorayne Meltzer
Keyword(s):  
Bottlenose Dolphin ◽  
Tursiops Truncatus ◽  
Gulf Of California

Anatomical Description of an Infant Bottlenose Dolphin (Tursiops truncatus) Brain from Magnetic Resonance Images

2004 ◽  
Vol 30 (2) ◽  
pp. 315-326 ◽  
Author(s):  
Lori Marino ◽  
Keith Sudheimer ◽  
D. Ann Pabst ◽  
William A. Mclellan ◽  
Saima Arshad ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Bottlenose Dolphin ◽  
Tursiops Truncatus ◽  
Magnetic Resonance Images

Historic foraging ecology of the endangered Lahille's bottlenose dolphin (Tursiops truncatus gephyreus) inferred by stable isotopes

2021 ◽  
pp. 107393
Author(s):  
Andrea Campos-Rangel ◽  
Ricardo Bastida ◽  
Pedro Fruet ◽  
Paula Laporta ◽  
Humberto Luis Cappozzo ◽  
...  
Keyword(s):  
Stable Isotopes ◽  
Bottlenose Dolphin ◽  
Tursiops Truncatus ◽  
Foraging Ecology

scholarly journals Changes in Particle Size, Sedimentation, and Protein Microstructure of Ultra-High-Temperature Skim Milk Considering Plasmin Concentration and Storage Temperature

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2339
Author(s):  
So-Yul Yun ◽  
Jee-Young Imm
Keyword(s):  
Particle Size ◽  
High Temperature ◽  
Storage Temperature ◽  
Whey Proteins ◽  
Skim Milk ◽  
Storage Period ◽  
Sediment Analysis ◽  
Sediment Formation ◽  
And Storage ◽  
Ultra High Temperature

Age gelation is a major quality defect in ultra-high-temperature (UHT) pasteurized milk during extended storage. Changes in plasmin (PL)-induced sedimentation were investigated during storage (23 °C and 37 °C, four weeks) of UHT skim milk treated with PL (2.5, 10, and 15 U/L). The increase in particle size and broadening of the particle size distribution of samples during storage were dependent on the PL concentration, storage period, and storage temperature. Sediment analysis indicated that elevated storage temperature accelerated protein sedimentation. The initial PL concentration was positively correlated with the amount of protein sediment in samples stored at 23 °C for four weeks (r = 0.615; p < 0.01), whereas this correlation was negative in samples stored at 37 °C for the same time (r = −0.358; p < 0.01) due to extensive proteolysis. SDS-PAGE revealed that whey proteins remained soluble over storage at 23 °C for four weeks, but they mostly disappeared from the soluble phase of PL-added samples after two weeks’ storage at 37 °C. Transmission electron micrographs of PL-containing UHT skim milk during storage at different temperatures supported the trend of sediment analysis well. Based on the Fourier transform infrared spectra of UHT skim milk stored at 23 °C for three weeks, PL-induced particle size enlargement was due to protein aggregation and the formation of intermolecular β-sheet structures, which contributed to casein destabilization, leading to sediment formation.

Sign in / Sign up

Export Citation Format

Share Document