scholarly journals An Efficient Agrobacterium Mediated Transformation of Pineapple with GFP-Tagged Protein Allows Easy, Non-Destructive Screening of Transgenic Pineapple Plants

Biomolecules ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 617 ◽  
Author(s):  
S. V. G. N. Priyadarshani ◽  
Hanyang Cai ◽  
Qiao Zhou ◽  
Yanhui Liu ◽  
Yan Cheng ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Reporter Gene ◽  
Molecular Breeding ◽  
Growth Conditions ◽  
Root Cells ◽  
Tropical Fruit ◽  
Agrobacterium Mediated Transformation ◽  
Labor Requirement ◽  
Time Required ◽  
Non Destructive

Quite a few studies have been conducted to improve the Agrobacterium-mediated transformation of pineapple, which is the second most important commercial tropical fruit crop worldwide. However, pineapple transformation remains challenging, due to technical difficulties, the lengthy regeneration process, and a high labor requirement. There have not been any studies specifically addressing the introduction of GFP-tagged genes into pineapples through Agrobacterium-mediated transformation, which would enable easy, non-destructive expression detection. It would also allow expression localization at the organelle level, which is not possible with GUS a reporter gene that encodes β-glucuronidase or a herbicide resistance reporter gene. Here, we report a method for the introduction of GFP-tagged genes into pineapples through Agrobacterium-mediated transformation. We used embryonic calli for transformation, and plants were regenerated through somatic embryogenesis. In this study, we optimized the incubation time for Agrobacterium infection, the co-cultivation time, the hygromycin concentration for selection, and the callus growth conditions after selection. Our strategy reduced the time required to obtain transgenic plants from 7.6 months to 6.1 months. The expression of GFP-tagged AcWRKY28 was observed in the nuclei of transgenic pineapple root cells. This method allows easy, non-destructive expression detection of transgenic constructs at the organelle level. These findings on pineapple transformation will help accelerate pineapple molecular breeding efforts to introduce new desirable traits.

scholarly journals MaMAPK3-MaICE1-MaPOD P7 pathway, a positive regulator of cold tolerance in banana

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Jie Gao ◽  
Tongxin Dou ◽  
Weidi He ◽  
Ou Sheng ◽  
Fangcheng Bi ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Cold Tolerance ◽  
Molecular Breeding ◽  
Cold Resistance ◽  
Oxidoreductase Activity ◽  
Tropical Fruit ◽  
Over Expression ◽  
Cavendish Banana ◽  
Cold Tolerant ◽  
Necrotic Symptoms

Abstract Background Banana is a tropical fruit with a high economic impact worldwide. Cold stress greatly affects the development and production of banana. Results In the present study, we investigated the functions of MaMAPK3 and MaICE1 involved in cold tolerance of banana. The effect of RNAi of MaMAPK3 on Dajiao (Musa spp. ‘Dajiao’; ABB Group) cold tolerance was evaluated. The leaves of the MaMAPK3 RNAi transgenic plants showed wilting and severe necrotic symptoms, while the wide-type (WT) plants remained normal after cold exposure. RNAi of MaMAPK3 significantly changed the expressions of the cold-responsive genes, and the oxidoreductase activity was significantly changed in WT plants, while no changes in transgenic plants were observed. MaICE1 interacted with MaMAPK3, and the expression level of MaICE1 was significantly decreased in MaMAPK3 RNAi transgenic plants. Over-expression of MaICE1 in Cavendish banana (Musa spp. AAA group) indicated that the cold resistance of transgenic plants was superior to that of the WT plants. The POD P7 gene was significantly up-regulated in MaICE1-overexpressing transgenic plants compared with WT plants, and the POD P7 was proved to interact with MaICE1. Conclusions Taken together, our work provided new and solid evidence that MaMAPK3-MaICE1-MaPOD P7 pathway positively improved the cold tolerance in monocotyledon banana, shedding light on molecular breeding for the cold-tolerant banana or other agricultural species.

Somatic embryogenesis and Agrobacterium-mediated transformation of Gladiolus hybridus cv. ‘Advance Red’

2014 ◽  
Vol 120 (2) ◽  
pp. 717-728 ◽  
Author(s):  
Jian Wu ◽  
Chen Liu ◽  
Shanshan Seng ◽  
Muhammad Ali Khan ◽  
Juanjuan Sui ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Agrobacterium Mediated Transformation ◽  
Gladiolus Hybridus

scholarly journals SOMATIC EMBRYOGENESIS FOR AGROBACTERIUM MEDIATED TRANSFORMATION OF TOMATO- SOLANUM LYCOPERSICUM L.

2011 ◽  
Vol 3 (2) ◽  
pp. 72-79 ◽  
Author(s):  
SHANMUGAM GIRIJA ◽  
◽  
KILANKAJE ASHAKIRAN ◽  
MAHADEVAN CHIDAMBARESWARAN ◽  
VAITHIYANATHAN GOVINDASAMY ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Solanum Lycopersicum ◽  
Agrobacterium Mediated Transformation ◽  
Solanum Lycopersicum L

scholarly journals Simultaneous transmission and reception on all elements of an array: binary code excitation

2019 ◽  
Vol 475 (2225) ◽  
pp. 20180831 ◽  
Author(s):  
Julio A. Isla ◽  
Frederic B. Cegla
Keyword(s):  
Binary Code ◽  
Signal To Noise Ratio ◽  
Ultrasound Probe ◽  
Data Throughput ◽  
Element System ◽  
Order Of Magnitude ◽  
Time Required ◽  
Pulse Echo ◽  
First Time ◽  
Non Destructive

Pulse-echo arrays are used in radar, sonar, seismic, medical and non-destructive evaluation. There is a trend to produce arrays with an ever-increasing number of elements. This trend presents two major challenges: (i) often the size of the elements is reduced resulting in a lower signal-to-noise ratio (SNR) and (ii) the time required to record all of the signals that correspond to every transmit–receive path increases. Coded sequences with good autocorrelation properties can increase the SNR while orthogonal sets can be used to simultaneously acquire all of the signals that correspond to every transmit–receive path. However, a central problem of conventional coded sequences is that they cannot achieve good autocorrelation and orthogonality properties simultaneously due to their length being limited by the location of the closest reflectors. In this paper, a solution to this problem is presented by using coded sequences that have receive intervals. The proposed approach can be more than one order of magnitude faster than conventional methods. In addition, binary excitation and quantization can be employed, which reduces the data throughput by roughly an order of magnitude and allows for higher sampling rates. While this concept is generally applicable to any field, a 16-element system was built to experimentally demonstrate this principle for the first time using a conventional medical ultrasound probe.

scholarly journals Efficient Agrobacterium-mediated transformation and the usefulness of a synthetic GFP reporter gene in leading varietien of Japonica rice.

1999 ◽  
Vol 16 (5) ◽  
pp. 397-401 ◽  
Author(s):  
Fujio HASHIZUME ◽  
Tohru TSUCHIYA ◽  
Masashi UGAKI ◽  
Yasuo NIWA ◽  
Naoki TACHIBANA ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Japonica Rice ◽  
Agrobacterium Mediated Transformation ◽  
Gfp Reporter ◽  
Gfp Reporter Gene
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