scholarly journals Investigation of Biofilms Formed on Steelmaking Slags in Marine Environments for Water Depuration

2020 ◽  
Vol 21 (18) ◽  
pp. 6945
Author(s):  
Akiko Ogawa ◽  
Reiji Tanaka ◽  
Nobumitsu Hirai ◽  
Tatsuki Ochiai ◽  
Ruu Ohashi ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Bacterial Communities ◽  
Steelmaking Slag ◽  
Marine Environments ◽  
Next Generation ◽  
Ise Bay ◽  
Steelmaking Slags ◽  
Sequencing Technique ◽  
Sulfur Oxidizing ◽  
Generation Sequencing

Steelmaking slags are a promising resource as artificial seaweed beds for the reconstitution of marine environments. To grow seaweed well, the formation of biofilms is an essential process in biofouling. This study focused on the formation of initial biofilms on steelmaking slag samples and analyzed the resulting bacterial communities using the next-generation sequencing technique. Three types of steelmaking slag were submerged in an area of Ise Bay in Mie Prefecture, Japan, for 3 and 7 days in the summer and winter seasons to allow the formation of biofilms. The bacterial communities of these biofilms were richer in sulfur-oxidizing bacteria compared to the biofilms formed on polyurethane sponges. It was found that Helicobacteraceae dominantly grew on the biofilms formed on the slag samples. This shows that steelmaking slags have potential to be used as artificial seaweed beds and marine water purifiers.

scholarly journals Analysis of targeted somatic mutations in pleomorphic carcinoma of the lung using next‐generation sequencing technique

2020 ◽  
Vol 11 (8) ◽  
pp. 2262-2269
Author(s):  
Saki Manabe ◽  
Rika Kasajima ◽  
Shuji Murakami ◽  
Yohei Miyagi ◽  
Tomoyuki Yokose ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Somatic Mutations ◽  
Pleomorphic Carcinoma ◽  
Next Generation ◽  
Sequencing Technique ◽  
Generation Sequencing

Metagenomic Next-Generation Sequencing Technique Helps Identify Cryptococcal Infection in the Rib

2019 ◽  
Vol 9 (4) ◽  
pp. e0367-e0367 ◽  
Author(s):  
Chaofan Zhang ◽  
Chaoxin Wang ◽  
Fei Chen ◽  
Zida Huang ◽  
Xinyu Fang ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Next Generation ◽  
Cryptococcal Infection ◽  
Sequencing Technique ◽  
Generation Sequencing

HLA-A, -B, -C, -DRB1 and -DQB1 allele and haplotype frequencies in Saudis using next generation sequencing technique

2013 ◽  
Vol 82 (4) ◽  
pp. 252-258 ◽  
Author(s):  
A. H. Hajeer ◽  
M. A. Al Balwi ◽  
F. Aytül Uyar ◽  
Y. AlHaidan ◽  
A. AlAbdulrahman ◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Next Generation ◽  
Haplotype Frequencies ◽  
Sequencing Technique ◽  
Dqb1 Allele ◽  
Generation Sequencing

scholarly journals Characterization of Leishmania Ulcers Microbiota Using Next Generation Sequencing: Leishmania Ulcers Microbiota

2021 ◽  
Vol 01 (1) ◽  
pp. 68-78
Author(s):  
Suheir Ereqat
Keyword(s):  
Next Generation Sequencing ◽  
Bacterial Communities ◽  
Pathogenic Bacteria ◽  
Healing Process ◽  
Leishmania Infection ◽  
Skin Microbiome ◽  
Next Generation ◽  
Subsequent Formation ◽  
Acinetobacter Species ◽  
Generation Sequencing

The human skin microbiome is a major source of bacteria in cutaneous leishmaniasis (CL) ulcers following the fall of the crust and the subsequent formation of a shallow depression in the epidermis and dermis of the skin. As a result, secondary bacterial infections are frequently observed which impair the healing process. Our study aimed at studying the bacterial communities in CL lesions using next-generation sequencing. A total of 298 patients (178 males and 120 females; median age of 17) presenting ulcerated skin lesions suspected with CL were included in this study. CL was confirmed in 153 (51%) cases by ITS1-PCR and/ or microscopy. Based on bacterial 16S rRNA-PCR, 92 samples were positive for the presence of bacteria, while 206 samples were negative and excluded from the microbiome study. A total of 925 Operational Taxonomic Units (OTUs) were identified and assigned to 215 genera. Despite insignificant difference in the microbiome composition between CL and non-CL lesions, the phylum level analysis revealed that Actinobacteria was significantly higher in CL ulcers while Protoeobacteria was significantly higher in non-CL ulcers (X2, P=0.039). The relative abundance of the most commonly encountered skin pathogens i. e E coli, Pseudomonas aeruginosa, Enterobacter, Enterococcus and Acinetobacter species were significantly higher in non-CL ulcers (X2, P<0.05) compared to Staphylococcus aureus and Proteus mirabilis which were higher in CL ulcers (P<0.05). Our data showed that bacterial communities did not cluster according to the Leishmania infection. Nonetheless, bacterial diversity was lower in CL compared to non-CL lesions. Presence of pathogenic bacteria in CL lesions such as S. aureus might exacerbate lesions, hinder diagnosis, and delay healing.

scholarly journals Next Generation Sequencing Technique and Its Application in Plant Virology

2019 ◽  
Vol 8 (2) ◽  
pp. 77-85
Author(s):  
zohreh Davoodi ◽  
jahangir heydarnejad ◽  
Hossein Masoomi ◽  
◽  
◽  
...  
Keyword(s):  
Next Generation Sequencing ◽  
Next Generation ◽  
Plant Virology ◽  
Sequencing Technique ◽  
Generation Sequencing
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